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C383 Study Guide for Exam 3
Spring 2016
Basic Information
 Exam 3: Thursday, April 14, 7:15-9:15PM in Chemistry 122
 Arrive early for assigned seats
 Bring your student ID. Failure to do so will result in getting your exam back later.
 You may use a NON-PROGRAMMABLE calculator.
 All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
Exam Content: The exam will cover chapters 15-21 and 24-26. (Chapter 26 will be highly
abbreviated.) All material covered in class, homework, and discussion sections could be on the
exam. Some major topics include:
Basics of metabolism: qualitative and quantitative free energy, coupled reactions, phosphoryl
group transfer potential, activated carriers
Glycolysis: Structures of all intermediates, names of all intermediates, names of regulated
enzymes, role of cofactors
Gluconeogenesis: Structures of all intermediates, names of all intermediates, names of regulated
enzymes, role of cofactors
Pyruvate Dehydrogenase Complex: Overall reaction, purpose of cofactors, metabolic purpose
Citric acid Cycle: Structures of all intermediates, names of all intermediates, names of regulated
enzymes, mechanisms presented in slides only
Electron transport chain: know complexes by number, mobile carriers, protons pumped
Oxidative phosphorylation: evidence for chemiosmotic hypothesis, role of protonmotive force,
structure of ATP synthase, binding-change mechanism, purpose of shuttles, P/O ratio
Glycogen metabolism: reciprocal regulation, structure of glycogen affects formation/degradation,
regulation of phosphorylase in muscle and liver, regulation of glycogen synthase and role of PP1
Pentose phosphate pathway: production of NADPH and ribose; recycling of 5-c sugars to 3- and
6-c sugars
General topics:
 Know STAGES and OVERALL PURPOSES of each pathway we have learned
 Know ATP/NADH production/utilization steps and overall accounting
 Compartmentalization of pathways
 Know regulatory steps; understand why regulatory scheme makes sense in big picture
 Determine free energy changes of reactions qualitatively and quantitatively
 Physiology/pathology from slides
Exam Format: The exam is out of 100 points and will follow the same format as precious exams.
There will be conceptual and quantitative problems. Thermodynamic equations and constants
will be given. Structures of cofactors will be given if necessary. Although there might be bonus
opportunities, the most points you can get on this exam is 100.