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SEXUAL REPRODUCTION Chapter 10 LET’S REVIEW! • The cell cycle is when a cell makes a copy of itself for: • Growth, Repair, Replacement • BODY (autosomal/somatic) cells reproduce by mitosis • some organisms do also, but we will talk about that later! CHROMOSOMES • Homologous Chromosomes: chromosomes that make a pair • They have the same length, same centromere position, and control the same inherited trait GAMETES • Gamete: Sex cell (sperm and egg) • Have half the # of chromosomes as compared to autosomal cells • Ensures that an organism has the same number of chromosomes from generation to generation • In humans, each gamete has 23 chromosomes • n = number of chromosomes in a gamete GAMETES • Haploid: n • Diploid: 2n (female n + male n) • When 2 human gametes combine through fertilization, 23 homologous chromosomes are formed • Meaning - Mom has 23 chromosomes, Dad has 23 chromosomes You have 46 chromosomes MEIOSIS • Process that creates gametes, occurs in reproductive structures • Cell reproduction that reduces the # of chromosomes • Mitosis MAINTAINS the number of chromosomes in the cell • Meiosis REDUCES it by half by splitting homologous chromosomes • 2n n INTERPHASE • Replication of DNA • Protein synthesis MEIOSIS I • Prophase I: Centrioles move to opposite poles, spindle fibers form and bind to sister chromatids at the centromere • Crossing Over (synapse): chromosome segments are exchanged between homologous chromosomes MEIOSIS I • Metaphase I: Homologous chromosomes line up at center of cell • Anaphase I: Homologous chromosomes separate and pulled to opposite ends of cell, chromosome # is reduced from 2n to n MEIOSIS I • Telophase I: Chromosomes reach poles • Each pole contains only one chromosome of the original homologous chromosomes MEIOSIS II • Prophase II: chromosomes condense • Metaphase II: HAPLOID (n) number of chromosomes line up at the equator MEIOSIS II • Anaphase II: sister chromatids are pulled apart • Telophase II: chromosomes reach poles and nuclear membranes and nuclei form MEIOSIS II Chromosomes DO NOT replicate between I and II end result is 4 haploid cells, each with n number of chromosomes MEIOSIS V MITOSIS SEXUAL VS. ASEXUAL REPRODUCTION* • Asexual • The organism inherits all of its chromosomes from one parent • Individual is genetically identical to its parent MEIOSIS IN MALES VS FEMALES • In females it is called oogenesis • Begins while in utero during the third trimester and stops until puberty. • Results in 1 healthy egg (ovium) and 3 polar bodies (cells with a nucleus and no cytoplasm) when completed • It is only complete after fertilization has occurred. MEIOSIS IN MALES VS FEMALES • In males it is called spermatogenesis • Doesn’t occur until puberty • Results in 4 haploid sperm cells. • Completed every ~74 days. MEIOSIS IN MALES VS FEMALES Completed Monthly GREGOR MENDEL • Genetics: the study of heredity • Heredity: the passing of traits from parent to offspring (INHERITANCE) • Gregor Mendel: Father of Genetics • Austrian Monk who experimented with garden peas in 1866, he noticed certain traits seemed to be passed from one generation to another GREGOR MENDEL • Mendel worked with peas, which self-fertilize • Some varieties always made green seeds, some always made yellow seeds, so he cross-pollinated the peas by hand • Parent Generation (P): 1st line of crosses • First Generation(F1): offspring of the parent generation • F2 Generation: second cross, using the F1 offspring ALLELES • He determined there must be TWO forms of a gene controlled by different factors • Alleles: alternative form of single gene • For example: height • Tall or short • Alleles are NOT genes, they are two different versions of one gene! ALLELES • Alleles are either dominant or recessive • Dominant: represented by a capital letter (T = tall) • This is the trait that is seen • Recessive: represented by a lowercase letter (t = short) • This trait is not seen, it is masked by the dominant allele ~ it’s there, just hidden! ALLELES • If the dominant allele is present, it will show up – DOMINANCE • There must be 2 recessive alleles in order to show up • We inherit an allele for a specific gene from each parent ALLELES • Homozygous: individual inherits 2 of the same allele • TT – homozygous dominant OR tt – homozygous recessive • Heterozygous: individual inherits 2 different alleles, one dominant and one recessive • Tt • Since the dominant allele is present, it will show GENOTYPE & PHENOTYPE • Genotype: organism’s allele pairs • Heterozygous, homozygous dominant, or homozygous recessive • Phenotype: observable appearance of genes PUTTING IT ALL TOGETHER… GENOTYPE (HETEROZYGOUS) = ALLELE ALLELE PHENOTYPE LAW OF SEGREGATION • Two alleles for a trait separate during meiosis • Each gamete will have a different allele • They will be reunited during fertilization LAW OF INDEPENDENT ASSORTMENT • When gametes are made during meiosis each only gets one copy of a gene • This is random; the inheritance of one gene does not influence the inheritance of another gene; they are independent • Every person with brown hair doesn’t have brown eyes • Some genes are inherited together (LINKED) because the genes are very close to each other on the chromosome. • people with red hair are also fair-skinned. PUNNETT SQUARE • Used to predict the possible offspring between two known genotypes • Monohybrid: crossing one trait at a time • Dihybrid: crossing two traits PUNNETT SQUARE Parent 1- Pure Short Parent 2 – Pure Tall F1 Generation TONGUE ROLLING • Dominant Trait ~ T • 2 parents are heterozygous (Tt) for the trait • What possible phenotypes will their children have? T t T TT Tt t Tt tt Tongue roller Tongue roller Tongue roller Non-tongue roller PUNNETT SQUARES • Determining ratios Chapter 10 Sexual Reproduction and Genetics 10.2 Mendelian Genetics Punnett Square— Dihybrid Cross Four types of alleles from the male gametes and four types of alleles from the female gametes can be produced. The resulting phenotypic ratio is 9:3:3:1. PUNNETT SQUARE Chapter 10 Sexual Reproduction and Genetics 10.3 Gene Linkage and Polyploidy Genetic Recombination The new combination of genes produced by crossing over and independent assortment Combinations of genes due to independent assortment can be calculated using the formula 2n, where n is the number of chromosome pairs. Chapter 10 Sexual Reproduction and Genetics 10.3 Gene Linkage and Polyploidy Gene Linkage The linkage of genes on a chromosome results in an exception to Mendel’s law of independent assortment because linked genes usually do not segregate independently. Chapter 10 Sexual Reproduction and Genetics 10.3 Gene Linkage and Polyploidy Polyploidy Polyploidy is the occurrence of one or more extraof all sets chromosomes in an organism. A triploid organism, for instance, would be designated 3n, which means that it has three complete sets of chromosomes.