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11-442-C025
Monoclonal Antibody to LAT
Purified Antibody (0.025 mg)
Clone:
LAT-1D1
Isotype:
Mouse IgG2a
Specificity:
The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells
(LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK
cells, mast cells and platelets.
Regulatory Status:
RUO
Immunogen:
Bacterially expressed full-length rat LAT
Species Reactivity:
Mouse, Rat
Negative Species:
Human
Application:
Western Blotting
Recommended dilution: 5-10 µg/ml
Positive control: mouse spleen
Immunoprecipitation
Immunocytochemistry
Purity:
> 95% (by SDS-PAGE)
Purification:
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Concentration:
1 mg/ml
Storage Buffer:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage / Stability:
Store at 2-8oC. Do not freeze. Do not use after expiration date stamped on vial
label.
Expiration:
See vial label
Lot Number:
See vial label
Background:
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated
transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast
cells and platelets. After immunoreceptor triggering, LAT becomes multiply
tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking
sites for downstream signaling molecules. LAT is essential for TCR-dependent T
cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of
early thymocytes. It is also involved in NK cell signaling and platelet activation.
For laboratory research only, not for drug, diagnostic or other use.
EXBIO Praha | Nad Safinou II 341 | 252 42 Vestec u Prahy | Czech Republic
Tel: +420 261 090 666 | Fax: +420 261 090 660 | [email protected] | www.exbio.cz
References:
Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito
T: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular
signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007
Jun;27(12):4406-15.
*Tolar P, Tumova M, Draber P: New monoclonal antibodies recognizing the
adaptor protein LAT. Folia Biol (Praha). 2001;47(6):215-7.
*Volná P, Lebduska P, Dráberová L, Símová S, Heneberg P, Boubelík M, Bugajev
V, Malissen B, Wilson BS, Horejsí V, Malissen M, Dráber P: Negative regulation of
mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct
18;200(8):1001-13.
*Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma
membrane microdomains and its consequences for mast cell signaling. Eur J
Immunol. 2006 Oct;36(10):2795-806.
*Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling
molecules as detected by electron microscopy on plasma membrane sheets
isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec
1;328(1-2):139-51.
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer
either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held
responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to
EXBIO´s term and conditions which are available at www.exbio.cz.
For laboratory research only, not for drug, diagnostic or other use.
EXBIO Praha | Nad Safinou II 341 | 252 42 Vestec u Prahy | Czech Republic
Tel: +420 261 090 666 | Fax: +420 261 090 660 | [email protected] | www.exbio.cz