Download Mouse anti-GPCR-K2 Mouse anti-β-Adrenergic Receptor Kinase 1

Qty: 100 µg/200 µl
Mouse anti-GPCR-K2
Catalog No. 35-0100Z
Lot No.
Mouse anti-β-Adrenergic Receptor Kinase 1
(also called: G Protein Coupled Receptor Kinase 2)
FORM
This monoclonal antibody is supplied as a 200 µl aliquot at a concentration of 0.5 mg/ml in PBS, pH 7.4, containing 0.1%
sodium azide. This antibody is highly purified from mouse ascites by protein A chromatography.
CLONE: 5D5
ISOTYPE: Mouse IgG2b
IMMUNOGEN
Recombinant β-adrenergic receptor kinase.
SPECIFICITY
This antibody reacts with mammalian β-adrenergic receptor kinase.
REACTIVITY
Reactivity confirmed with human and rat.
Sample
Human
Rat
Immunogen
Immunoprecipitation
(native)
ND
++
NA
Immunoprecipitation
(Kin. Act.)
ND
++
NA
Western
Blotting
Immunofluoresence
ELISA
IHC
(Frozen)
IHC
++
++
+++
+++
+++
NA
+++
+++
+++
+++
+++
NA
+++
+++
NA
(Excellent +++, Good++, Poor +, No reactivity 0, Not applicable NA, Not determined ND)
USAGE
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be
affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method,
temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended starting points for this product.
Immunoprecipitation:
ELISA:
Western Blotting:
Immunofluoresence:
IHC:
10ug/IP reaction
0.1-1 ug/ml
1-5 ug/ml
3-10 ug/ml
10 ug/ml
STORAGE
Store at 2-8°C for up to one month. Store at –20°C for long term storage. Avoid repeated freezing and thawing.
(cont’d)
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI350100Z
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website,
www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
(35-0100Z cont’d)
BACKGROUND
G protein-coupled receptor kinases, a more recently discovered family, have the unique features of phosphorylating Gprotein coupled receptors only when they are in their active or stimulated conformations. The range of signals acting through
G protein-coupled receptors includes numerous neurotransmitters, chemoattractants, hormones, cytokines, and sensory
stimuli such as photons or odorants. Structurally, the G-protein coupled receptor kinases show significant amino acid
similarity to other kinases only in the centrally located catalytic domain of about 240 amino acid residues. Rapid
phosphorylation of many G protein coupled receptors accompanies stimulus-driven desensitization. There are two types of
kinases known to mediate these modifications: second messenger kinases and novel G protein-coupled receptor kinases.
G protein-coupled receptor kinase family members include rhodopsin kinase, two isoforms of beta-adrenergic receptor
kinase (betaARK), a gene cloned from the Huntington’s disease locus on chromosome 4, GRK5, and several homologs from
Drosophila. BetaARK1 is widely distributed throughout the mammalian central nervous system, especially at synapses,
suggesting broader roles in regulating a wide variety of neurotransmitter receptors. BetaARK1 was identified as a kinase
that phosphorylates betaARs in an agonist-dependent manner. Beta ARK1 has properties similar to the rhodopsin kinase in
that BetaARK is also insensitive to cAMP, cGMP, and Ca2+ and does not phosphorylate histone. BetaARK is capable of
phosphorylating photoexcited rhodopsin, although the betaAR is a better substrate for betaARK than rhodopsin is. Both
rhodopsin kinase and betaARK are inhibited by salts such as 0.1M NaCl, low concentrations of Zn (0.1-1mM), detergents
such as digitonin, and polyanions such as dextransulfate and polyaspartic acid. Both kinases use ATP as the preferred
substrate over GTP. In the case of betaARK, the kinase interacts with G protein beta-gamma subunits as well as with
receptors, and the ternary complex of agonist bound receptor, kinase, and beta-gamma subunits is considered to be an
active form for the phosphorylation of receptors. Multiple independent lines of evidence indicate that the phosphorylation of
receptors by G-protein coupled receptor kinases in vivo regulates their function.
REFERENCES
1. Robert J. Lefkowitz. G Protein-Coupled Receptor Kinases. Cell 74: 409-412, 1993.
2. Tatsuya Haga, Kazuko Haga, and Kimihiko Kameyama. G Protein-Coupled Receptor Kinases. Journal of
Neurochemistry 63(2): 400-412, 1994.
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Zymed and ZyMAX™ are trademarks of Zymed Laboratories Inc. Cy™ is a trademark of Amersham Life Sciences, Inc. Sepharose is a
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®
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For Research Use Only
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI350100Z
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website,
www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these
products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.