Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download lecture 3
Document related concepts
Genome evolution wikipedia , lookup
Gene expression programming wikipedia , lookup
Genetic engineering wikipedia , lookup
Primary transcript wikipedia , lookup
Gene expression profiling wikipedia , lookup
Genome (book) wikipedia , lookup
Polycomb Group Proteins and Cancer wikipedia , lookup
X-inactivation wikipedia , lookup
Gene therapy of the human retina wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Epigenetics of human development wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
RNA silencing wikipedia , lookup
History of genetic engineering wikipedia , lookup
Koinophilia wikipedia , lookup
Genome editing wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Oncogenomics wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Designer baby wikipedia , lookup
Point mutation wikipedia , lookup
Mir-92 microRNA precursor family wikipedia , lookup
Microevolution wikipedia , lookup
Transcript
• What did you learn from surfing FlyBase? • Why do the inversions in Balancer chromosomes greatly reduce the frequency of crossing over in meiosis? • Autonomous vs non autonomous Nonautonomous • Domineering - mutant cells disrupt the development of neighboring wild type cells. • Submissive - wild type neighbors rescue mutant cells. • FLP/FRT can also be used to remodel chromosomes. RNAi in flies ds RNA in cultured cells. http://flyrnai.org/RNAi_goals.html RNAi in flies 1. Express a fold back RNA from a transgene. 2. This results in tissue/temporal specific gene knockdowns. 3. Collections of transgenic flies are available that contain transgenes to knockdown the expression of most fly genes. Gene targeting • Until recently not available in flies. • One difficulty is that one cannot select for a targeted cell. • A technique has now been developed that has been shown to work on a number of genes. • Technique requires first getting a germ line transformant for the knock out construct. • The DNA for targeting is excised by the activity of the FLP/FRT site specific recombination system. • The excised circle is then cut by the induced expression of a very rare cutting RE. • High rate of homologous targeting. In Vivo Imaging • A number of developmental stages/tissues provide opportunities for in vivo imaging. Genetic Screens • Recessive lethal mutations The isolation of recessive visible mutations mutagenize th st X fz in ri/TM3 screen for flies that are phenotypically fz or in. these flies would be fz*th st/fz in ri or th st in*/fz in ri The isolation of recessive lethal mutations cn bw * cn bw individual * cross siblings 2 treat with mutagen (e.g. ems) X Gla/CyO cn bw/CyO * cn bw/CyO X Gla/CyO X * cn bw/CyO X cn bw/ cn bw CyO/CyO cn bw/CyO Look for vials with no straight winged flies * * * Flp/FRT • High efficiency of FLP/FRT clone induction has allowed new types of mutant screens.
