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Bottom-up NanoBiotechnology: from Membrane proteins to BioSolar Power October 12th 2006 Andreas Mershin, PhD & Brian Cook P.I. Shuguang Zhang, PhD Lab for Molecular Self Assembly [email protected], [email protected] http://web.mit.edu/lms/www/ Self-Assembling Peptides stabilize membrane proteins Nano-Bio-PhotoVoltaic Dog on a chip Solid State Devices Andreas Mershin Center For Biomedical Engineering Surfactant/detergent peptides can be designed to stabilize membrane proteins in unnatural environments such as bioelectronic circuits or sensors. Peptide stabilization can help in crystallization studies Andreas Mershin Center For Biomedical Engineering 1st Generation (flat monolayer) devices now in the Boston Museum of Science. Good concept but very low power and efficiency <1µm Nature, Boston Globe AP,Reuters, CNN, NYT, FT, BBC, etc. Biomimicry resonates with people Andreas Mershin Center For Biomedical Engineering Electric Nanoforest: ZnO nanowires are transparent, conducting tree-trunks ZnO transparent and conducting nanowires grown using CVD (high T) or wet deposition (low temp) High surface area, transparent, conducting, biofriendly, enhancement factor ~200-2000 1 Aold 1 2Rh 200 2 Anew (R g ) Andreas Mershin Center For Biomedical Engineering Self-assembled “velcro” + PS1 natural PsaD + ZnO-PS1 ZnO-PsaD IV ITO ZnO PS-I ZnO-binding peptide sequence (GLHIPTGSSYSHR) • Direct attachment (less R, more I) ZnO NW ITO GLASS Device architecture Andreas Mershin Center For Biomedical Engineering Olfactory receptors: smell background The mammalian nose has the ability to rapidly distinguish between an enormous range of small molecules at low concentrations The proteins responsible are olfactory receptors (ORs), a large class of sensory proteins which function combinatorially to allow the brain to discriminate odors Currently, the molecular mechanism by which this receptor set functions is unknown Andreas Mershin Center For Biomedical Engineering Olfactory receptors: purification Tagged and optimized OR genes are synthesized (PCR-based gene assembly) then inserted into “producer” cell lines OR production is then “induced” in cell culture and the proteins harvested out and purified via the affinity tags (using HPLC) Olfactory receptors: structural & functional measurement Structure: 2º confirmed via CD spectroscopy, 3º to be done with X-ray crystallography Function: odorant binding via SPR (Biacore), plus other methods Beyond: construction of prototype smell “biosensors” utilizing OR proteins Andreas Mershin Center For Biomedical Engineering