Download Health assessment of freshwater mussels using metabolomics

Health assessment of
freshwater mussels using
metabolomics
I E VA ROZN E RE
P H. D. CA N DI DATE
T HE OHI O STATE U N I V ERSITY
Problem
Freshwater mussels are the most endangered faunal group in NA.
Relocation and captivity result in increased mortality and reduced
growth rates.
Limited knowledge of mussel health
Health assessment
relocation
↓
captivity
↓
food limitation
Metabolomics
(Goodacre 2005)
Food limitation experiment
Objective: Assess the metabolic changes in freshwater mussels
brought into captivity and subjected to food limitation
Hypothesis: Freshwater mussels held in captivity experience
nutritional deficiency which will be exhibited by changes in
metabolites associated with energy-producing pathways.
Muskingum
Captivity
Food limitation
Metabolomics
↓
Carbohydrate
Lipid
Amino acid
Nucleic acid
Carbohydrates
glucose
maltose
glycogen
pyruvate
lactate
acetyl-CoA
acetylcarnitine
citrate
oxaloacetate
cis-aconitate
NADH
malate
isocitrate
NADH
α-ketoglutarate
fumarate
FADH2
succinate
succinyl-CoA
NADH
(McCue 2010)
Fatty acids
Amino acids
Nucleotide metabolites
Conclusions
Carbohydrates reduced, except glucose (gluconeogenesis?)
Switch to fat as energy source
Decrease in amino acids and nucleotides
Acknowledgements:
John Navarro
Doug Warmolts
Mike Brittsan
Clarissa Bey
Trisha Gibson
Jacqualyn Halmbacher
Mac Albin
EnviroScience
Brandon Sinn
Funded by:
Ohio Department of
Natural Resources
Ohio Biodiversity
Conservation Partnership
Columbus Zoo &
Aquarium