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Course Offering in SPRING 2013 Genetic Engineering BSC 5425: 3 Credits Meera Nanjundan, Ph.D Department of Cell Biology, Microbiology, & Molecular Biology •Gene expression, Cloning, & Manipulation of Plasmids •Cell Culture •Transgenics •Mutagenesis •siRNA •Quantitative PCR •Generation of Antibodies •Use of Fluorescent Tags (i.e. GFP) •Protein Expression & Purification •High-throughput Technologies including: ÆArrays (DNA, RNA, Protein) ÆInCell Imaging ÆRetrovirus-based Protein Complementation Screens EGFP PI Merge Expression of a EGFP-tagged Nuclear Protein in a mammalian cell Array Profiling This course is open to all graduate students & upper level undergraduates. Entry into the course is through consent of the instructor. Cell Biology (PCB3023) & Genetics (PCB3063) are required . Cell or Genetics Laboratory is recommended. For more information, please contact Dr. Nanjundan: [email protected].