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Name: ________________________________ Biology, Mr. Lo Period: _______ Date: _____________ Mitosis Lab Purpose 1. Locate cells in prepared onion root slides that are in the process of mitosis. 2. Using your class diagrams, identify and draw cells that are indifferent stages of mitosis. Materials: microscope, prepared slides of onion root tip Procedure and Questions 1. Under low power, locate with a microscope the region of rapidly dividing cells on the prepared slide of onion root tip as shown in the picture below. After locating the cells, switch to high power. 2. Locate cells that appear to be in various stages of mitosis. Use the figure below as a guide of what you should see. 3. Using the figure below, label one clear example of each stage of mitosis: interphase, prophase, metaphase, anaphase, and telophase/cytokinesis. 1 Name: ________________________________ Period: _______ Date: _____________ Biology, Mr. Lo 4. Each microscope has been setup so that you can see as many stages of mitosis in one view. Using a pencil, draw 4 cells undergoing different stages of mitosis. Next, write the stage of mitosis the cell is going through as well as a short description explaining your answer. Stage: _______________________________ Stage: _______________________________ Stage: _______________________________ Stage: _______________________________ 2 Name: ________________________________ Biology, Mr. Lo Period: _______ Date: _____________ Post-Lab Questions 1. Why do you think we look at the onion root cell for examples of cells going through the different stages of mitosis? 2. Why does a cell undergo mitosis? 3. When a cell is in interphase… a. Is the DNA in the form of chromatin or chromosomes? b. Is the nuclear membrane present? c. In what sub-stage is the DNA replicated? 4. At the end of prophase… a. Is the DNA in the form of chromatin or chromosomes? b. Is the nuclear membrane present? 5. At the end of metaphase… a. Where is the DNA located in the cell? b. What brought the DNA to this location? c. Where do these objects attach to the DNA? 6. When a cell is in anaphase… a. What happens to the DNA? 7. At the end of telophase… a. Is the nuclear membrane present? b. Is the DNA in the form of chromatin or chromosomes? 8. When a cell finished cytokinesis… a. Is the nuclear membrane present? b. Is the DNA in the form of chromatin or chromosomes? c. How many cells are present? 3 Name: ________________________________ Biology, Mr. Lo Period: _______ Date: _____________ Cell Cycle Activity In this online activity, you will look at 36 pictures of actual onion root cells in various stages of the cell cycle. You and a partner will classify each cell based on its stage of mitosis. Based on the number of cells found in each phase, you can approximate the amount of time cells spends in each stage. Procedure 1. Go to http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html. 2. Click on “Next” and review the information and provided diagrams. 3. Click on “Next” through the next two sections. 4. Identify the stages of mitosis for each cell. 5. Count the number of cells in each stage of mitosis and complete the following chart. Interphase Prophase Metaphase Anaphase Telophase Total # of Cells 36 % of Cells 100% Amount of Time in Each Stage 24 hours 6. To calculate the percentage of cells in each stage, divide the # of cells in each stage by 36 and multiply by 100%. Your answer should be accurate to 1 decimal place. % of Cells # of cells 5 X 100% X 100% 13.9% Total # of cells 36 7. To calculate the amount of time spent in each stage… a. Convert the % of cells into a decimal (13.9% = .139). b. Multiply this percentage by 24. This will give you the amount of hours spent in each stage. Answers should be accurate to 1 decimal place. .139 X 24 hours 3.3 hours Post-Lab Questions 1. In which stage do cells spend the… a. longest amount of time? b. least amount of time? 2. Explain these differences. 4