Download Effects of electric field on mast cells Dan Zhu, Zu-Hui Wu, Ji

Effects of electric field on mast cells
Dan Zhu, Zu-Hui Wu, Ji-Yao Chen and Lu-Wei Zhou
Soft-matter Laboratory, State Key Laboratory of Surface Physics, and Department of Physics
Fudan Univiversity, Shanghai, China
Mast cell is a kind of immune cells which is sensitive to physical stimuli such as electric field, however the mechanism of mast
cells response to electric field is still not fully understood. This study aims to explore the mechanism of calcium release and
activation of TRPV channels stimulated by electric field. A stable AC electric field was applied to mast cells, the cytosol calcium
([Ca2+]c) was measured as well as the corresponding electric field. Calcium oscillation appeared in mast cell when stimulated by
AC electric field, the mechanism is to be studied.
II. METHODOLOGY
I. BACKGROUND
Measurements of [Ca2+]i
——confocal fluorescence
microscopy
What is the influence of
electric field mast cells ?
IV. Experiment results 2
mast cell
3
control
10V 200Hz (hepes)
III. Experiment results 1
10V 100Hz (saline)
fluorescence
2s
70 s
140s
fluorescence
10V 0.1hz (saline)
210 s
the peak
2
1.20
1.18
1.16
1.14
1.12
1.10
1.08
1.06
1.04
1.02
1.00
0.98
0.96
0.94
0.92
0.90
0
100
200
300
400
500
600
time/s
1
0
100
200
300
400
500
600
time/s
350 s
400s
450 s
500s
530s
560 s
600s
fluorecence
270 s
(concentration of calcium AC 20V 100Hz)
frequence
V. Conclusions
1.The concentration of calcium varies in mast cells when stimulated by AC electric field.
2.A model is to be built to simulated the phenomenon .
Reference
[1] ZuHui Wu , Dan Zhu , JiYao Chen, LuWei Zhou. The effects of thermal Stimulus on Intracellular Calcium Change and Histamine Releases in RBL-2H3 Mast Cells. Journal of Applied
Physics. 2012 .In press .
[2] Shaka S. Scarlett, Jody A.White. Regulation of intracellular calcium concentration by nanosecond pulsed electric field. BIOCHIMICA ET BOIPHYSICA ACTA, 1788(2009),1168-1175.
[3] Kyle E. Hammerick, Michael T. Longaker, In vitro effects of direct current eletric field on adipose-derived stromal cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH
COMMUNICATIONS, 379(2010), 12-17