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Download SUCCINYL-CoA SYNTHETASE from a prokaryote (Lot 140901b)
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SUCCINYL-CoA SYNTHETASE from a prokaryote (Lot 140901b) Recombinant E-SCOAS03/16 (EC 6.2.1.5) Succinate:CoA ligase (ADP-forming) PROPERTIES: 1. ELECTROPHORETIC PURITY: - Two bands (α and β subunits) on SDS-gel electrophoresis (MW ~ 30,843 and ~ 41,393) - One major bands on isoelectric focusing (pI ~ 5.9) 2. SPECIFIC ACTIVITY: 13 U/mg protein at pH 8.4 and 25°C. One Unit of succinyl-CoA synthetase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH under the following assay conditions: Glycylglycine buffer, pH 8.4 ATP Coenzyme A MgCl2 NADH PEP Succinic acid L-Lactate dehydrogenase Pyruvate kinase 3. 34 mM 1.2 mM 0.89 mM 3.4 mM 0.97 mM 2.6 mM 5.8 mM 3.4 U/mL 4.1 U/mL OTHER ACTIVITIES (as a percentage of succinyl-CoA synthetase activity): Enzyme Measured Substrate Activity, % Succinyl-CoA synthetase succinic acid 100 ATPase ATP < 0.002 NADH oxidase NADH < 0.001 4. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 8.4 and up to 25°C. 5. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM glycylglycine buffer, pH 8.4 containing 10 mM MgCl2. Swirl to mix the enzyme suspension immediately prior to use.