Download GBE 335 MOLECULAR GENETICS

Course Code : GBE 335
Course Title : MOLECULAR GENETICS
Level : Undergraduate
Year : II,III
Status : Elective
Hours/Week : 5
Semester : IV,V,VI
ECTS Credits : 5
Total Hours : 75
Instructor :
COURSE DESCRIPTION
Nucleic acids structure, chromosomal DNA, DNA replication, DNA repair, genetic
recombination, control of gene expression, RNA and protein synthesis, viruses, plasmids,
transposable genetic elements, recombinant DNA technology.
COURSE OBJECTIVES
Understand basic terms Be able to use fundamental DNA replication, transcription, and
translation terms correctly Understand the mechanisms of DNA replication, transcription, and
translation Describe the mechanisms in general for prokaryotes and eukaryotes Compare and
contrast the mechanisms in prokaryotes and eukaryotes Describe the consequences of
defects in these mechanisms Describe the regulation of these processes in prokaryotes and
eukaryotes Understand the techniques included in the molecular biology “toolkit” Describe the
techniques for nucleic acid and protein analysis Describe experiments to address biological
questions and select the appropriate techniques to use for these experiments Be able to
communicate clearly: written communication and oral communication Be able to collaborate
with others (team-working) Be able to evaluate experiments critically (reading, interpreting,
and evaluating research articles)
COURSE CONTENTS

Mendel/ DNA as genetic information Central Dogma

Techniques in Molecular Biology I: Nucleic Acids

Techniques in Molecular Biology: Proteins/Nucleic Acid-Protein Interactions

Structures of DNA and RNA

Chromosomes: general properties higher order organization

DNA replication I: Mechanism Initiation, completion, telomerase

DNA mutation and repair, Homologous recombination

Transcription I: prokaryotes, eukaryotes, RNA processing : splicing

RNA processing : additional mechanisms. Translation I: components Translation II:
initiation Translation III: elongation

Translation IV: termination/regulation

Gene regulation in prokaryotes I: operon control, phage lambda

Gene regulation in eukaryotes I: general mechanisms ,epigenetics, silencing

Regulatory RNAs I: Bacteria, human, RNAi

Gene regulation during development I: differential expression
TEACHING/ASSESSMENT
Description
Teaching Methods
1. Interactive
2. Discussions and group works
3. Tutorials and Labs
The goal is to maximize student’s
participation in all teaching methods.
Description(%)
Student Assessment
Methods
Learning Outcomes
Quizzes
Homework
Project
Midterm Examination
Final Examination



10%
10%
20%
20%
40%
Actively participate in courses and begin to take responsibility for learning
Begin to work effectively as part of a team, developing interpersonal, organisational
and problem-solving skills within a managed environment, exercising some personal
responsibility.
Present information in oral, written or graphic forms in order to communicate
effectively with peers and tutors.
Language of Instruction
Textbook(s)
English
1)
2)
Molecular Biology of the Gene, by James D. Watson, (6th Edition, 2007).
Human Molecular Genetics, Third Edition
Tom Strachan, Andrew Read