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CHAPTER 17 HANDOUT Mapping Chromosomes BLM 17.2.3 Purpose: To construct a chromosome map. Steps to determine map distance Step Example Perform a cross between a fly that is known to be heterozygous for both traits and a fly that is homozygous recessive for both traits. Collect a large number of F1 flies from the cross. Determine the number of flies with each of the possible phenotypes. Calculate the total number of recombinant F1 phenotypes as a percentage of the total number of F1 flies. This gives you the recombination frequency and the number of map units separating the two genes. PpVv × ppvv Possible non-recombinant phenotypes: • purple eye, vestigial wing • normal eye, normal wing Possible recombinant phenotypes: • purple eye, normal wing • normal eye, vestigial wing number of recombinant types 100% total number of offspring Part A: Constructing a Chromosome Map Procedure 1. Using the table above as a guide, determine the map distance between the linked genes for eye colour and wing type in the following experiment. You perform the following cross: PpVv × ppvv. You count 1000 offspring in the F1 generation. You find that 406 of the flies have normal eyes and normal wings, 398 have purple eyes and vestigial wings, 96 have normal eyes and vestigial wings, and 100 have purple eyes and normal wings. 2. From previous research findings, you know that the distance between the gene for eye colour and the gene for body colour is 12.2 map units, and the gene for body colour is 7.4 map units away from the gene for wing type. All three genes are on the same chromosome. Draw a chromosome map showing the relative distances between the three linked genes. Analysis 1. You conduct the same cross again, but this time you get an almost exact 1:1 ratio of flies with normal eyes and normal wings to flies with purple eyes and vestigial wings. There are no recombinant types. Provide two explanations that might account for these results. 2. Linkage data has been used to map genes on the chromosomes of Drosophila melanogaster. Do you think such data could be used to map human chromosomes? Explain your reasoning. Copyright © 2007, McGraw-Hill Ryerson Limited, a Subsidiary of the McGraw-Hill Companies. All rights reserved. This page may be reproduced for classroom use by the purchaser of this book without the written permission of the publisher. CHAPTER 17 HANDOUT Mapping Chromosomes BLM 17.2.3 (cont’d) Part B: Using Linked Gene Notation In order to describe linked genes, fruit fly geneticists developed a different notation for genes. The normal allele—the allele that is nearly always found in natural populations—is called the wild type allele. The wild type allele is represented with a plus sign (+). The mutant allele for a gene is represented with lower-case italic letters. Linked genes are represented with a horizontal line or a slash. For example, the gene for purple eyes (pr) and the gene for vestigial wings (vg) are on chromosome number 2. prvg The homozygous recessive genotype is represented as or pr vg / pr vg to indicate that these prvg genes are on the same chromosome. The homozygous dominant genotype is represented as or + + / + +. Procedure 1. Create a chromosome map of three linked genes based on the research presented below. a) In fruit flies, the mutant gene d causes short legs and the mutant gene pr causes purple eyes. A geneticist performs the following cross: pr d / + + × pr d / pr d. She counts 1000 offspring and finds 391 wild type, 115 purple-eyed and normal-legged, 105 normal eyed and shortlegged, and 389 purple-eyed and short-legged. What is the map distance between the genes for leg length and eye colour? b) The same geneticist then performs the following cross: vg d / + + × vg d / vg d. She counts 1000 offspring and finds 350 wild type, 154 vestigial winged and normal-legged, 153 normal-winged and short-legged, and 343 vestigial-winged and short legged. What is the map distance between the genes for leg length and wing type? c) The recombination frequency for the gene for eye colour and the gene for wing type is 8.7%. What is the map distance between these two genes? Draw a chromosome map showing the relative distances between all three linked genes studied in Part B. Copyright © 2007, McGraw-Hill Ryerson Limited, a Subsidiary of the McGraw-Hill Companies. All rights reserved. This page may be reproduced for classroom use by the purchaser of this book without the written permission of the publisher. CHAPTER 17 HANDOUT Mapping Chromosomes BLM 17.2.3 (cont’d) Part C: Practice Questions Answer the following questions in your notebook. 1. Working in the famous “fly room” at Columbia University, you cross a homozygous recessive purple-eyed, vestigial-winged fruit fly (ppvv) with a heterozygous normal-eyed, normal-winged fly (PpVv). Both genes are found on chromosome 2. You discover the following percentages of flies in the F1 generation: 42 percent with normal eyes and normal wings; 46 percent with purple eyes and vestigial wings; 6 percent with normal eyes and vestigial wings; and 6 percent with purple eyes and normal wings. a) What is the expected outcome for a cross involving two linked genes? Why do you think your results differed from the expected outcome? b) Which of the offspring are recombinant types? c) Determine the recombination frequency for this cross. d) Based on your findings, how many map units apart are the genes for eye colour and wing type on chromosome 2? 2. In the same lab, your colleague is studying the genes for eye colour and body colour found on chromosome 2. She crosses a homozygous recessive purple-eyed, black-bodied fruit fly (ppgg) with a heterozygous normal-eyed, normal-coloured fly (PpGg). She counts 1000 offspring and finds 454 flies with normal eyes and normal body colour, 466 flies with purple eyes and black body colour, 42 flies with normal eyes and black body colour, and 38 flies with purple eyes and normal body colour. a) What are the recombination frequency and map distance between the two genes? b) From the data gathered by a third colleague, you know that the gene for wing type and the gene for body colour are 4 map units apart. Combining your colleagues’ data with your own findings in question 1, draw a chromosome map showing the linear arrangement of all three genes on chromosome 2. 3. In another experiment, you decide to cross a white-eyed female fruit fly (Xr Xr) with a red-eyed male (XR Y). a) Draw a Punnett square for this cross showing the F1 generation. b) What is the phenotype ratio for this cross? Copyright © 2007, McGraw-Hill Ryerson Limited, a Subsidiary of the McGraw-Hill Companies. All rights reserved. This page may be reproduced for classroom use by the purchaser of this book without the written permission of the publisher. CHAPTER 17 HANDOUT Mapping Chromosomes BLM 17.2.3 (cont’d) c) Which fly would you cross with the white-eyed male F1 offspring to get an F2 generation of all white-eyed flies? Use a Punnett square to support your answer. 4. In fruit flies, the following mutant genes have been identified: d causes short legs, vg causes vestigial wings, and pr causes purple eye colour. All three genes reside on chromosome 2. Using linked gene notation, write the genotypes of fruit flies that are a) homozygous recessive b) heterozygous c) homozygous dominant for these genes 5. A geneticist performs the following cross: vg d/+ + vg d/vg d. Of 1000 offspring, 385 are wild type, 102 have vestigial wings and normal legs, 108 have normal wings and short legs, and 405 have vestigial wings and short legs. a) What is the map distance between the genes for wing type and leg length? b) Previous data shows that the recombination frequency for the genes for wing type and eye colour is 10 percent, while that for leg length and eye colour is 31 percent. All three genes are found on chromosome 2. Draw a chromosome map showing the relative distances between these linked genes. Copyright © 2007, McGraw-Hill Ryerson Limited, a Subsidiary of the McGraw-Hill Companies. All rights reserved. This page may be reproduced for classroom use by the purchaser of this book without the written permission of the publisher.