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Quiz#3
LC710
9/29/10
name____________
Q1(1pt) Given the following protein:
Nt- MNTSFSMLAAYMFLLIMLGFAINEAAFLTLYVTVLNYILLNLAVADEFRVFGGFTAT
LYTSLGFFATLGGEIEALHWSLDVLAIERYVVVAIMGVAFTWVMALACAAVSLSFV
IYMFVVHFIDIALRIVEIFFCYGMVIIMVIADFLICWELPYAGVAFYIFTHAFFAKKR
TSAVYNPVIYIMMN-Ct
Circle the potential transmembrane domains. There are more than 1.
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Q2(1pt) Given the following Kyte-Doolittle hydropathy plot:
Circle on the number line where transmembrane regions reside.
Q3(3pt) Given the following protein:
MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML *
* = STOP
You want to PCR up this conserved protein from an unknown species.
-Design a fully degenerate oligo to the underlined Nt amino acids and include an
extra EcoR1 site (GAATTC) at the 5’ end. This is a top strand oligo.
-Design a fully degenerate oligo to the underlined Ct amino acids and include an
extra BamHI site (GGATCC) at the 5’ end. This is a bottom strand oligo.
Genetic Code:
Write both oligos 5’ to 3’.
Make sure to annotate 5’ and 3’ ends of your two oligos.
(1pt)
(2pt)
Oligo 1:_______________________________
Oligo 2:_______________________________
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Q4(1pt) Given the following Gene below:
Using the following list of symbols:
ATG, STOP, SD, SA, 5’UTR, 3’UTR
Completely label the gene below with these symbols
KEY: SD: splice donor
SA: splice acceptor
3’
UTR: untranslated
5’
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region
(1pt) Extra Credit: AATAAA is a polyA recognition site.
Using an arrow show approximate position in gene above.