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Chapter 9 Harvesting Energy: Glycolysis Fermentation Cellular Respiration Introduction and Vocabulary: Metabolism: Cells can metabolize a variety of organic compounds,but we will concentrate on the metabolism of glucose because it is the most common form of metabolism NAD+: Coenzyme used in reactons, can accept electrons and becomes NADH Aerobic: with oxygen Anaerobic: without oxygen Different Stages of Glucose Metabolism: 1. Glycolysis • • • Does not require oxygen Occurs in Cytoplasm of the cell Produces pyruvate, 2 ATP, and 2 NADH Different Stages of Glucose Metabolism: 2. Fermentation • Without oxygen • Anaerobic • Pyruvate is converted into lactate or ethanol • No ATP Produced • Occurs in cytoplasm of cells Different Stages of Glucose Metabolism: 3. Cellular Respiration: Includes glycolysis, Krebs cycle, and electron transport chain • • • • Presence of oxygen Aerobic Pyruvate converted into CO2 and H2O, and 36 to 38 ATP are produced Occurs in the mitochondria of cells How is The Energy of Glucose Captured During Glycolysis? Glycolysis: means to “break apart” • • • • Glucose is broken down with or without oxygen in the cytoplasm into pyruvate One Glucose is cleaved into two pyruvate Produces little energy Two ATP and Two NADH produced Glycolysis Has Two Steps: a). Glucose Activation b). Energy Harvesting Stage a).Glucose Activation: • • Before glucose is broken down, it is activated. This takes energy (2 ATP) This reaction cost 2 ATP molecules b).Energy Harvesting Steps: • • • • 1 Glucose splits into 2 G3P (3 carbon molecule) G3P molecule converts to 2 pyruvate 2 ATP are generated for each G3P to total 4 ATP, but you only get 2 ATP since you used 2 ATP to activate glucose 2 NADH produced, NADH is an electron carrier, NAD+ is an electron acceptor Note: if oxygen is available, cellular respiration will occur in the mitochondria of the cell, but there is no available oxygen, fermentation will take place in the cytoplasm. Absence of oxygen • Production of ethanol or lactate • This is called fermentation • No ATP produced • Occurs in cytoplasm Two Types of Fermentation a). Conversion of pyruvate into lactate (lactic acid) b).Conversion of pyruvate into Co2 + Ethanol a). Fermentation to Lactate: (Muscles) • • • • • • Occurs in cytoplasm of muscles after vigorous exercise, sprinting fast, anaerobic activity Build up of Lactic acid Not enough oxygen to lungs, blood, and muscles to allow for cellular respiration Glycolysis does not stop Without oxygen, muscle cells ferment pyruvate to lactate or lactic acid If you stop the anaerobic exercise or slow down, and as you breath, oxygen becomes available. Fermentation Continued: • • Lactate converts back to pyruvate, this happens in the liver Pyruvate then in presence of oxygen can be broken down into CO2 + H2O by cellular respiration 2 ADP+ 2 Glycolysis 2 NAD 2 NAD Glucose 2 Pyruvic acid + 2 H 2 Lactic acid (a) Lactic acid fermentation Figure 6.15a b). Fermentation to Alcohol ( microorganisms) • • • • • • Many microorganisms use this process Anaerobic conditions Produces ethanol and CO2 from pyruvate Yeast (microorganism), fermented fruits and grains into alcoholic beverages Champaign is bottled while the yeast are still alive so it traps both ethanol and CO2 and gives the cork a pop when opened Bakers yeast produces CO2, that is what makes the bread rise, the ethanol evaporates while cooking in the over THE END OF HARVESTING ENERGY Part 1 Glycolysis and Fermentation