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Overview of Plant Stem Cell Products in Cosmetics Assoc. Prof. Dr. Ubonthip Nimmannit “Plant Stem Cell in Cosmetics” 25 August 2010 What are stem cells? • Unprogrammed cell • Differentiate into cells with specific function • Advantage – Regenerative medicine • Stimulate healing • Extended healthy life span Arabidopsis stem cells Reddy lab, UC Riverside Stem cells • Self-renewal • Generate differentiated cells General features of a stem-cell niche. Sablowski,TRENDS in Cell Biology, Vol.14,2004 Animal and Human Stem Cells • Embryonic stem cells – Pluripotent differentiation potential • Adult stem cells – To maintain and repair tissue • The same DNA and the same gene – Signal inside the cell – epigenetic signals tag DNA – Surrounding histone protein: regulate gene Plant stem cells • Shoot apical meristem • Root apical meristem – Epigenetic – Totipotent http://www.microscopyu.com/staticgallery/smz1500/generalizedplantcellstem.html Meristems • specialized proliferation tissue – the growing tip of roots and shoots – generate new cells Dinneny and Benfey, Cell 132, 2008 Root meristem • Quiescent center cells (4 cells) • Single layer of stem cells • Daughter cell (2 cells) – Cell renewal – Formation of mature tissue Dinneny and Benfey, Cell 132, 2008 Positioning the Root Stem Cell Niche Dinneny and Benfey, Cell 132, 2008 Shoot meristem • Organizing center – Several layers of cells – Plant growth hormone • Promote cell cycle activity • Promote growth • Shoot identity Dinneny and Benfey, Cell 132, 2008 Shoot apical meristem Branch Leaves Flower Plant shoot apical meristem (SAM) and root apical meristem (RAM) SC niches. The organizing-centre cells provide signals for maintenance of the overlying SCs, which are surrounded by a TA population of cells in the meristem peripheral zone (PZ). In the RAM, the quiescent centre (QC) is the source of SC maintenance signals. Singh and Bhalla, TRENDS in Plant Science, Vol.11, 2006 The role of stem cells in the skin • Epidermis – stratified epithelium – interfollicular epidermis • Hair follicles • Sebaceous glands The hair bulge contains multipotent stem cells that contribute to the lineages of the epidermis, the sebaceous gland and the hair follicle Schmid et al., SÖFW-Journal | 134 | 5-2008 Epidermal stem cell generates transit amplifying cells that differentiate to form the stratified layers Schmid et al., SÖFW-Journal | 134 | 5-2008 Explant Cell Colorless mass callus Individual/ small cell clusters in liquid culture Plant stem cell and their propagation in culture • Sterile condition • Standardized condition Plant stem cell products • • • • • Centella asiatica cell culture 20% Echinacea angustifolia cell culture 20% Buddleja davidii cell culture 20% Leontopodium alpinum cell culture 20% Malus domestica fruit cell culture extract Claims • • • • • • • • Stimulate human stem cell growth Maintains the self renewal capacity of stem cells Increases longevity of human skin stem cells Delays senescence of essential cells Combat chronological aging Preserve the youthful look and the vitality of skin neutralize, often reversing, the effects of aging on the skin protect stem cells from death due to UV overexposure • protects human skin stem cells from stress, thus decreasing wrinkles and producing younger, fresherlooking skin. Plant stem cell products Plant stem cell products STEM CELL ACTIVATOR with 20% Plant Stem Cells It’s all about PERCENTAGE It’s all about SYNERGY PhytoCellTec Malus Domestica (Swiss Apple) 20% Plant Stem Cells +Phytosteron - Wild Yam and Soy Bean Sterols Plant stem cell cream Lancome Paris Absolue Precious Cells Advanced Regenerating and Reconstructing Cream SPF15 Amore Pacific IOPE Plant Stem Cell Skin Renewal Eye Cream Price: $150 Price: $60 Plant stem cell cream emerginC protocell bio-active stem cell face cream. Price: $90 Serious Skin Care Replicate Renew Plant Stem Cell Double Power Concentrate Price: $46.50 Plant stem cell serum La Mer The Regenerating Serum Helena Rubinstein Prodigy Powercell Price: $250 Price: $120 Elions stem cell serum Price: $25 Plant stem cell : Anticellulite Adonia LegTone Serum Price: $120 Collistar Intensive Anticellulite Serum Price: $60 Thank you for your attention