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A Platform for Cluster Analysis of Next-Generation Sequencing RNA-Seq Data The purpose of gene expression data clustering analysis is clustered genes with the same or similar functions to help explore the gene function and regulatory network. The past is mainly based on microarray gene expression data, in recent years due to the development of next-generation sequencing technology. Transcriptome sequencing (RNA-Seq) data have many advantages over conventional microarray technology to obtain the gene expression data.So ,many clustering analysis use the gene expression data generated by the RNA-Seq data, among them, the probability distribution model will get better clustering results.Besides,functional information or knowledge (for example, gene semantic similarity) of genes involved in the clustering is also improved for gene function correlation of the grouping results. Therefore, the development of a set of RNA-Seq clustering analysis platform based on probabilistic model method and gene semantic similarity is very helpful to study the clustering analysis of the second generation transcript sequence data.