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BIS101-003 Office hours/review sessions for Midterm I Midterm I - Tuesday, October 21 Office hours/review sessions: R Oct 16 1-2pm 145ABriggs Rima F Oct 17 12-1pm 3061SLB Engebrecht Su Oct 19 6-8pm 2205Haring Engebrecht M Oct 20 12-1pm 3061SLB Rebecca No calculators or cell phones during test, use pen if you want the possibility to request a regrade Review Quiz Scores: 291 Missing:2 High: 100.00% Low: 0.00% Median:95.00% Mean: 92.43% Standard Deviation:9.95 Critical Genetic Link Found Between Human Taste Differences And Nicotine Dependence http://www.sciencedaily.com/releases/2008/10/081014111301.htm Two interacting genes related to bitter taste sensitivity, TAS2R16 and TAS2R38 Tiny Worm Provides Model For The Genetics Of Nicotine Dependence http://www.sciencedaily.com/releases/2006/11/061103083610.htm CQ1 A cross is made in E. coli between an Hfr strain that is leu+ his+ gly+ Strs and an F- strain that is leu- his- gly- StrR. Interrupted mating studies show that his+ enters the recipient last. In an interrupted mating between the same two strains, what medium would you select the conjugates on before screening for recombinants? A. B. C. D. Medium containing his and lacking streptomycin Medium containing his and containing streptomycin Medium lacking his and containing streptomycin Medium lacking his and lacking streptomycin CQ2 You select on medium lacking his and containing streptomycin and then test by replica plating for the presence of the leu+ and gly+ alleles. The following numbers of individuals are found for each genotype: gly+ leu+ his+ = 259 gly- leu+ his+ = 1 gly+ leu- his+ = 11 gly- leu- his+ = 29 300 What is the gene order? A. gly leu his B. leu gly his C. gly his leu CQ3 You select on medium lacking his and containing streptomycin and then test by replica plating for the presence of the leu+ and gly+ alleles. The following numbers of individuals are found for each genotype: gly+ leu+ his+ = 259 gly- leu+ his+ = 1 leu gly his gly+ leu- his+ = 11 gly- leu- his+ = 29 300 What are the map distances between these three genes? A. leu 4mu gly 10mu his B. leu 10mu gly 4mu his C. leu 10mu gly 10mu his T4 bacteriophage CQ4 In a generalized transduction experiment, the T1 phage particles growing on E. coli with the genotype val+ ala+ trp+ are collected and allowed to infect E. coli cells with the genotype val- ala- trp-. The cotransduction frequencies are calculated for the different gene combinations: ala-trp = 0.2 ala-val = 0.8 val-trp = 0.6 The gene order is: A. ala - trp - val B. ala - val - trp C. val - ala - trp