* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download BIOL 221_syllabus_part1_2010
Molecular cloning wikipedia , lookup
Human genome wikipedia , lookup
Genetic testing wikipedia , lookup
Synthetic biology wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Genomic library wikipedia , lookup
Biology and consumer behaviour wikipedia , lookup
Human genetic variation wikipedia , lookup
Point mutation wikipedia , lookup
Non-coding DNA wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Behavioural genetics wikipedia , lookup
Helitron (biology) wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Genome evolution wikipedia , lookup
Population genetics wikipedia , lookup
Genetic engineering wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Genome (book) wikipedia , lookup
Public health genomics wikipedia , lookup
Genome editing wikipedia , lookup
Designer baby wikipedia , lookup
History of genetic engineering wikipedia , lookup
BIOL 221 (Spring 2010) Molecular Biology and Genetics Lectures: Tuesdays and Thursdays from 9:00-10:30 am Location: Stiteler Hall B6 Instructors: Dr.Kimberly Gallagher ([email protected]) Office Hours: Tuesdays 12-1 in 121 Lynch Dr. Nancy Bonini ([email protected]) Office Hours: TBD Teaching Assistants: Dr. Stewart Gillmor ([email protected]) Problem Sessions: Weds 6-7 pm Office hours: Weds. 4-5 pm Dr. Katy Claiborn ([email protected]) Problem Sessions: Tues 5-6 pm in Leidy 10 Office hours: Thurs. 5:30-6:30 pm Goddard 101 Penn Blackboard Gateway: HYPERLINK "https://courseweb.upenn.edu/" https://courseweb.upenn.edu/ Description: This course will survey the discipline of Molecular Genetics. This will include two broad areas in molecular biology and genetics. Genetics - We will discuss both the application of Mendelian and molecular genetic techniques and principle to answering question in modern biology . We will learn how genes are passed from one generation to the next and how genetic analysis contributes to our understanding of other areas of biology including Biochemistry and Cell Biology. Lectures include discussion of experiments and techniques used in a variety of genetic systems including prokaryotes, eukaryotes and viruses. Molecular Biology - We will discuss, at the molecular level, processes such as DNA replication, transcription, and translation, and study the regulation of gene expression in both prokaryotes and eukaryotes. We will also discuss recombinant DNA technology and learn how powerful this technology has proven in elucidating the mechanisms of complex genetic control, and insights it has provided on the molecular basis for many human diseases and genomics. Textbook The text that we will be using this spring is Genetics: From Genes to Genomes (3rd Edition) by Hartwell et al. ISBN# 0077388046. The version of the text from the Penn bookstore is bundled with complete online/pdf access to the text and to the companion website. You will require access to the textbook for readings, study materials and homework. Occasionally you be required to read articles from the Encyclopedia of Life Sciences series published by Wiley. These article are in addition to readings from your textbook and often provide a more complex or in-depth look at particular concepts of processes. As these are written by experts in their field, they are often excellent. You are strongly encouraged not to ignore these. The free content articles are available through Wiley Publishing at http://www.els.net/. You can search by title or by author. Exams and Grading: This class is graded on a curve based upon the normalized mean scores for each exam. There are three exams scheduled during the semester and a cumulative Final Exam. Each exam, including the cumulative final, will be worth 100 points. Only your 3 highest normalized scores from the 4 total exams are included in the calculation of the final grade (i.e. we drop your lowest grade). This means that you may choose to not take the cumulative Final Exam, if you think you have done well on the three other exams. Since only three of the four exams are included in the calculation of your final grade, NO MAKE-UP EXAMS WILL BE GIVEN. You are strongly encouraged to take all four exams. Only students who take all four exams are eligible to receive an A+ in the class. Students who are enrolled in BIO 527 are required to take all four exams. Your grade will be based upon a total of 400 points 300 exam points plus 100 points from a term paper that will be assigned sometime during the middle of the semester. The grade on the term paper cannot be dropped. In addition throughout the semester there will be additional readings that you are strongly encouraged to complete. For all students, the overall class mean will be calculated after the lowest grade for each student is dropped. This means that the class average displayed in Blackboard is an underestimate of the actual class mean. Regrades and questions about grading of your exam will be considered only during a one-week interval after the day the exam has been returned to you and the exam key posted. These questions must be submitted in writing (typed- no handwritten requests will be accepted). It should be noted that when an exam is considered for regrading, all answers of the exam are subject to reconsideration. Exam submitted in pencil are not eligible for regrade. Homework: Throughout the semester you will be assigned homework problems (from the text, and questions of our own design). All homework assignments are optional, however you are srongly encouraged to complete all assignments and discuss any problems with the TAs during recitation sessions. We may occasionally request that you turn-in some of these homework problems for review. These will not be graded as such, but will be used as a class participation/extra credit score that we will take into consideration, if you are on the border between two grades. 221_2010_Syllabus_01_12_10.xls Page 1 BIOLOGY 221 SYLLABUS 2010 Date Lecture Topics Required Reading: Reading in italics may not Additional Recommended be explicitly covered in lecture, but are Readings (Required for 527 assumed knowledge. Students): Additional materials of interest Transmission Genetics 14-Jan Th Introduction to Genetic Analysis (KG) 1:1-13, 2:13-21 (Qs. 2: 3,4b,5,6,7,8,9,12) 19-Jan T Application of Mendel's Principles to Human Genetics (KG): Chromosomal Basis for Mendelian Inheritance (Oogenesis and Spermatogenesis); Introduction to Pedigrees 13:465-472;479-482 4:81-103, 2:30-37 21-Jan Th Analysis of Probability (KG) 26-Jan T 28-Jan Th 2-Feb T 2:21-30, Conditional Probability and the Reverend Thomas Bayes B. Everitt, Chance Rules, DOI: 10.1007/978-0-387-77415-2_9 Application of Mendels Principles to Model System Encyclopedia of Life Sciences Articles by Andrew Genetics and Disease (KG): The Significance of Wilkie: Dominance and Recessivity and by Dominance (Muller's Morphs); Introduction to Forward Burke Judd: Experimental Organisms Used in and Reverse Genetics. Genetics Beyond Mendel- Complex Dominance and Applications to Model System Genetics (KG): Incomplete and Co-Dominance; Penetrance and Expressivity; Complementation; Epistasis 3:47-72 Genetic Analysis in Practice (KG): Dissecting Yeast Secretion Peter Novick, Charles Field and Randy Schekman Identification of 23 Complementation Groups Required for Post-translational Events in the Yeast Secretory Pathway. and Lamason, et al. SLC24A5, a Putative Cation Exchanger, Affects Pigmentation in Zebrafish and Humans Gene Mapping 4-Feb Th Gene Identification: Linkage Analysis (part I) (KG) 9-Feb T Linkage Analysis (part II) (KG) 11-Feb T 16-Feb Th Experiments in Plant Hybridization (1865) by Gregor Mendel available in English at: http://www.mendelweb.org/Men del.html Encyclopedia of Life Sciences Encyclopedia of Life Sciences Article Article by Laura L Hall: Turner by Jean-Pierre Fryns: Monosomies Syndrome and by Michel Vekemans: Trisomy HJ Muller (1932) Further studies on the nature and causes of gene mutations Of particular interest are pages 231-252 beginning with "On the character of mutations." Huang and Sternberg Genetic Dissection of Developmental Pathways 5:123-142 Encyclopedia of Life Sciences Article by Alan F Wright: Genetic Variation: Polymorphisms and Mutations Exam I The Genetics of Bacteria and Viruses (KG) : Use of 15: 539-573 Microorganisms in Science and Society; Modes of DNA Encyclopedia of Life Sciences Article by Jill B transfer and mating. Keeney: Microorganisms: Applications in Molecular Biology Encyclopedia of Life Sciences Article by Sabine Hofmann and Matthias F Bauer Mitochondrial Disorders Molecular Biology of the Gene 18-Feb Th Cracking the code of life: DNA structure & replication (NB) 6:167-191 Watson & Crick (1953) Nature 171: 737-8; Lucia & Cairns (1969) Nature 224: 1164-6. 221_2010_Syllabus_01_12_10.xls 23-Feb T 25-Feb Th A messenger between the nucleus and cytoplasm: 8:255-275 RNA transcription & processing (NB) Translating the genetic code: Proteins & their 8:275-284 synthesis (NB) Yanofsky (2007) Cell 128: 8158; Crick et al. (1961) Nature 192: 1227-32. Gene Regulation 2-Mar T Gene regulation in prokaryotes: the Lac operon (NB) 8:282-284, 17:609-635 4-Mar Th Gene regulation in Eukaryotes (NB): Enhancers; chromatin remodeling; activation & inactivation of whole chromosomes 18:643-677, 13:465-482 Timko et al. (1985) Nature 318: 579-582; Lomvardas et al. (2006) Cell 126: 403-13. SPRING BREAK WEEK 16-Mar T Maintaining integrity of genome: Mutation & repair (NB) 7:207-244 18-Mar Th Variations in chromosome structure & number: Large scale chromosome changes (NB) 14:489-508, 516-526 23-Mar T Genomics 25-Mar Th 30-Mar T 1-Apr Th Zhang et al (2009) Annu Rev Genomics Hum Genet 10:45181. Exam II Techniques of DNA manipulation (NB): 9:310-339 Recombinant DNA; gel electrophoresis; blots; restriction maps; PCR; sequencing Aligning Genetic and DNA maps, and Genomics 10:351-383 (NB): Genome assembly; microarrays; bioinformatics Functional Genomics (NB) 11:391-425 Saiki et al (1988) Science 239: 487-491. Waterston et al. (2002) PNAS 99: 3712-6. Gene variation, evolution and complex interactions 6-Apr T 8-Apr 13-Apr 15-Apr 20-Apr Th T Th T 22-Apr Th 27-Apr 6-May Jumping genes (NB): Transposable elements in plants, animals and bacteria Developmental & behavioral genetics (KG) Developmental & behavioral genetics (KG) Population & evolutionary genetics (KG) Cancer, or the genome gone awry: oncogenes & tumor suppressors (NB) The Immune system: the components, antibodies & genome rearrangements, & HIV (NB) Exam III **CUMULATIVE FINAL** Scheduled for Thursday, May 6th, 12-2 pm 14:508-516, 22:811-813 Jones (2005) Cytogenet Genome Res 109: 90-103. 19:685-709 Murchison et al (2010) Science 327: 84-87. 8:270-271, 14: 492-494, 22:791-794,813-822 Page 2