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Download Full DNA Polymerase Enzyme Mix
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Certificate of Analysis In the interest of conserving resources, we are no longer shipping manuals with products. Please visit www.clontech.com/manuals to obtain an electronic version. PRODUCT: Full DNA Polymerase Enzyme Mix CATALOG No. 639233 639234 AMOUNT 500 units 5,000 units SHELF LIFE 1 year from date of receipt under proper storage conditions. DESCRIPTION T h e Ta q Fu l l D N A Po l y m e r a s e i s a full-length recombinant version of the Thermus aquaticus (Taq) strain YT1 DNA polymerase. It is a broad range enzyme suitable for a variety of general PCR applications. One unit of enzyme incorporates 10 nmols of dNTPs into acid-insoluble material within 30 min at 74°C. The Taq Full DNA Polymerase Enzyme Mix contains the Taq Full DNA Polymerase as well as optimized PCR Buffer containing 20 mM MgCl2. SHIPPING CONDITIONS Blue ice (4°C) or dry ice (–70°C). PACKAGE CONTENTS 639233 (500 units) LOT NUMBER Specified on product label. STORAGE CONDITIONS Store all components at –20°C. • 100 µl Taq Full DNA Polymerase Mix (5 units/µl) • 3 x 1.25 ml 10X Taq Full PCR Buffer • User Manual (PT3820-1) • Protocol-at-a-Glance (PT3820-2) 639234 (5,000 units) FOR RESEARCH USE ONLY QUALITY CONTROL United States/Canada 800.662.2566 See back of page. Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Ave. Mountain View, CA 94043 Technical Support (US) E-mail: [email protected] www.clontech.com (PA053836) • 1 ml Taq Full DNA Polymerase Mix (5 units/µl) • 30 ml 10X Taq Full PCR Buffer • User Manual (PT3820-1) • Protocol-at-a-Glance (PT3820-2) Taq Full DNA Polymerase Enzyme Mix Cat. Nos. 639233 & 639234 RAW MATERIAL Quality control Purified Taq Full DNA polymerase was tested for enzymatic activity and PCR performance. Endonuclease and DNA contamination assays were also performed. functional Quality control Amplification from a genomic DNA template: Taq Full DNA polymerase was tested in a 50 µl PCR reaction using 1 µl (100 ng) of calf thymus genomic DNA as a template and control primers specific for a 407 bp fragment of the bovine pancreatic trypsin inhibitor (BPTI) gene (0.4 µM each). Conditions were set at: 1 cycle 94°C, 3 min 25 cycles 94°C, 30 sec 68°C, 1 min 1 cycle 68°C, 5 min 5 µl of PCR product was run on a 1.2% TAE/agarose gel to confirm the presence of a 407 bp band with minimal background. PCR product concentration was measured by fluorometry. The yield was determined to be >5 ng/µl. Notice to Purchaser Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of Clontech Laboratories, Inc. NOTICE TO PURCHASER: LIMITED LICENSE PCR: NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered 6,127,155. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim (such as method claims in U.S. Patents Nos. 5,994,056 and 6,171,785) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is hereby conveyed by the purchase of this product expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Clontech, the Clontech logo and all other trademarks are the property of Clontech Laboratories, Inc., unless noted otherwise. Clontech is a Takara Bio Company. ©2010 Clontech Laboratories, Inc. This document has been reviewed and approved by the Clontech Quality Assurance Department. PA053836 2 www.clontech.com Clontech Laboratories, Inc.