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Cells: Prokaryotes v. Eukaryotes (extracts of 7-1 & ch19) Slide 1 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular The Discovery of the Cell Early Microscopes In 1665, Robert Hooke used an early compound light microscope to look at a thin slice of cork, a plant material. Slide 2 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular The Discovery of the Cell Early Microscopes At the same time, Anton van Leeuwenhoek used a microscope to observe pond water and other things. Daphnia – water insect Slide 3 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular The Discovery of the Cell What is the cell theory? In 1838, Matthias Schleiden – all plants were made of cells. In 1839, Theodor Schwann – all animals were made of cells. In 1855, Rudolph Virchow – new cells were created only from division of existing cells. These discoveries led to the cell theory. Copyright Pearson Prentice Hall Slide 4 of 31 End Show 7-1 Life Is Cellular The Discovery of the Cell Cell Theory is a SCIENTIFIC THEORY! Scientific Theory: A well tested explanation that unifies a broad range of observations. •Scientific Theory used to make accurate predictions about new observations and situations. •Scientific Theory can be revised according to new evidence Slide 5 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular The Discovery of the Cell cell theory • All living things are composed of one or more cells. • Cells are the basic units of structure and function in living things. » Use genetic code (DNA) » Over generations mutations accumulate and lead to changes over time » Get energy and materials from environment to run cellular metabolism » Respond to the external environment » Maintain homeostasis (stable internal environment) • New cells are produced from existing cells. » Grow and develop Copyright Pearson Prentice Hall Slide 6 of 31 End Show 7-1 Life Is Cellular Exploring the Cell Light microscope V. Electron microscope Electron Microscope Up to 100,000X Preserved cells only Scanning EM (3D image) Transmission EM (2D cross section) TEM Light micrograph (LM) Up to 1000X SEM Live or preserved cells Slide 7 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular DNA kept separate from the cell’s cytoplasm by the nuclear membrane Slide 8 of 31 Copyright Pearson Prentice Hall Figure End4.4 Show Scientist’s study of different organisms’ cell structure and DNA lead them to group organisms into 3 different domains ArchaeEubacteria: bacteria: Protists Plants Fungi Animals common bacteria UC Oldest bacteria type UC UC&MC MC UC&MC MC First Eukaryotes (Has nucleus) UC = unicellular MC= multicellular First cells (prokaryotic) 3.5 billion years ago 7-1 Life Is Cellular Prokaryotes and Eukaryotes Prokaryotes and Eukaryotes Prokaryote focus: The smallest, simplest life forms are prokaryotes—unicellular organisms that lack a nucleus. Biologists divided them into two different domains: the Eubacteria and the Archaebacteria. Slide 10 of 31 Copyright Pearson Prentice Hall End Show 19–1 Bacteria Classifying Prokaryotes Archaebacteria- Ancient bacteria Achaebacteria’s DNA is more similar to eukaryotes than eubacteria!. They live in extreme environments (why also known as extremeophiles) ●Methanogens live in oxygenfree environments, such as thick mud and animal digestive tracts. ●Other archaebacteria live in salty environments ● or in hot springs where water temperatures approach the boiling point. Slide 11 of 40 Copyright Pearson Prentice Hall End Show 19–1 Bacteria Classifying Prokaryotes Thermophiles of Yellowstone Produce Beautiful colors Grand Prismatic Spring Yellowstone. •This type of Extremophile requires temperatures above 45oC (113oF) to survive. •Wow: for most organisms, proteins and nucleic acids would denature at these hot temperatures. •Thermophiles source of enzymes used in DNA biotechnology Slide 12 of 40 Copyright Pearson Prentice Hall End Show 19–1 Bacteria Classifying Prokaryotes Eubacteria include organisms that live in a variety of environments, including: ●in fresh and salt water ● on land ●in the human body Slide 13 of 40 Copyright Pearson Prentice Hall End Show Structures of Prokaryotic Cells Nucleoid region – area where DNA is coiled in the cytoplasm. DNA is in direct contact with the rest of the cell Ribosome Plasmid – smaller circular DNA molecules – stores genes easily shared with other bacteria Ribosomes – where proteins are made cell membrane – encloses the cytoplasm of the prokaryotic cell, regulates what chemicals enter and leave the cell. Flagella Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings DNA Plasmid Pili Structures of the Prokaryotic Cell Cell wall – rigid, composed of lipids, carbohydrates and protein. Protects the cell and maintains its shape Pili – short surface projections found in some prokaryotes. Helps attach bacteria to surfaces Flagella – long whiplike extensions found in some prokaryotes. Propel cell through liquid environments Not all bacteria the a same shape! •Bacilli (rod) •Cocci (round) •Spirilla (spiral) Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings 19–1 Bacteria Importance of Bacteria Bacteria are vital to the living world. Autotrophs: Some are producers that capture energy by photosynthesis. Heterotrophs: using living organisms as food source. ●decomposers ●Nitrogen fixing bacteria ●Pathogens: cause disease • Some bacteria damage the cells and tissues directly by breaking down the cells for food. • Others By produce toxins that makes you sick Slide 16 of 40 Copyright Pearson Prentice Hall End Show 19–3 Diseases Caused by Bacteria and Viruses Bacterial Disease Bacterial Disease in Humans Bacterial Diseases Growth of pathogenic bacteria disrupts the body’s equilibrium by interfering with its normal activities and producing disease. Antibiotics Chemicals that kill bacteria w/o harming your cells –Ex: Penicillin disrupts bacterial cell wall production –Does not work on Viral infections Slide 17 of 29 Copyright Pearson Prentice Hall End Show Importance of Bacteria 19–1 Bacteria Still other bacteria have human uses. ●foods ●Genetically engineered bacteria used to produce medicine ●production of some vitamins especially vitamins K in human intestines Slide 18 of 40 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Compare & Contrast: What are the characteristics of prokaryotes and eukaryotes? Slide 19 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Prokaryotes Prokaryotic cells have genetic material that is not contained in a nucleus. –do not have membrane-bound organelles. –cells are generally smaller and simpler than eukaryotic cells. –Bacteria (both archaebacteria and eubacteria) are prokaryotes. Slide 20 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Prokaryotes and Eukaryotes All cells have DNA to store hereditary information but where is it in the cells? Eukaryotes have a nucleus – DNA surrounded by a a nuclear envelope membrane separating DNA from the rest of the cell Eukaryotes have membrane bound organelles – like mitochondria and Endoplasmic Reticulum Slide 21 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Eukaryotes ●Cells highly specialized ●In multicellular species groups of cells can form tissues Slide 22 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Eukaryotic cells: Build the structures of Plants, animals, fungi, and protists. Slide 23 of 31 Copyright Pearson Prentice Hall End Show 7-1 Life Is Cellular Prokaryotes and Eukaryotes Compare and contrast Pro Both Eu Slide 24 of 31 Copyright Pearson Prentice Hall End Show