* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Document
Deoxyribozyme wikipedia , lookup
Non-coding DNA wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Point mutation wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Epigenetics of human development wikipedia , lookup
Genomic imprinting wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Hardy–Weinberg principle wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Genome (book) wikipedia , lookup
X-inactivation wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Helitron (biology) wikipedia , lookup
Quantitative trait locus wikipedia , lookup
Designer baby wikipedia , lookup
History of genetic engineering wikipedia , lookup
Dominance (genetics) wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Fig. 10-3a Leaf cross section Vein Mesophyll Stomata Chloroplast CO2 O2 Mesophyll cell 5 µm Fig. 10-3b Chloroplast Outer membrane Thylakoid Stroma Granum Thylakoid space Intermembrane space Inner membrane 1 µm Concept Map - Photosynthesis Fig. 10-4 Reactants: Products: 12 H2O 6 CO2 C6H12O6 6 H2O 6 O2 Oxidation & Reduction The Splitting of Water • Chloroplasts split H2O into hydrogen and oxygen, incorporating the electrons of hydrogen into sugar molecules Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings When light strikes an object: • Transmitted • Absorbed (primarily red & blue light) • Reflected (green light) 3 major events occur during photosynthesis 1. Absorption of light energy 2. Conversion of light energy to chemical (ATP & NADPH) 3. Storage of chemical energy in sugars These events occur during: -light rxn. (events 1,2) -dark rxn. (event 3) Photosynthetic Pigments: The Light Receptors • Pigments are substances that absorb visible light • Different pigments absorb different wavelengths • Wavelengths that are not absorbed are reflected or transmitted • Leaves appear green because chlorophyll reflects and transmits green light Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 10-7 Light Reflected light Chloroplast Absorbed light Granum Transmitted light Fig. 10-6 10–5 nm 10–3 nm 103 nm 1 nm Gamma X-rays rays UV 106 nm Infrared 1m (109 nm) Microwaves 103 m Radio waves Visible light 380 450 500 Shorter wavelength Higher energy 550 600 650 700 750 nm Longer wavelength Lower energy Fig. 10-5-4 CO2 H2O Light NADP+ ADP + P i Light Reactions Calvin Cycle ATP NADPH Chloroplast O2 [CH2O] (sugar) • There are two types of photosystems in the thylakoid membrane • Photosystem II (PS II) functions first (the numbers reflect order of discovery) and is best at absorbing a wavelength of 680 nm • The reaction-center chlorophyll a of PS II is called P680 Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings The Nature of Sunlight • Light is a form of electromagnetic energy, also called electromagnetic radiation • Like other electromagnetic energy, light travels in rhythmic waves • Wavelength is the distance between crests of waves • Wavelength determines the type of electromagnetic energy Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings • The electromagnetic spectrum is the entire range of electromagnetic energy, or radiation • Visible light consists of wavelengths (including those that drive photosynthesis) that produce colors we can see • Light also behaves as though it consists of discrete particles, called photons Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 9-2 Light energy ECOSYSTEM Photosynthesis in chloroplasts CO2 + H2O Organic +O molecules 2 Cellular respiration in mitochondria ATP ATP powers most cellular work Heat energy • C6H12O6 + 6 O2 6 CO2 + 6 H2O + Energy • Energy = (ATP + heat) Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings The Stages of Cellular Respiration: A Preview • Cellular respiration has three stages: – Glycolysis (breaks down glucose into two molecules of pyruvate) – The citric acid cycle (completes the breakdown of glucose) or Krebs Cycle – Oxidative phosphorylation (accounts for most of the ATP synthesis) or ETC Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 9-6-3 Electrons carried via NADH and FADH2 Electrons carried via NADH Citric acid cycle Glycolysis Pyruvate Glucose Oxidative phosphorylation: electron transport and chemiosmosis Mitochondrion Cytosol ATP ATP ATP Substrate-level phosphorylation Substrate-level phosphorylation Oxidative phosphorylation Concept 9.2: Glycolysis harvests chemical energy by oxidizing glucose to pyruvate • Glycolysis (“splitting of sugar”) breaks down glucose into two molecules of pyruvate • Glycolysis occurs in the cytoplasm and has two major phases: – Energy investment phase – Energy payoff phase Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings • In alcohol fermentation, pyruvate is converted to ethanol in two steps, with the first releasing CO2 • Alcohol fermentation by yeast is used in brewing, winemaking, and baking Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings Fig. 14-15b 1st generation (grandparents) 2nd generation (parents, aunts, and uncles) Ww ww ww Ww ww ww Ww Ww Ww ww 3rd generation (two sisters) WW or Ww Widow’s peak ww No widow’s peak (a) Is a widow’s peak a dominant or recessive trait? Fig. 14-15c 1st generation (grandparents) Ff 2nd generation (parents, aunts, and uncles) FF or Ff ff Ff ff ff Ff Ff Ff ff ff FF or Ff 3rd generation (two sisters) Attached earlobe Free earlobe (b) Is an attached earlobe a dominant or recessive trait? Fig. 14-3-3 EXPERIMENT P Generation (true-breeding parents) F1 Generation (hybrids) Purple flowers White flowers All plants had purple flowers F2 Generation 705 purple-flowered plants 224 white-flowered plants Table 14-1 Fig. 14-4 Allele for purple flowers Locus for flower-color gene Allele for white flowers Homologous pair of chromosomes Fig. 14-5-3 P Generation Purple flowers Appearance: Genetic makeup: PP Gametes: White flowers pp p P F1 Generation Appearance: Genetic makeup: Purple flowers Pp 1/ 2 Gametes: 1/ 2 P Sperm F2 Generation P p PP Pp Pp pp P Eggs p 3 1 p Fig. 14-6 3 Phenotype Genotype Purple PP (homozygous) Purple Pp (heterozygous) 1 2 1 Purple Pp (heterozygous) White pp (homozygous) Ratio 3:1 Ratio 1:2:1 1 Fig. 14-7 TECHNIQUE Dominant phenotype, Recessive phenotype, unknown genotype: known genotype: PP or Pp? pp Predictions If PP Sperm p p P Pp Eggs If Pp Sperm p p or P Pp Eggs P Pp Pp pp pp p Pp Pp RESULTS or All offspring purple 1/2 offspring purple and 1/2 offspring white The Law of Independent Assortment • Mendel derived the law of segregation by following a single character • The F1 offspring produced in this cross were monohybrids, individuals that are heterozygous for one character • A cross between such heterozygotes is called a monohybrid cross Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-8 EXPERIMENT YYRR P Generation yyrr Gametes YR F1 Generation yr YyRr Hypothesis of dependent assortment Predictions Hypothesis of independent assortment Sperm or Predicted offspring of F2 generation 1/ 4 Sperm 1/ YR 1/ 2 2 yr 1/ 4 1/ 2 YR 1/ 4 Yr 1/ 4 yR 1/ 4 yr YR YYRR YYRr YyRR YyRr YYRr YYrr YyRr Yyrr YyRR YyRr yyRR yyRr YyRr Yyrr yyRr yyrr YR YYRR Eggs 1/ 2 YyRr 1/ 4 Yr Eggs yr YyRr 3/ 4 yyrr 1/ 4 yR 1/ 4 Phenotypic ratio 3:1 1/ 4 yr 9/ 16 3/ 16 3/ 16 1/ 16 Phenotypic ratio 9:3:3:1 RESULTS 315 108 101 32 Phenotypic ratio approximately 9:3:3:1 Fig. 14-8a EXPERIMENT YYRR P Generation yyrr Gametes YR F1 Generation yr YyRr Hypothesis of independent assortment Hypothesis of dependent assortment Predictions Sperm or Predicted offspring of F2 generation 1/ 4 Sperm 1/ 2 YR 1/ 2 1/ 4 Yr yR 1/ 4 yr YR YYRR YYRr YyRR YyRr YYRr YYrr YyRr Yyrr YyRR YyRr yyRR yyRr YyRr Yyrr yyRr yyrr YR YYRR Eggs 1/ 2 1/ 4 yr 1/ 4 1/ 2 YR YyRr 1/ 4 Yr Eggs yr yyrr YyRr 3/ 4 1/ 4 yR 1/ 4 Phenotypic ratio 3:1 1/ 4 yr 9/ 16 3/ 16 3/ 16 1/ 16 Phenotypic ratio 9:3:3:1 Fig. 14-8b RESULTS 315 108 101 32 Phenotypic ratio approximately 9:3:3:1 • Using a dihybrid cross, Mendel developed the law of independent assortment • The law of independent assortment states that each pair of alleles segregates independently of each other pair of alleles during gamete formation • Strictly speaking, this law applies only to genes on different, nonhomologous chromosomes – not to linked genes – gene on same chromosome • Genes located near each other on the same chromosome tend to be inherited together Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Extending Mendelian Genetics for a Single Gene • Inheritance of characters by a single gene may deviate from simple Mendelian patterns in the following situations: – When alleles are not completely dominant or recessive – When a gene has more than two alleles – When a gene produces multiple phenotypes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Degrees of Dominance • Complete dominance occurs when phenotypes of the heterozygote and dominant homozygote are identical • In incomplete dominance, the phenotype of F1 hybrids is somewhere between the phenotypes of the two parental varieties • In codominance, two dominant alleles affect the phenotype in separate, distinguishable ways Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-10-3 P Generation Red CRCR White CWCW CR Gametes CW Pink CRCW F1 Generation Gametes 1/2 CR 1/ CW 2 Sperm 1/ 2 CR 1/ 2 CW F2 Generation 1/ 2 CR Eggs 1/ 2 CRCR CRCW CRCW CWCW CW Inheritance of Sex-Linked Genes • The sex chromosomes have genes for many characters unrelated to sex • A gene located on either sex chromosome is called a sex-linked gene • In humans, sex-linked usually refers to a gene on the larger X chromosome Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings • Sex-linked genes follow specific patterns of inheritance • For a recessive sex-linked trait to be expressed – A female needs two copies of the allele – A male needs only one copy of the allele • Sex-linked recessive disorders are much more common in males than in females Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 15-7 XNXN Sperm Xn XnY Sperm XN Y Eggs XN X N X n X NY XN X NXn XNY (a) XNXn XNY XNXn Sperm Xn Y XnY Y Eggs XN XNXN XNY Eggs XN X NX n X NY XnXN XnY Xn XnXn XnY Xn (b) (c) Fig. 14-11 Allele IA IB Carbohydrate A B i none (a) The three alleles for the ABO blood groups and their associated carbohydrates Genotype Red blood cell appearance Phenotype (blood group) IAIA or IA i A IBIB or IB i B IAIB AB ii O (b) Blood group genotypes and phenotypes •Rh factor is a protein called an antigen on RBC •An antigen is any substance that the body considers "foreign" and thus stimulates the body to produce antibodies against it. •Rh factor, like the blood types A, B, and O, is inherited from one's parents. •A simple blood test can determine blood type, including the presence of the Rh factor. •About 85 percent of white Americans and 95 percent of African Americans have the Rh factor and are known as Rh-positive. •Those without the Rh factor are Rh-negative. Extending Mendelian Genetics for Two or More Genes • Some traits may be determined by two or more genes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Polygenic Inheritance • Quantitative characters are those that vary in the population along a continuum • Quantitative variation usually indicates polygenic inheritance, an additive effect of two or more genes on a single phenotype • Skin color in humans is an example of polygenic inheritance Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Fig. 14-13 AaBbCc AaBbCc Sperm 1/ Eggs 1/ 8 1/ 8 1/ 8 1/ 8 1/ 1/ 8 1/ 1/ 8 8 1/ 8 1/ 64 15/ 8 1/ 1/ 8 8 8 1/ 8 1/ 8 1/ 8 8 1/ Phenotypes: 64 Number of dark-skin alleles: 0 6/ 64 1 15/ 64 2 20/ 3 64 4 6/ 64 5 1/ 64 6 DNA – A Historical Perspective • 1865 – Gregor Mendel – “Father of Heredity” • 1869 – Johann Miescher (Swiss biochemist) – isolates DNA from WBC • 1902 – Walter Sutton – American Geneticist – Columbia U. Theory of the Chromosome • 1928 – Frederick Griffith – British Bacteriologist – discovers transformational factor • 1944 – Oswald Avery et al. - Canadian-born American physician – shows that the transformational factor was not a protein but DNA • 1952 – Alfred Hershey & Martha Chase – provide conclusive evidence that DNA is the transformational factor • 1952 – Rosalind Franklin & Maurice Wilkins – use x-ray diffraction to analyze DNA • 1953 – James Watson & Francis Crick construct double helix model of DNA Fig. 16-6 (a) (b) Fig. 16-8 Fig. 16-5 Sugar–phosphate backbone 5 end Carbon 1 – bonds to nitrogen base Nitrogenous bases Purines Thymine (T) Pyrimidines Carbon 3 – bonds to next nucleotide Adenine (A) Carbon 5 – bonds to phosphate group Cytosine (C) DNA nucleotide Phosphate Sugar (deoxyribose) 3 end Guanine (G) #1. TECHNIQUE 5 µm #2 Key Maternal set of chromosomes Paternal set of chromosomes Two sister chromatids of one replicated chromosome Two nonsister chromatids in a homologous pair Centromere Pair of homologous chromosomes (one from each set) #3 Key Haploid gametes (n = 23) Haploid (n) Egg (n) Diploid (2n) Sperm (n) C. A. D. B. E. Mitosis and development Multicellular diploid adults (2n = 46) Prophase I Metaphase I C. Anaphase I Sister chromatids remain attached B Homologous chromosomes separate Homologous chromosomes Fragments of nuclear envelope Microtubule attached to kinetochore D #5 A. B. #6 SUMMARY Property Mitosis Meiosis DNA replication A. When does this happen??? Occurs during interphase before meiosis I begins Number of divisions One, including prophase, metaphase, anaphase, and telophase B. How many divisions?? Pairing of homologous chromosomes Does not occur C. Exactly when does this occur? Number of daughter cells and genetic composition Two, each diploid (2n) and genetically identical to the parent cell D. # of daughter cells?? Role in the animal body Enables multicellular adult to arise from zygote; produces cells for growth, repair, and, in some species, asexual reproduction Produces gametes; reduces number of chromosomes by half and introduces genetic variability among the gametes 7. Possibility 2 Possibility 1 Two equally probable arrangements of chromosomes at metaphase I Metaphase II Daughter cells Combination 1 Combination 2 Combination 3 Combination 4 #8. Enzymes involved in DNA Replication & Transcription Enzyme Function A. “molecular zipper” – unwinds double helix; breaks hydrogen bonds that holds base pairs together B. “molecular swivel”- relieves overwinding stress on DNA strands by working ahead of helicase and breaking, swiveling and rejoining small sections of the DNA molecule C. Using a parent DNA strand, adds free-floating nucleotides (A, T, G, & C’s) covalently to the new strand being constructed. D. “molecular glue” – joins fragments of the New DNA strand together E. Uses one strand of DNA as a template to construct mRNA – adds free-floating nucleotide F. Fixes mistakes on DNA molecule There are several different mechanisms (or modes) of natural selection that involve changes in niches. These mechanisms include directional selection, disruptive selection, and stabilizing selection. State which of these is involved in each of the following examples and predict the change (if any) produced by each. a.Most giraffes in an area are the same height. Adult giraffes much taller or shorter than this height are unusual. The type and typical height of the vegetation does not change. In an area, some chipmunks have lightcolored fur and some have dark fur, but the largest number have medium-colored fur. The ground is usually a mediumbrown color in winter, however, the climate drastically changes, and snow becomes unusually frequent and heavy. Most grasshoppers in an area are medium-green in color. There are a smaller number of them that are light green and some are brownish. A predator is introduced into the area and it has a preference for medium-green grasshoppers.