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Abstract
Transitional cell bladder cancer is common in the UK. And ranked the sixth among malignant
tumors. Chemotherapy resistance is a major problem since it can lead to failure of
chemotherapy as a result of single or multiple drug resistance. The gens known as heat shock
proteins HSP20, HSP27, HSP60, HSP70 and HSP90 have an important roles in the development
and chemotherapy drug resistant in bladder cancer. In this steady, HSP27 was used as the
control gene. Since many genes interact with HSP27 directly or indirectly, FSCNI, PRKCD,
MAPK11, ESR1, and Beta catenin.
Using conventional PCR and QPCR the presence of HSP27 and its related genes as well as their
level of expression were detected.
It has been found that all genes are expressed at higher levels in MGH_U1 (transitional cell
carcinoma), particularly ESRI and MAPK11, their expression in MGH_U1 was 6 and 3 folds
compared to SCaBER, except the control gene HSP27 which was expressed in SCaBER
(squamouse cell carcinoma).
The higher level of ESR1 in both cell lines especially MGH_U1 indicate their possible
susceptibility to treatment to tamoxifen.
Differences in HSP27 in both cell lines indicate possible differences in apoptosis since this gene
has an important role in regulation apoptosis.
Further studies and evaluation of these genes are now being performed.