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The Five-Kingdom System Note bias towards large things Kingdom Protista Kingdom Fungi Kingdom Animalia Kingdom Animalia Arthropod Vectors Aedes Aegyti Phlebotmine Sandfly Assorted Ticks DOMAIN ARCHAEA Giardia Trypanosoma Euglena Entamoebae Slime molds Dinoflagellates Ciliates Diatoms Brown algae Red algae Green algae Plants Fungi Animals pJP78 pJP27 pSL17 pSL12 Pyrodictium Sulfolobus Thermoproteus Methanopyrus Methanobacterium Thermococcus Methanococcus Archaeoglobus Halococcus Aquifex Thermus Thermotoga Gram-positive bacteria Green sulfur bacteria Purple bacteria Cyanobacteria Flavobacteria Kingdom Monera DOMAIN BACTERIA DOMAIN EUKARYA Stromatolites (mounds of photosynthetic bacteria) Three Cellular Domains 16S rRNA Three Cellular Domains Hierarchical Classification Kingdom Animals Phylum Chordates Class Mammals Order Primates Family Hominids Here we will be most concerned with Genus species, and strains Homo genus, e.g., Species sapiens (Humans) Escherichia coli O157:H7 Dichotimous Key 1a. Money is made of paperBills, Go to 2 1b. Money is not made of paperCoins, Go to 3 2a. Bill has Washington on frontOne Dollar Bill 2b. Bill does not have Washington on frontTen Dollar Bill 3a. Coin is made of copperPenny 3b. Coin is not made of copperGo to 4 4a. Coin has FDR on frontDime 4b. Coin does not have FDR on frontQuarter Numerical Taxonomy Genetic Homology Genetic homologies (similarities) can be determined by looking at: • Base compositions • DNA-DNA or DNA-RNA Hybridization • DNA or RNA sequencing Base Composition DNA Sequencing RNA Sequencing DNA Hybridization Southern Blotting Visualized DNA