Download Bioassay-Validated Recombinant Cytokines

Bioassay-Validated
Recombinant Cytokines
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Mouse IL-33 ������������������������������������������������� 439
Mouse IL-34 ������������������������������������������������� 439
Mouse Isthmin1 ����������������������������������������� 440
Mouse MCP-1 (CCL2) �������������������������������� 440
Mouse SCF ��������������������������������������������������� 440
Mouse TACI-Fc Chimera ������������������������� 441
Mouse TNF-α ����������������������������������������������� 441
Mouse TRANCE (RANKL) ������������������������� 441
Mouse VEGF120 ������������������������������������������� 442
Rat IL-2 ����������������������������������������������������������� 442
Rat IL-6 ����������������������������������������������������������� 442
Rat TNF-α ����������������������������������������������������� 443
Human CXCL9 (MIG) ��������������������������������� 443
Human FGF-basic/145 aa ����������������������� 443
Human G-CSF ��������������������������������������������� 443
Human GM-CSF ����������������������������������������� 444
Human IFN-γ ����������������������������������������������� 444
Human IL-1α ������������������������������������������������� 444
Human IL-1β ������������������������������������������������� 444
Human IL-2 ��������������������������������������������������� 445
Human IL-3 ��������������������������������������������������� 445
Human IL-4 ��������������������������������������������������� 445
Human IL-5 ��������������������������������������������������� 446
Human IL-6 ��������������������������������������������������� 446
biolegend.com • [email protected]
Human IL-7 ��������������������������������������������������� 446
Human IL-8 ��������������������������������������������������� 446
Human IL-10 ������������������������������������������������� 447
Human IL-12 (p70) ������������������������������������� 447
Human IL-13 ������������������������������������������������� 448
Human IL-15 ������������������������������������������������� 448
Human IL-17A ��������������������������������������������� 448
Human IL-17A/F Heterodimer ��������������� 448
Human IL-17F ����������������������������������������������� 449
Human IL-22 ������������������������������������������������ 449
Human IL-23 ������������������������������������������������ 449
Human IL-27 ������������������������������������������������� 450
Human IL-34 ����������������������������������������������� 450
Human MCP-1 (CCL2) ������������������������������� 450
Human PDGF-BB ��������������������������������������� 451
Human RANTES (CCL5) ��������������������������� 451
Human SDF-1α (CXCL12) ������������������������� 451
Human TGF-β1 ������������������������������������������� 452
Human TNF-α ��������������������������������������������� 452
Human TNF-β ��������������������������������������������� 453
Human TWEAK (CD255) ��������������������������� 453
Human VEGF-165 ��������������������������������������� 453
Bioassay-Validated Recombinant Cytokines
Mouse CXCL9 (MIG) ���������������������������������
Mouse FGF-basic ���������������������������������������
Mouse GM-CSF �������������������������������������������
Mouse IFN-γ �����������������������������������������������
Mouse IL-1α �������������������������������������������������
Mouse IL-1β �������������������������������������������������
Mouse IL-2 ���������������������������������������������������
Mouse IL-3 ���������������������������������������������������
Mouse IL-4 ���������������������������������������������������
Mouse IL-5 ���������������������������������������������������
Mouse IL-6 ���������������������������������������������������
Mouse IL-7 ���������������������������������������������������
Mouse IL-10 �������������������������������������������������
Mouse IL-12 (p70) ���������������������������������������
Mouse IL-12/IL-23 p40 (homodimer) ���
Mouse IL-13 �������������������������������������������������
Mouse IL-15 �������������������������������������������������
Mouse IL-17A �����������������������������������������������
Mouse IL-17A/F Heterodimer �����������������
Mouse IL-17F �����������������������������������������������
Mouse IL-22 �������������������������������������������������
Mouse IL-23 �������������������������������������������������
Mouse IL-25 (IL-17E) �����������������������������������
Mouse IL-27 �������������������������������������������������
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Mouse CXCL9 (MIG)
Other Name: SCYB9
CXCL9 is an inflammatory chemokine initially identified by differential screening of a cDNA library from limphokine-activated
macrophages. CXCL9 is a CXC chemokine and member of the nonELR (lacking a Glu-Leu-Arg motif in the N-terminal region) CXC
chemokine family. CXCL9 shares the CXCR3 receptor with CXCL10
and CXCL11, and these ligands differ in their receptor-binding and
activating properties; CXCL11 is more potent than CXCL10, and
CXCL10 is more potent than CXCL9.
Please request a quote for amounts greater than 500 µg.
Mouse CXCL9, amino acids
Thr22 - Thr126 (Accession #
NM_008599), was expressed in
E. coli, and purified to greater
than 98% purity.
T cells chemoattracted
by mouse CXCL9
Bioactive Protein: Bioactivity was measured by its property to chemoattract human T cells activated with PHA and IL-2
in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100
µg and at higher concentrations for sizes of 500 µg and higher.
Bioactive Protein (Carrier-Free)
578202 578204 578206 578208
25 μg 100 μg 500 μg
10 μg
$225
$125
$595
$2000
Please request a quote for amounts greater than 500 µg.
Other Names: CSF-α, Pluripoietin-α, Eo-CSF, BPA
GM-CSF (granulocyte/macrophage-colony stimulating factor) is
a hematopoietic factor that is produced by T cells, macrophages,
fibroblasts and endothelial cells. This multifunctional cytokine
stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor
for granulocytic and monocytic cells.
Recombinant Mouse GM-CSF
Applications: Bioassay
Recombinant Mouse GM-CSF
(Ala 18–Lys 141, Accession
#NM_009969) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (450nm)
ng/mL
Bioactive Protein (Carrier-Free)
579602 579604 579606 579608
25 μg 100 μg 500 μg
10 μg
$225
$125
$550
$1600
Mouse GM-CSF
Application: Bioassay
Chemotaxis Index
Bioassay-Validated Recombinant Cytokines
Recombinant Mouse CXCL9 (MIG)
Bioactive Protein: This FGF-basic protein is biologically active
with an ED50 of 0.3 to 2 ng/mL, corresponding to a specific
activity of 0.5 to 3.3 × 106 units/mg, as determined by the dose
dependent stimulation of 3T3 cell proliferation. Endotoxin is
less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes
10–100 µg and at higher concentrations for sizes of 500 µg and
above.
Bioactive Protein: This
GM-CSF protein is biologically
MC/9 cell proliferation
active with an ED50 of 0.05 ng/
induced by mouse GM-CSF.
mL (2 × 107 units/mg). Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL
for sizes 10–100 µg and at higher concentrations for sizes of 500
µg and above.
ng/mL
Mouse FGF-basic
Other names: Fibroblast growth factor 2, Fgf-2, Fgfb, bFGF
FGF-basic (FGFb) is a member of the fibroblast growth factor (FGF)
family which includes 23 members. It is also known as fibroblast
growth factor 2, Fgf-2, Fgfb, and bFGF. FGFb is expressed in almost
all tissues and plays an important role in a variety of normal and
pathological processes, including development, wound healing,
and neoplastic transformation. FGFb is mitogenic for many cell
types, both epithelial and mesenchymal. It shows potent angiogenic activity and has been implicated in tumor angiogenesis.
FGFb also significantly promoted the proliferation of adipose-derived mesenchymal cells (AMC) and enhanced chondrogenesis in
three-dimensional micromass culture. FGFb binds to a family of
four distinct, high affinity tyrosine kinase receptors, designated
FGFR-1 to -4. In addition, FGFb binds to the extracellular matrix
and heparan sulfate (HS), which is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to
explain, at the molecular level, how HS/heparin enables FGF and
FGF receptor (FGFR) to assemble into a functional dimer on the
cell surface, although there is controversy regarding the exact
manner by which this occurs.
Bioactive Protein (Carrier-Free)
576302 576304 576306 576308
25 μg 100 μg 500 μg
10 μg
$225
$125
$700
$1700
Please request a quote for amounts greater than 500 µg.
Mouse IFN-γ
Other Names: Interferon-γ, Immune interferon,
Type II interferon, T cell interferon, Macrophageactivating factor, MAF
Interferon-γ (IFN-γ) is a potent multifunctional cytokine which
is secreted primarily by activated NK cells and T cells. Originally
characterized based on anti-viral activities, IFN-γ also exerts antiproliferative, immunoregulatory, and proinflammatory activities.
IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Recombinant Mouse IFN-γ
Recombinant Mouse FGF-basic
Applications: Bioassay
Applications: Bioassay
Recombinant Mouse FGF-basic (Ala11-Ser154, Accession #
NM_008006) was expressed in E. coli and purified to greater
than 98% purity.
432
Toll Free 1.877.BIOLEGEND (246.5343)
Bioactive Protein: This IFN-γ
protein is biologically active
Inhibition of WEHI-279
with an ED50 = 0.2 to 0.6 ng/
cell proliferation induced
mL, corresponding to a specific
by mouse IFN-γ
activity of 1.67 to 5 × 106 units/
mg, as determined by a inhibition of WEHI-279 cell proliferation
induced by mouse IFN-γ. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
ng/mL
Interleukin-1β (IL-1β) is synthesized as a 31 kD precursor that is
proteolytically cleaved by IL-1β converting enzyme to generate
the released ~17 kD bioactive IL-1β. IL-1β is a potent pro-inflammatory cytokine and induces many responses, including activation
of B and T cells. IL-1β binds to IL-1 receptor type 1 which contains
the intracellular signaling domain(s) and to the IL-1 receptor type
II which lacks the intracellular signaling domain. The IL-1 receptor type II is also shed from cells and binds and neutralizes IL-1β.
Recombinant Mouse IL-1β
Applications: Bioassay
Please request a quote for amounts greater than 500 µg.
Mouse IL-1α
Other Names: IL-1F1, LAF, EP, LEM, MCF/MNCF
Interleukin-1α (IL-1α) is synthesized as a 31 kD precursor that is
proteolytically cleaved by IL-1β converting enzyme to generate
the released ~17 kD bioactive IL-1α. IL-1α is a potent pro-inflammatory cytokine and induces many responses in many cell types
including lymphocytes, fibroblasts, and epithelial cells. IL-1α binds
to IL-1 receptor type 1 which contains the intracellular signaling
domain(s) and to the IL-1 receptor type II which lacks the intracellular signaling domain. The IL-1 receptor type II is also shed from
cells and binds and neutralizes IL-1α.
Recombinant Mouse IL-1α
Recombinant Mouse IL-1β (Val
118–Ser 269, Accession
#NM_008361) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
575302 575304 575306 575308
25 μg 100 μg 500 μg
10 μg
$195
$125
$300
$600
Bioactive Protein: This IL-1β
protein is biologically active
D10S cell proliferation
with an ED50 of 0.001 to 0.005
induced by mouse IL-1β
ng/mL, corresponding to a
specific activity of 0.2 to 1 × 109 units/mg, as determined by
the dose dependent stimulation of D10S cell proliferation.
Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200
µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and above.
pg/mL
Bioactive Protein (Carrier-Free)
575102
10 μg
$175
575104
25 μg
$295
575106
100 μg
$895
575108
500 μg
$2100
Please request a quote for amounts greater than 500 µg.
Applications: Bioassay
Recombinant Mouse IL-1α (Ser
115–Ser 270, Accession #
NM_010554) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
Other Names: H1, IFNβ-inducing factor, IL-β, OAF,
Catabolin
Bioassay-Validated Recombinant Cytokines
Bioactive Protein (Carrier-Free)
Mouse IL-1β
Bioactive Protein: This IL-1α
protein is biologically active
Proliferation of DS10 cells
with an ED50 of 1 to 5 pg/mL,
induced by mouse IL-1α
corresponding to a specific
activity of 0.2 to 1 × 109 units/mg, as determined by the dose
dependent stimulation of D10S cells proliferation. Endotoxin is
less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes
10–100 µg and at higher concentrations for sizes of 500 µg and
above.
pg/mL
Bioactive Protein (Carrier-Free)
575002 575004 575006 575008
25 μg 100 μg 500 μg
10 μg
$295
$175
$795
$2100
Please request a quote for amounts greater than 500 µg.
Mouse IL-2
Other Names: TCGF, EDF, KHF, MAF-C I, TDF
Interleukin-2 (IL-2) homodimer is secreted by T-cells and binds to
high affinity receptor IL-2Rα/β/γ (α = CD55, β = CD122, γ = CD132)
and lower affinity IL-2Rβ/γ and (IL-2Rα)2. IL-2 induces proliferation
and/or differentiation of activated helper T-cells, tumor infiltrating
lymphocytes, macrophages, natural killer cells, oligodendrocytes
and antibody producing B-cells. IL-2 influences expansion and
maintenance of CD25+FOXP3+CD4+ T-regulatory cells and thus
affects anti-tumor responses.
Recombinant Mouse IL-2
Applications: Bioassay
Recombinant Mouse IL-2 (Ala
21–Gln 169, Accession
#NM_008366) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density 490 nm
Optical Density (450nm)
Recombinant Mouse IFN-γ (His
23–Cys 155, Accession #
NM_008337) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-2
protein is biologically active
HT-2 cell proliferation
with an ED50 of 0.10 to 0.40 ng/
induced by mouse IL-2
mL, corresponding to a specific
­activity of 2.5 to 10 × 106 units/mg, as determined by dose-­
dependent stimulation of HT-2 cells. Endotoxin is less than Continues
ng/mL
biolegend.com • [email protected]
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Mouse IL-2 continued
0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
Bioactive Protein (Carrier-Free)
575402 575404 575406 575408
25 μg 100 μg 500 μg
10 μg
$225
$125
$500
$1400
Please request a quote for amounts greater than 500 µg.
Interleukin-3 (IL-3) is produced by activated T cells, mast cells, eosinophils, keratinocytes, and astrocytes. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and bone marrow-derived macrophages. IL-3 induces proliferation and differentiation
of hematopoietic stem cells and lineage committed progenitor
cells. IL-3 effects on target cells can be multi-faceted and are often
influenced by other cytokines or growth factors.
Recombinant Mouse IL-3
Applications: Bioassay, ELISA
Recombinant Mouse IL-3 (Ala
27–Cys 166, Accession
#NM_010556) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-3
protein is biologically active
ng/mL
Bioactivity of mouse IL-3
with an ED50 of 0.100 ng /mL,
was tested by a proliferation
corresponding to a specific
assay using M-NFS-60 cells.
activity of 1 × 107 units/mg, as
determined by the dose-dependent stimulation of a M-NFS-60
cell proliferation assay. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
Proliferation/control
Bioactive Protein (Carrier-Free)
574308
500 μg
$2000
Please request a quote for amounts greater than 500 µg.
Mouse IL-5
Other Names: EDF, Eo-CSF, BCGF-2, BCDF-m, IgA-EF,
TRF-1
Interleukin-5 (IL-5) is a homodimeric, disulphide-linked protein
produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5
is a 113 amino acid protein with a reported molecular weight of
35 to 37 kD for the homodimeric protein. Mouse and human IL-5
are approximately 70% identical. IL-5 has been shown to promote
the growth of immature hematopoietic BFU-E progenitors and
stimulates the activation and differentiation of eosinophils, and
promotes the generation of cytotoxic lymphocytes. Mouse IL-5
induces the differentiation and proliferation of pre-activated B
cells and stimulates the production and secretion of IgM and IgA
by B cells stimulated with bacterial endotoxin.
Recombinant Mouse IL-5
Applications: ELISA, Bioassay
Bioactive Protein: This IL-5 protein is bioactive and can be
used for in vitro assays.
Bioactive Protein
563303
5 μg
$210
Other Names: Interferon-β2, BSF-2, CDF, HSF, HPGF
Other Names: BSF-1, IaIF, HCGF, MCGF-2, MFT, TCGF-2
Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated
T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell
growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper
subset development.
Recombinant Mouse IL-4
Applications: Bioassay
Proliferation/control
574306
100 μg
$875
Mouse IL-6
575502 575504 575506 575508
25 μg 100 μg 500 μg
10 μg
$295
$175
$900
$2200
Mouse IL-4
434
574304
25 μg
$345
Mouse IL-4, amino acids
His23-Ser140 (Accession#
NM_021283), was expressed in
293E cells using human IL-2
signal peptide, and purified to
greater than 95% purity.
Bioactive Protein: This IL-4
protein is biologically active
Interleukin-6 (IL-6) is a potent lymphoid cell growth factor that
stimulates the growth and survivability of certain B cells and
T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B
cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and
keratinocytes.
Recombinant Mouse IL-6
Applications: Bioassay, ELISA
Recombinant Mouse IL-6 (Phe
25–Thr 211, Accession #
NM_031168) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
Bioassay-Validated Recombinant Cytokines
Other Names: BP/BPA, Eo-CSF, HCGF, MGF/MCGF,
Multi-CSF, PCSA, Thy1 inducing factor
ng/mL
Bioactive Protein (Carrier-Free)
574302
10 μg
$185
Mouse IL-3
CTLL-2 proliferation
induced by mouse IL-4
with an ED50 = 2 to 4 ng/mL, corresponding to a specific
activity of 2.5 to 5 × 105 units/mg, as determined by CTLL-2
cell proliferation induced by mouse IL-4 in a dose-dependent
manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled
carrier-free at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and higher.
pg/mL
7TD1 cell proliferation
induced by mouse IL-6.
Bioactive Protein: This IL-6
protein is biologically active
with an ED50 < 0.01 ng/mL,
corresponding to a specific
activity of > 1 × 108 units/mg,
as determined by the dose-
Toll Free 1.877.BIOLEGEND (246.5343)
Bioactive Protein (Carrier-Free)
575702
10 μg
$125
575704 575706 575708
25 μg 100 μg 500 μg
$225
$550
$1600
Please request a quote for amounts greater than 500 µg.
Mouse IL-7
Other Names: Lymphopoietin-1 (LP-1), Pre-B cell
growth factor, Thymocyte growth factor
In adult mice, IL-7/IL-7R signaling up-regulates expression of early
B cell factor (EBF), which in turn, regulates expression of B cell-specific genes required for the transition from lymphoid progenitor
to pro-B cells. IL-7 also plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces
anti-apoptotic factors Bcl2 and Bcl-xL and inhibits pro-apoptotic
factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition,
IL-7 controls T-cell size and metabolism through the activation of
PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that
are essential for both T-cell homeostasis and activation in vivo.
CD4 T cell lymphopenia increases the expression of circulating
IL-7, and TGF-β induces IL-7 downregulation.
Recombinant Mouse IL-7
Application: Bioassay
Proliferation/control
Mouse IL-7, amino acids Glu26
-Ile154 (Accession #
NM_008371.4) was expressed in
insect cells, and purified to
greater than 95% purity.
Bioactive protein: ED50 = 1.0 to
2.5 ng/mL, corresponding to a
ng/mL
PHA activated PBL proliferation
specific activity of 0.4 to 1 × 106
induced by mouse IL-7
units/mg, as determined by
PHA-activated PBL proliferation induced by IL-7 in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Bottled carrier-free at 200 µg/mL for sizes up to 500 µg and at
higher concentrations for greater amounts.
Other Names: CSIF, B-TCGF, TGIF
Interleukin-10 (IL-10) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine
production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity
response, and stimulation of the Th2 cell response, which results
in elevated antibody production.
Recombinant Mouse IL-10
Applications: Bioassay, ELISA
Recombinant Mouse IL-10 (Ser 19–Ser 178 (Cys167Tyr), Accession
#NM_010548) was expressed in E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-10 protein is biologically active with
an ED50 of 1 to 2 ng/mL, corresponding to a specific activity of
0.5 to 1 × 106 units/mg, as determined by the dose-dependent
stimulation of MC/9 cell proliferation. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
Bioactive Protein (Carrier-Free)
575802 575804 575806 575808
25 μg 100 μg 500 μg
10 μg
$295
$175
$595
$2100
Please request a quote for amounts greater than 500 µg.
Mouse IL-12 (p70)
Other Names: NKSF, TSF
Interleukin-12 [IL-12 (p70)] is a disulfide-linked heterodimer composed of unrelated p40 (glycosylated) and p35 subunits. IL-12 acts
as a growth factor for activated human T and NK cells, enhances
the lytic activity of human NK cells, and stimulates the production
of IFN-γ by resting human PBMC. IL-12R is formed by two chains,
IL-12Rβ1 and IL-12Rβ2. IL-12Rβ1 is associated with the Janus kinase
(Jak) Tyk2 and binds IL-12 p40; IL-12Rβ2 is associated with Jak2 and
binds either the heterodimer or the p35 chain. Signaling through
the IL-12 receptor complex induces phosphorylation, dimerization, and nuclear translocation of several signal transducer and
activator of transcription (STAT) family members (STAT1, 3, 4, 5),
but most of the biological responses to IL-12 have been attributed to STAT4.
Recombinant Mouse IL-12 (p70)
Applications: Bioassay
ng/mL
Mouse splenocytes IFN-γ
production induced
by mouse IL-12.
Bioactive Protein (Carrier-Free)
577802 577804
10 μg
25 μg
$185
$325
Please request a quote for amounts greater than 500 µg.
Bioassay-Validated Recombinant Cytokines
Interleukin-7 (IL-7) was initially described as a pre B-cell growth
factor expressed in bone marrow stromal cells. IL-7 is essential
for normal murine B cell development, and plays a key role in
regulating the homeostasis and function of T-cells. The binding
of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc), which leads to activation of receptorassociated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The
activated JAK proteins, in turn, phosphorylate specific residues
on the IL-7R, creating docking sites for signaling molecules such
as STAT5, and to a lesser extent, STAT1 and STAT3.
Mouse IL-10
OD (450–570nm)
dependent stimulation of 7TD1 cell proliferation. Endotoxin is
less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes
10–100 µg and at higher concentrations for sizes of 500 µg and
above.
Recombinant mouse IL-12 (p70)
heterodimer: P40 (Accession #
NM_008352: Met23-Ser335)
and P35 (Accession #
NM_008351: Arg23-Ala215).
This protein was expressed in
Sf9 cells as secreted protein,
and purified to greater than
95% purity.
Bioactive Protein: This IL-12
protein is biologically active with an ED50 = 0.10 to 0.20 ng/mL,
corresponding to a specific activity of 0.5 to 1.0 × 107
Continues
biolegend.com • [email protected]
435
Mouse IL-12 (p70) continued
units/mg, as determined by the dose-dependent stimulation of
IFN-γ production by mouse splenocytes. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for
sizes 10–100 µg and at higher concentrations for sizes of 500 µg
and higher.
Bioactive Protein (Carrier-Free)
577002 577004 577006 577008
25 μg 100 μg 500 μg
10 μg
$350
$195
$1200 $3500
Please request a quote for amounts greater than 500 µg.
Mouse IL-12/IL-23 p40 (homodimer)
Mouse IL-13
Other Names: ALRH, BHR1, P600
Interleukin-13 (IL-13) is an immunoregulatory cytokine produced
primarily by activated Th2 lymphocytes. IL-13 shares 30% amino
acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion.
Recombinant Mouse IL-13
Applications: Bioassay
Recombinant Mouse IL-13 (Ser
26–Phe 131, Accession
#NM_008355) was expressed in
E. coli and purified to greater
than 98% purity.
IL-12 and IL-23 share the p40 subunit, which heterodimerizes
with IL-12 p35 or IL-23 p19 subunits, respectively, to form IL-12 or
IL-23. IL-12 p40 exists as a monomer and as a homodimer (IL-12
p80). IL-12 induction is relevant in asthmatic airway inflammation and IL-12 expression can be induced by mouse parainfluenza
type I (Sendai) virus in airway epithelial cells. In that experimental model, IL-12 induction is followed by excessive expression of
IL-12 p40 that could be further enhanced in IL-12 p35-deficient
mice. Overexpression of IL-12 p80 causes macrophage accumulation and contributes to airway inflammation and consequent
morbidity during viral bronchitis. Amplified epithelial IL-12 p40
expression and augmented concentrations of BAL fluid IL-12 p40
(but not IL-12 p70) has been detected in asthmatic subjects. Also,
it has been demonstrated that p80, but not IL-12 or p40, induces
macrophage chemotaxis that is independent of IL-12 and mediated through the cytoplasmic tail of IL-12b1. Additional studies
with transgenic mice suggest that overexpression of IL-12 p80
prior to a viral infection increases the number of resident airway
macrophages, which primes the host for a protective response
against a lethal respiratory viral infection. In addition, it has been
suggested that p80 functions as a competitive antagonist of IL-12
p70. Mouse Con A-activated splenocytes display identical binding
affinities for p80 and IL-12, and in these cells p80 competitively inhibited IL-12 binding and IL-12-dependent proliferation. Furthermore, p80 is able to inhibit IL-12-dependent IFN-γ production in
freshly isolated splenocytes.
Recombinant Mouse IL-12 (p40 homodimer)
Applications: Bioassay
OD (450–570nm)
Recombinant Mouse IL-12 p40
homodimer, amino acids
Met23-Ser335 (Accession #
NM_008352), was expressed in
insect cells and purified to
greater than 98% purity.
ng/mL
Bioactive Protein: This IL-12
p40 protein is biologically
active with an ED50 = 1 to 4
ng/mL corresponding to a specific activity of 0.25 to 1.0 × 106
units/mg, as determined by the dose dependent inhibition of
IL-12-dependent IFNγ production. Bottled carrier-free at 100 µg/
mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Mouse IL-12 p40 homodimer
is able to inhibit IL-12dependent IFNγ production.
Bioactive Protein (Carrier-Free)
573102
436
10 μg
$175
Proliferation/control
Bioassay-Validated Recombinant Cytokines
Other Name: p40 homodimer (p80)
Bioactive Protein: This IL-13
protein is biologically active
ng/mL
with an ED50 of 1.5 to 3.5 ng/
TF-1 cells proliferation
mL, corresponding to a specific
induced by mouse IL-13.
activity of 2.85 to 6.6 × 105
units/mg, as determined by the dose-dependent stimulation of
TF-1 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
Bioactive Protein (Carrier-Free)
575902 575904 575906 575908
25 μg 100 μg 500 μg
10 μg
$350
$175
$1100 $2500
Please request a quote for amounts greater than 500 µg.
Mouse IL-15
Interleukin-15 (IL-15) was discovered in the supernatant of Simian
kidney epithelial cell line CV-1/EBNA, as a soluble factor capable
of supporting proliferation of the IL-2-dependent cell line, CTLL2. IL-15 is a regulatory cytokine, produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important
role in immune response and shares many functions with IL-2.
For example, it stimulates the proliferation of activated T cells, NK
cells, and B cells, and induces immunoglobulin synthesis by B cells
stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes
the development of dendritic cells and induces the production
of proinflammatory cytokines from macrophages. IL-15 acts as a
bridge between innate and adaptive immunity because of its diverse roles in the immune system.
IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with
IL-2. IL-15 is normally not secreted in soluble form but is instead
held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in
trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and
intermittent proliferation.
Recombinant Mouse IL-15
Applications: Bioassay
Toll Free 1.877.BIOLEGEND (246.5343)
Bioactive Protein: This IL-15
protein is biologically active
CTLL-2 cell proliferation
with an ED50 = 2.5 to 7.5 ng/
induced by mouse IL-15
mL, corresponding to a specific
activity of 1.3 to 4 × 105 units/mg, as determined by the
dose-dependent stimulation of CTLL-2 cell proliferation. Bottled
lyophilized, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
ng/mL
Bioactive Protein (Carrier-Free)
Mouse IL-17A
Other Names: IL-17, CTLA-8
Interleukin-17A (IL-17A) is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines.
IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including:
the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts. In addition, IL-17A enhances the
surface expression of ICAM-1 in fibroblasts, activation of NF-κb,
and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells
(Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Mouse IL-17A
shows 87%, 63% and 57% sequence identity to rat IL-17A, human
IL-17A, and a protein encoded by the ORF13 gene of herpesvirus
Saimiri (HVS), respectively. Both recombinant and natural IL-17A
have been shown to exist as a disulfide-linked homodimeric glycoprotein containing two ~16 kD monomers.
Recombinant Mouse IL-17A
Applications: ELISA, Bioassay
Optical Density (450nm)
Recombinant Mouse IL-17A
(Ala26-Ala158, Accession
#NM_010552) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-17A
protein is biologically active
with an ED50 of 0.5 to 2 ng/mL,
corresponding to a specific
activity 0.5–2 × 106 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of
IL-6. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at
200 µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and above.
ng/mL
Mouse IL-6 induced by
mouse IL-17A in fetal
mouse skin fibroblasts.
Bioactive Protein (Carrier-Free)
576002 576004 576006 576008
25 μg 100 μg 500 μg
10 μg
$225
$125
$500
$1500
Please request a quote for amounts greater than 500 µg.
Interleukin-17A/F (IL-17A/F) is part of the IL-17 cytokine family
which consists of six structurally related proteins (IL-17A, B, C, D,
E, and F). IL-17A is expressed primarily by Th17 cells, a subset of
CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% amino acid sequence homology. Like IL-17A, IL17F mRNA and protein have been detected in Th17 cells. IL-17F
and IL-17A exist as homodimers, adopting a cystine knot motif
formed through the interactions of four cystines, one of which is
responsible for the interchain bonding. IL-17F and IL-17A can form
both homodimeric and heterodimeric proteins when expressed
in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. Mouse Th17 cells secrete IL17A/F as well as the homodimers IL-17A and IL-17F. IL-17A/F induces
IL-6 and KC (CXCL1) production in mouse fibroblasts and macrophages. IL-17A/F signals through IL-17RA and IL-17RC.
Bioassay-Validated Recombinant Cytokines
566301 566304
2 μg
100 μg
$75
$895
Mouse IL-17A/F Heterodimer
Recombinant Mouse IL-17A/F Heterodimer
Applications: Bioassay
Optical Density (450nm)
Proliferation/control
Recombinant Mouse IL-15
(Asn49-Ser162, Accession
#NM_008357) was expressed in
E. coli and purified to greater
than 95% purity.
ng/mL
Recombinant Mouse IL-17A
(Ala26–Ala158, Accession #
NM_010552) and mouse IL-17F,
amino acids Arg 29–Ala 161
(Accession #NM_145856) were
expressed in E. coli and purified
to greater than 95% purity.
IL-6 induced by IL-17A/F in
fetal mouse fibroblast
Bioactive Protein: This
IL-17A/F protein is biologically
active with an ED50 = 15 to 50 ng/mL, corresponding to a
specific activity 2.0 to 6.7 × 104 units/mg, as determined by a
dose dependent stimulation of fetal mouse skin fibroblasts
production of IL-6. Bottled lyophilized with no additives, and
endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
580802 580804 580806 580808
25 μg 100 μg 500 μg
10 μg
$385
$195
$1000 $3000
Mouse IL-17F
Interleukin-17F (IL-17F) belongs to the IL-17 cytokine family which
includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the
strongest homology to IL-17A. They share 50% amino acid sequence homology. Recently, an IL-17–IL-17F heterodimer was
found to be expressed in Th17 cells, together with IL-17 and IL17F homodimers. IL-17 and IL-17F gene promoters share the same
pattern of chromatin remodeling in differentiated Th17 cells. T cell
populations in vitro and in vivo have shown different ratios of IL-17
and IL-17F expression. IL-17, IL-17F regulates proinflammatory
gene expression in vitro. Studies in vivo using Il17a(-/-), Il17f(-/-),
and Il17a(-/-)Il17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collageninduced arthritis, and arthritis in Il1rn(-/-) mice. In contrast, both
IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in
the induction of neutrophilia in the lungs and in the exacerbation
of Ag-induced pulmonary inflammation.
Continues
biolegend.com • [email protected]
437
Mouse IL-17F continued
Mouse IL-23
Recombinant Mouse IL-17F
Application: Bioassay
Bioactive protein: ED50 = 300
to 600 ng/mL, corresponding to
CXCL1 induced by mouse IL-17F
a specific activity of 0.16 to 0.33
in fetal mouse skin fibroblasts
× 104 units/mg, as determined
by dose-dependent stimulation of fetal mouse skin fibroblasts
production of CXCL1. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled carrier-free at 200 µg/mL up to 100 µg size and at
higher concentrations for greater amounts.
Bioassay-Validated Recombinant Cytokines
ng/mL
Bioactive Protein (Carrier-Free)
576106
100 μg
$550
Recombinant Mouse IL-23
Applications: Bioassay
576108
500 μg
$1550
Please request a quote for amounts greater than 500 µg.
Mouse IL-22
Other Names: IL-TIF
ng/mL
Interleukin-22 (IL-22) was originally named IL-10-related T cell-derived inducible factor (IL-TIF) and is a member of the IL-10 family
of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24
and IL-26. IL-22 is produced by activated NK and T cells, particularly Th1 and Th17 cells. Th17 cells express IL-17A, IL-17F, IL-21 and
IL-22, but they are differentially regulated. Along with IL-17A and
IL-17F, IL-22 regulates genes associated with innate immunity of
the skin. IL-22 inhibits IL-4 production by Th2 cells, activates the
transcription factors STAT-1 and STAT-3 in several hepatoma cell
lines, and induces acute phase reactants in the liver and pancreas.
Recombinant mouse IL-22 is a 33.4 kD non-disulfide-linked homodimeric, non-glycosylated protein containing two 146 amino
acid polypeptide chains.
Recombinant Mouse IL-22
Applications: Bioassay
Recombinant Mouse IL-22 (Leu
34–Val 179, Accession
#NM_016971) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (450nm)
Interleukin-23 (IL-23) is a member of the IL-6 family of cytokines
and consists of two subunits, p19 and p40. The p19-p40 heterodimer is stabilized by a disulfide bond. The subunit p40 is shared by
IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial
cells and polarized T cells; nevertheless, p40 is not expressed by
these cells. Therefore, the availability of functional IL-23 is limited
by the expression of p40 and not p19. IL-23 exerts its biological
activities through the interaction with a heterodimeric receptor
complex composed of IL-12Rβ1 and IL-23R. IL-23 activates Janus
kinase (JAK)/signal transducer and activator of transcription signaling molecules (STAT). JAK2 is constitutively associated with the
IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.
Optical Density (450nm)
Optical Density (450nm)
Mouse IL-17F, amino acids
Arg29-Ala161 (Accession #
NM_145856) was expressed in
E. Coli, and purified to greater
than 98% purity.
Bioactive Protein: This IL-22
protein is biologically active
Mouse IL-22 induces IL-10
with an ED50 of 0.095 to 0.265
in human Colo205 cells.
ng/mL, corresponding to a
specific a­ ctivity of 0.38 to 1.05 × 107 units/mg, as determined
by a dose dependent stimulation of human Colo205 cells in
production of IL-10. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
pg/mL
Bioactive Protein (Carrier-Free)
Induction of mouse IL-17A in
splenocytes by mouse IL-23
Recombinant mouse IL-23
consists of two subunits linked
via a disulphide bond: P19
(Accession# NM_031252:
Met1-Ala 196) and P40 (Accession# NM_008352 : Met Met1-Ser
335). Mouse IL-23 was expressed
in insect cells, and purified to
greater than 95% purity.
Bioactive Protein: This IL-23 protein is biologically active
with an ED50 = 0.5 to 0.8 ng/mL, corresponding to a specific
activity of 1.25 to 2.0 × 106 units/mg, as determined by mouse
splenocytes IL-17A secretion induced by mouse IL-23 in a dosedependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at
higher concentrations for sizes of 500 µg and higher.
Bioactive Protein (Carrier-Free)
589002 589004 589006 589008
25 μg 100 μg 500 μg
10 μg
$575
$275
$1800 $5400
Please request a quote for amounts greater than 500 µg.
Mouse IL-25 (IL-17E)
Interleukin-25 (IL-25), also known as IL-17E, is a member of the IL-17
family, which includes IL-17A, IL-17B, IL-17C, IL-17F and IL-17A/F. Primary sequence homology differs between family members, with
hIL-17 and hIL-17F having the highest homology (44%), while hIL17 and hIL-25 have the lowest (15%). IL-25 induces elevated gene
expression of IL-4, IL-5, and IL-13 in multiple tissues and results in
T helper 2 (TH2)-type immune responses (increased serum IgE
levels) and pathologic changes in the lungs and digestive tract
with eosinophilic infiltrates, increased mucus production, epithelial cell hyperplasia and overall amplified allergic inflammation.
IL-25 shares the receptor IL-17RB with IL-17B, although it binds with
much higher affinity than IL-17B.
Recombinant Mouse IL-25 (IL-17E)
Applications: Bioassay
576202 576204 576206 576208
25 μg 100 μg 500 μg
10 μg
$385
$195
$1200 $2950
Please request a quote for amounts greater than 500 µg.
438
Toll Free 1.877.BIOLEGEND (246.5343)
Mouse IL-25 binding to
recombinant IL17BR/Fc Chimera
detected by functional ELISA
Bioactive Protein: Mouse IL-25
binds to recombinant mouse
IL17BR/Fc Chimera in a functional ELISA. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for
sizes 10–100 µg and at higher concentrations for sizes of 500 µg
and higher.
Bioactive Protein (Carrier-Free)
Please request a quote for amounts greater than 500 µg.
Mouse IL-27
Other Names: Interleukin 27, IL-27p28 (IL-30), EBI3
Interleukin IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 P40-related protein) and P28 (a newly
discovered IL-12 P35-related protein). It is a member of the IL-6/
IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on
T cells and NK cells. It has been reported that IL-27 drives rapid
clonal expansion of naïve CD4+ T cells, promotes Th1 polarization,
and IFN-γ production in synergy with IL-12. The IL-27 induced Th1
differentiation was mediated by rapid and marked up-regulation
of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27
has an anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+ T cell and NK
cell activation and a potential therapeutic role for autoimmune
disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein
gp130 serves as a functional signal-transducing molecule for IL-27.
Recombinant Mouse IL-27
Optical Density (450nm)
Applications: Bioassay
ng/mL
Inhibition of IL-2 production
induced by mouse IL-27 in
mouse activated splenocytes.
Recombinant mouse IL-27
consists of human CD33 signal
peptide (Met1-Ala16), mouse
EBI3 (NM_015766, Tyr19Pro228), and mouse p28
(NM_145636, Phe29-Ser234)
joined via a GGGSGGGSGG​
GTGGGS linker, expressed in
293E cells. This protein was
purified to greater than 95%
purity.
Bioactive Protein: This IL-27 protein is biologically active with
an ED50 = 5 to 10 ng/mL, corresponding to a specific activity
of 1.0 to 2.0 × 105 units/mg, as determined by inhibition of
IL-2 production induced by mouse IL-27 in mouse splenocytes
activated with anti-CD3 and anti-CD28 antibodies. The protein
is bottled carrier-free, and endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg).
Please request a quote for amounts greater than 500 µg.
Mouse IL-33
Other Names: Il-1f11, Nuclear factor from high
endothelial venules, NF-HEV
Interleukin-33 (IL-33) belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. These cytokines are synthesized as biologically inactive precursor and are cleaved by the
enzyme caspase-1 to be secreted as the mature active form. IL-33
stimulates target cells by binding to the IL-1R/TLR superfamily
member ST2 and subsequently activates NF-κB and MAPK pathways via identical signaling events to those observed for IL-1b.
IL-33 is a nuclear factor (NF-HEV) that is abundantly expressed in
high endothelial venules (HEV) lymphoid tissues, and some epithelial cells. It associates with chromatin and exhibits transcriptional regulatory properties.
Bioassay-Validated Recombinant Cytokines
579802 579804 579806 579808
25 μg 100 μg 500 μg
10 μg
$300
$195
$600
$2000
Bioactive Protein (Carrier-Free)
577402 577404 577406 577408
25 μg 100 μg 500 μg
10 μg
$395
$245
$1200 $4000
Recombinant Mouse IL-33
Applications: Bioassay
Recombinant Mouse IL-33
(Ser109 - Ile266, Accession #
NM_133775) was expressed in
E. coli and purified to greater
than 98% purity.
IL-5 (pg/mL)
Optical Density (450nm)
mIL-25 (ng/mL)
Recombinant mouse IL-25,
amino acids Val17-Ala169
(Accession # NM_080729) was
expressed in E. coli, and
purified to greater than 98%
purity.
Bioactive Protein: This IL-33
protein is biologically active
IL-5 induction by mouse IL-33
with an ED50 of 2 to 5 ng/mL,
in splenocytes activated by
corresponding to a specific
anti-CD3 and anti-CD28. Data
kindly provided Dr. Foo Y. Liew.
activity of 2 to 5 × 105 units/mg,
as determined by induction of
IL-5 in activated splenocytes by IL-33. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
580502 580504 580506 580508
25 μg 100 μg 500 μg
10 μg
$325
$175
$800
$2200
Please request a quote for amounts greater than 500 µg.
Mouse IL-34
Other Name: C16orf77 homolog
Mouse IL-34 shares a sequence identity of 71% with human IL-34
on the amino acid level, and the IL-34 gene is syntenic in the
human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share
sequence homology with M-CSF; nevertheless, it binds to the
CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines
have different ability to induce the production of chemokines
(MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells.
The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF.
Continues
biolegend.com • [email protected]
439
Mouse IL-34 continued
As a result, different bioactivities and signal activation kinetics/
strength are produced for these cytokines.
Bioactive Protein (Carrier-Free)
Recombinant Mouse IL-34
577502
Proliferation/Control
Recombinant mouse IL-34
(Accession# NM_001135100.1:
Met1–Pro235 with a C-terminal
8His tag) was expressed CHO
cells, and purified to greater
than 98% purity.
Bioactive Protein: This IL-34
protein is biologically active
M-NSF-60 cell proliferation
with an ED50 = 20 to 30 ng/mL,
induced by mouse IL-34.
corresponding to a specific
activity of 3.3 to 5 × 104 units/mg, as determined by M-NFS-60
cell proliferation induction in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at
100 µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and higher.
Bioassay-Validated Recombinant Cytokines
ng/mL
Bioactive Protein (Carrier-Free)
577602 577604 577606 577608
25 μg 100 μg 500 μg
10 μg
$595
$275
$1500 $4200
Please request a quote for amounts greater than 500 µg.
10 μg
$265
Mouse MCP-1 (CCL2)
Other Names: MCAF, JE, SCYA2, HC-11, P6, SMC-CF
Monocyte chemotactic protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF), was identified
based on its ability to chemoattract monocytes. Subsequently,
MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical
to the product of the JE gene, a PDGF inducible gene. MCP-1 is a
member of the beta (C-C) chemokine subfamily known as CCL2.
Recombinant Mouse MCP-1 (CCL2)
Applications: Bioassay
Recombinant Mouse MCP-1
(Gln24-Arg96, Accession #
NM_011333) was expressed in E.
coli and purified to greater than
98% purity.
Chemotaxis Index
Applications: Bioassay
Bioactive Protein: This MCP-1
protein is biologically active
THP-1 cell chemotaxis
with an ED50 of 8 to 15 ng/mL,
induced by mouse MCP-1.
corresponding to a specific
activity of 0.6 to 1.25 × 105 units/mg, as determined by the dose
dependent chemoattraction of THP-1 cells. Endotoxin is less
than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL.
ng/mL
Mouse Isthmin1
Other Names: Isthmin-1, Ism1
Isthmin is a secreted protein, initially identified in the Xenopus
midbrain-hindbrain organizer (MHB) or isthmus organizer, where
it is highly expressed. The MHB is an important signaling center
in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin
is a 454 amino acid protein containing a Thrombospondin Type
1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at
the C-terminal. The TSR domains are highly conserved with 98%
identity between mouse and human, and 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are
also highly conserved, with 99% identity between mouse and
human; and 91% identity between mouse and Xenopus. Mouse
isthmin nucleotide sequence is similar to the human chromosome
20 open reading frame 82 (C20orf82). The Ism1 gene is conserved
in human, chimpanzee, dog, cow, chicken, and zebrafish. Recently
it has been suggested that isthmin has angiostatic activity in vitro
and in vivo. Isthmin inhibits EC capillary network formation mainly
by interfering with the early stages of in vitro angiogenesis on
matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in presence of VEGF. Over-expression of Isthmin
in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads
to abnormal intersegmental vessel (ISV) formation in the trunk.
Recombinant Mouse Isthmin1
Applications: Bioassay
Recombinant mouse Isthmin1 (Accession# NM_001126490, Met
1 - Tyr 454) was expressed in 293E cells. This protein was purified to greater than 95% purity.
440
The protein is bottled carrier-free at 200 µg/mL, and endotoxin
is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
576502 576504
10 μg
25 μg
$195
$350
Mouse SCF
Other Names: Kit Ligand, KITLG, Mast cell growth
factor, MGF, Steel factor, SLF
SCF (KITL) is a hematopoietic growth factor which can synergize
with a number of other cytokines to stimulate growth of hematopoietic progenitors in vitro and stimulates blood cell production
in vivo. SCF is encoded by Sl (‘steel’), a gene critical to the development of several distinct cell lineages during embryonic life. KITL
was identified as a soluble protein; nevertheless, the predicted
amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from
a transmembrane precursor. Two splice variants have been described for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of
KITL induces the dimerization of the KIT molecule, followed by a
change in the configuration of the intracellular domain and the
autophosphorylation of the receptor, opening several docking
sites for signal transduction molecules. Dysregulation of SCF–KI
signaling and gain-of-function KIT mutations contributes to the
genesis of many cancers, like acute myeloid leukemia, gastrointestinal stromal tumors, and mastocytosis.
Toll Free 1.877.BIOLEGEND (246.5343)
Applications: Bioassay
Proliferation/Control
Recombinant mouse SCF,
amino acids Lys26-Ala189
(Accession# M59915), was
expressed in E. coli, and
purified to greater than 98%
purity.
ng/mL
TF-1 cell proliferation
induced by mouse SCF
Bioactive Protein (Carrier-Free)
579702 579704 579706 579708
25 μg 100 μg 500 μg
10 μg
$325
$175
$725
$1475
Please request a quote for amounts greater than 500 µg.
Mouse TACI-Fc Chimera
Other Names: TNFRSF13B, CD267
Transmembrane activator and calcium-modulator and cytophilin
ligand interactor (TACI) is a TNFR family member molecule and
part of the so-called BAFF system. This includes two ligands: B
cell-activating factor of the TNF family (BAFF) and a proliferationinducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C),
TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17).
BAFF binds the three receptors, while APRIL binds TACI and BCMA.
TACI is constitutively expressed on naïve B cells and is upregulated
on activated B cells. TACI levels are severely reduced in newborn
mice B cells compared with those of adult mice, and newborn B
cells do not secrete Igs when they are stimulated with BAFF or
APRIL. This reduced TACI expression might be responsible for the
irresponsiveness of newborn B cells to T cell independent type II
antigens such as bacterial capsular polysaccharides.
Recombinant Mouse TACI-Fc Chimera
Applications: Bioassay
Optical Density (490nm)
Mouse TACI (Accession #
NM_021349: Met1-Thr 129)
- SRENLYFQG- Human IgG (Pro
100 –Lys300) was expressed
293E cells, and purified to
greater than 98% purity.
ng/mL
Bioactive Protein: This TACI-Fc
Chimera protein is biologically
active with an ED50 = 5 – 10
ng/mL, corresponding to a specific activity of 1.0 to 2.0 × 105
units/mg, as determined by inhibition of BAFF mediated B cell
proliferation by mouse TACI-Fc in a dose-dependent manner.
Bottled carrier-free at 200 µg/mL for sizes 10–500 µg, and
endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Inhibition of BAFF mediated
B cell proliferation by
mouse TACI-Fc.
Please request a quote for amounts greater than 500 µg.
Mouse TNF-α
Other Names: TNF, Cachectin, Necrosin, MCF, DIF,
TNFSF-2
Tumor necrosis factor-α (TNF-α) is secreted by macrophages,
monocytes, neutrophils, T cells (principally CD4+), and NK cells.
Many transformed cell lines also secrete TNF-α. Monomeric
mouse TNF-α is a 156 amino acid protein (N-glycosylated) with
a reported molecular weight of 17.5 kD. TNF-α forms multimeric
complexes, and stable trimers are most common in solution. A
26 kD membrane form of TNF-α has also been described. TNF-α
binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines
in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis
in neutrophils.
Bioassay-Validated Recombinant Cytokines
Bioactive Protein: This SCF
protein is biologically active
with an ED50 = 15 ng/mL, corresponding to a specific activity
of 6.7 × 104 units/mg, as determined by the dose dependent
stimulation of TF-1 cell proliferation. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and higher.
Bioactive Protein (Carrier-Free)
577702 577704 577706 577708
25 μg 100 μg 500 μg
10 μg
$225
$125
$625
$1600
Recombinant Mouse TNF-α
Applications: Bioassay
Recombinant Mouse TNF-α
(Leu 80–Leu 235, Accession
#NM_013693) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (490nm)
Recombinant Mouse SCF
Bioactive Protein: This TNF-α
protein is biologically active
with an ED50 of 0.010 to 0.020
ng/mL, corresponding to a specific activity of 5 to 10 × 107
units/mg, as determined by a dose dependent cytotoxic effect
in L929 cells treated with actinomycin D. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100
µg and at higher concentrations for sizes of 500 µg and above.
ng/mL
Cytotoxic effect of mouse
TNF-α in L929 cells
Bioactive Protein (Carrier-Free)
575202 575204 575206 575208
25 μg 100 μg 500 μg
10 μg
$225
$125
$550
$1475
Please request a quote for amounts greater than 500 µg.
Mouse TRANCE (RANKL)
Other Names: TNFSF11, OPGL, ODF, CD254
The RANKL gene encodes a type II membrane protein of 316
amino acids with a predicted molecular mass of 35 kD. RANKL
is cleaved to produce a soluble form with biological activity. The
shedding of membrane-bound RANKL appears to be mediated
by expression of matrix metalloproteinase (MMP) 14 and ADAM10.
Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore, RANKL shedding seems to be
an important process that down-regulates local osteoclastogenesis. Alternatively, an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation, activation, and survival,
which results in increased bone resorption. Binding of RANKL to
its receptor RANK activates TNF receptor- associated factor Continues
biolegend.com • [email protected]
441
Mouse TRANCE continued
Recombinant Mouse TRANCE (RANKL)
Applications: Bioassay
Bioactive Protein: This TRANCE protein’s bioactivity was
measured by its property to induce osteoclast differentiation
in RAW264.7 cells in the absence of any cross-linking. The
bioactivity is equivalent to competitors’ cytokines. The protein
is bottled carrier-free at 200 µg/mL, and endotoxin is less than
0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
10 μg
Other Names: TCGF, EDF, KHF, MAF-C I, TDF
Interleukin-2 (IL-2) is a potent lymphoid cell growth factor which
exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to
stimulate growth and differentiation of B cells, NK cells, LAK cells,
monocytes, and oligodendrocytes.
Recombinant Rat IL-2
Applications: Bioassay
Recombinant Rat IL-2 (Ala21Met153, Accession #
NM_053836) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-2
protein is biologically active with
CTLL-2 proliferation
an ED50 of 0.102 to 0.295 ng/
induced by rat IL-2.
mL, corresponding to a specific
­activity of 3.39 to 9.8 × 106 units/mg, as determined by the dose
dependent stimulation of CTLL-2 cells. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL PBS for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and higher.
ng/mL
$200
Mouse VEGF120 Other Names: Vegf, Vegf-a, Vpf
VegfA belong to the VEGF family which has at least 7 members,
VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venomderived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A
has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA,
and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial
macrophages and synovial fibroblasts in the synovial tissues of
RA patients.
Recombinant Mouse VEGF120
Applications: Bioassay
Recombinant Mouse VEGF120,
(Ala27-Arg146, Accession #
S38100) was expressed in E. coli
and purified to greater than
98% purity
Bioactive Protein: This
VEGF120 protein is biologically
active with an ED50 of 1 to 4
ng/mL, corresponding to a specific activity of 0.25 to 1 × 106
units/mg, as determined by the dose dependent stimulation
of HUVEC cells proliferation. Endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at
higher concentrations for sizes of 500 µg and above.
ng/mL
VegfA induces the
proliferation of HUVEC cells
Bioactive Protein (Carrier-Free)
580902 580904 580906 580908
25 μg 100 μg 500 μg
10 μg
$325
$195
$800
$1800
Bioactive Protein (Carrier-Free)
579502 579504 579506 579508
25 μg 100 μg 500 μg
10 μg
$225
$125
$600
$1200
Please request a quote for amounts greater than 500 µg.
Rat IL-6
Other Names: Interferon-β2, BSF-2, CDF, HSF, HPGF
IL-6 is a multifunctional cytokine with both differentiation and
growth-promoting effects for a variety of target cell types. It is
a B-cell stimulatory factor inducing terminal differentiation and
high level antibody production in B lymphocytes, and it acts as
a hybridoma/plasmacytoma growth factor. IL-6 stimulates the
growth of hematopoietic stem cells and induces the synthesis
of acute phase plasma proteins. In addition, IL-6 and TNF-α are
involved in osteoclastogenesis and are responsible for bone resorption; both cytokines participate in the pathogenesis of osteoporosis. Also, IL-6 is systemically elevated in obesity and is a
predictive factor in type 2 diabetes. Furthermore, IL-6 regulates
pancreatic alpha cell mass expansion and plays a pivotal role in
liver regeneration. Rat IL-6 has 92% and 68% identity to the mouse
and human nucleotide sequence, respectively.
Recombinant Rat IL-6
Applications: Bioassay
Recombinant Rat IL-6 (Phe25Thr211, Accession #
NM_012589) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
577102
Proliferation/control
Bioassay-Validated Recombinant Cytokines
Recombinant mouse TRANCE, amino acids Lis158-Asp316 (Accession # NM_011613.3) was expressed in insect cells, with an Nterminal 9-His tag and IEGR-Xa sequence. This protein was purified to greater than 95% purity.
Rat IL-2
Proliferation/control
6 (TRAF6), which is linked to downstream pathways including NFkB, c-jun N-terminal kinase (JNK) or Src. TRAF6 has been shown
to be necessary for the differentiation of osteoclastic cells by enhancing Src kinase, essential for osteoclast function.
Please request a quote for amounts greater than 500 µg.
pg/mL
7TD1 cell proliferation
induced by rat IL-6.
442
Bioactive Protein: This IL-6
protein is biologically active
with an ED50 < 0.01 ng/mL,
corresponding to a specific
activity of > 1 × 108 units/mg,
Toll Free 1.877.BIOLEGEND (246.5343)
Bioactive Protein (Carrier-Free)
579902 579904 579906 579908
25 μg 100 μg 500 μg
10 μg
$225
$125
$550
$1600
Please request a quote for amounts greater than 500 µg.
Recombinant Human CXCL9 (MIG)
Application: Bioassay
Human CXCL9, amino acids
Thr23-Thr125 (Accession#
NM_002416.1), was expressed
in E. coli, and purified to greater
than 98% purity.
Chemotaxis Index
as determined by the dose dependent stimulation of 7TD1
cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and higher.
Bioactive Protein: Human
CXCL9 is biologically active
and chemoattracts human
T cells activated with PHA and IL-2 in a dose-dependent
manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled
carrier-free at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and higher.
ng/mL
Human T cells chemoattracted
by human CXCL9
Other Names: TNFSF2, TNF, MCF Cachectin,
Necrosin, Macro­phage cytotoxic factor, DIF
Recombinant Rat TNF-α
Applications: Bioassay, ELISA
Optical Density (450nm)
Recombinant Rat TNF-α
(Leu80-Leu235, Accession #
NM_012675) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This TNF-α
protein is biologically active
with an ED50 of 5 to 15 pg/mL,
corresponding to a specific activity of 0.66 to 2 × 108 units/mg,
as determined by a dose dependent cytotoxicity assay using
L929 cells treated with actinomycin D. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
ng/mL
Rat TNF-α cytotoxicity
on L929 cells.
Bioactive Protein (Carrier-Free)
580102 580104 580106 580108
25 μg 100 μg 500 μg
10 μg
$295
$150
$750
$1800
Please request a quote for amounts greater than 500 µg.
Human CXCL9 (MIG)
Other Name: SCYB9
CXCL9 is a member of the α subfamily of chemokines that lacks
the ELR domain. CXCL9 has been shown to be a chemoattractant for activated T lymphocytes and TIL, but not for neutrophils
or monocytes. A chemokine receptor (CXCR3) specific for CXCL9
and IP10 has been cloned and shown to be highly expressed in
IL-2 activated T lymphocytes.
Bioassay-Validated Recombinant Cytokines
TNF-α is secreted by macrophages, monocytes, neutrophils,
T cells (principally CD4+), and NK cells following their stimulation
by bacterial lipopolysaccharide and cytokines (IL-2, GM-CSF, interferons). Many transformed cell lines also secrete TNF-α. Monomeric rat TNF-α is a 158 amino acid protein (N-glycosylated)
with a reported molecular weight of 17.3 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A
26 kD membrane form of TNF-α has also been described. TNF-α
binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines
in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis
in neutrophils.
Bioactive Protein (Carrier-Free)
578102
10 μg
$125
578104
25 μg
$225
578106
100 μg
$595
578108
500 μg
$2400
Please request a quote for amounts greater than 500 µg.
Human FGF-basic/145 aa
Other Names: bFGF, FGF-2, Heparin-binding growth
factor
Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding
growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in
vivo. FGF-basic has been isolated from neural, pituitary, adrenal
cortex, and placental tissues.
Recombinant Human FGF-basic/145 aa
Applications: Bioassay
Recombinant Human FGF-basic
(Ala 144–Ser 288, Accession #
NM_002006) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (490 nm)
Rat TNF-α
Bioactive Protein: This
FGF-basic protein is biologi3NIH/3T3 cell proliferation
cally active with an ED50 of 1
induced by human FGFb
to 4 ng/mL, corresponding to
a specific activity of 0.25 to 1 × 106 units/mg, as determined by
the dose dependent stimulation of NIH/ 3T3 cell proliferation.
Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200
µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
571502
10 μg
$125
571504
25 μg
$225
571506
100 μg
$550
571508
500 μg
$1600
Please request a quote for amounts greater than 500 µg.
Human G-CSF
Other Names: CSF-β, G-CSA, MGI-2, Pluripoietin
Granulocyte-colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophilic
Continues
biolegend.com • [email protected]
443
Human G-CSF continued
granulocyte lineage. In addition, G-CSF can enhance the survival
and activate the immunological functions of mature neutrophils.
G-CSF is produced primarily by monocytes and macrophages
upon activation by endotoxin, TNF-α, or IFN-γ.
Recombinant Human G-CSF
Applications: ELISA, Bioassay
Human G-CSF is an 18.7 kD protein containing 174 amino acids.
Bioactive Protein: This G-CSF protein is bioactive and can be
used for in vitro assays.
Bioactive Protein: This IFN-γ protein is biologically active with
an ED50 of 0.2–1 ng/mL, corresponding to a specific activity of 1
to 5 × 106 units/mg, as determined by dose-dependent stimulation of HT-29 cells. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled at 100 µg/mL PBS for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
570202 570204 570206 570208
25 μg 100 μg 500 μg
10 μg
$125
$70
$195
$600
$125
Human GM-CSF
Please request a quote for amounts greater than 500 µg.
Other Names: CSF-α, Eo-CSF, BPA, Pluripoietin-α
Human IL-1α
Granulocyte/macrophage-colony stimulating factor (GM-CSF) is
a hematopoietic factor that is produced by activated T-cells, Bcells, mast cells, macrophages, fibroblasts, and endothelial cells.
In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid,
megakaryocyte and eosinophil progenitors.
Other Names: LAF, EP, LEM, MCF/MNCF
Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are
the products of distinct genes, but are recognized by the same
cell surface receptors. IL-1α is a potent immuno-modulator which
mediates a wide range of immune and inflammatory responses.
Recombinant Human IL-1α
Recombinant Human GM-CSF
Applications: Bioassay, ELISA
Recombinant Human GM-CSF
(Ala18–Glu144, Accession #
NM_000758) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This
GM-CSF protein is biologically
TF-1 cell proliferation induced
active with an ED50 of 0.10 to
by human GM-CSF
0.30 ng/mL, corresponding to
a specific activity of 0.33 to 1 × 107 units/mg, as determined by
dose-­dependent TF-1 cell proliferation. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100
µg and at higher concentrations for sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
572902 572903 572904 572905
25 μg 100 μg 500 μg
10 μg
$225
$125
$695
$1600
Please request a quote for amounts greater than 500 µg.
Recombinant Human IL-1α (Ser
113–Ala 271, Accession #
NM_000575) was expressed in
E. coli and purified to greater
than 98% purity. Recombinant
human IL-1α is an 18.0 kD
protein containing 159 amino
acids.
Proliferation/control
Applications: Bioassay
Proliferation/control
Bioassay-Validated Recombinant Cytokines
2 μg
Recombinant Human IFN-γ (Gln 24–Gln 166, Accession #
NM_000619) was expressed in E. coli and purified to greater
than 98% purity.
Bioactive Protein (Carrier-Free)
Bioactive Protein (Carrier-Free)
561701
Applications: Bioassay
Bioactive Protein: This IL-1α
protein is biologically active
with an ED50 of 0.58 to 1.45 pg/
mL, corresponding to a specific activity of 0.689 to 1.72 × 109
units/mg, as determined by the dose-dependent stimulation of
D10 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
pg/mL
D10S cell proliferation
induced by human IL-1α
Bioactive Protein (Carrier-Free)
570002 570004 570006 570008
25 μg 100 μg 500 μg
10 μg
$395
$195
$900
$2100
Human IFN-γ
Please request a quote for amounts greater than 500 µg.
Other Names: Immune interferon, Type II
interferon, T-cell interferon, MAF
Human IL-1β
Interferon-γ (IFN-γ) is a potent multifunctional cytokine which
is secreted primarily by activated NK-cells and T-cells. Originally
characterized based on anti-viral activities, IFN-γ also exerts antiproliferative, immunoregulatory, and proinflammatory activities.
IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Recombinant Human IFN-γ
Other Names: H1, OAF, Catabolin, IFNβ-inducing
factor
Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are
the products of distinct genes, but are recognized by the same
cell surface receptors. IL-1β is a potent immuno-modulator which
mediates a wide range of immune and inflammatory responses,
including the activation of B and T-cells.
Recombinant human IFN-γ is a 16.7 kD protein containing 143
amino acids.
444
Toll Free 1.877.BIOLEGEND (246.5343)
Applications: Bioassay, ELISA
Recombinant Human IL-1β (Ala117-Ser269, Accession #
NM_000576) was expressed in E. coli and purified to greater
than 98% purity. Recombinant human IL-1β is a 17.3 kD protein
containing 153 amino acids.
Bioactive Protein: This IL-1β protein is biologically active with
an ED50 of 0.858 to 1.85 pg/mL, corresponding to a specific
activity of 0.54 to 1.16 × 109 units/mg, as determined by the
dose dependent stimulation of D10 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL
for sizes 10–100 µg and at higher concentrations for sizes of 500
µg and above.
Please request a quote for amounts greater than 500 µg.
Human IL-2
Other Names: TCGF, EDF, KHF, MAF-C I, TDF
Interleukin-2 (IL-2) was discovered through its function as a T cell
growth factor (TCGF), and plays a pivotal role in immune responses against pathogenic infection. Recognition and binding
of the foreign Ags by the TCRs stimulate both the secretion of IL-2
and the expression of IL-2Rs on the T cell surface. Subsequently,
the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK,
JAK/STAT, and PI3K/AKT signal pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected
cytotoxic T cells. Human IL-2 acts on murine and human T cells.
IL-2Rα is an IL-2-­specific receptor, IL-2Rβ is shared with IL-15, and
the γc is a common receptor shared by many cytokines including
IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Recombinant Human IL-2
Applications: Bioassay
Proliferation/control
Human IL-2, amino acids
Ala21-Thr153 (Accession #
NM_000586), was expressed in
insect cells, and purified to
greater than 95% purity.
Bioactive Protein: This IL-2
protein is biologically active
with an ED50 = 0.175 to 0.8
ng/mL, corresponding to a specific activity of 1.25 to 5.71 × 106
units/mg, as determined by the dose-dependent stimulation
of CTLL2 cell proliferation. Bottled carrier-free at 200 µg/mL
for sizes 10–100 µg, and endotoxin is less than 0.1 EU/µg (<0.01
ng/µg).
ng/mL
CTLL-2 cell proliferation
induced by human IL-2
Bioactive Protein (Carrier-Free)
589102 589104 589106
10 μg
25 μg 100 μg
$125
$225
$595
Please request a quote for amounts greater than 100 µg.
Other Names: BP/BPA, Eo-CSF, HCGF, MGF/MCGF,
Multi-CSF, PCSA, Thy1 inducing factor
Interleukin-3 (IL-3) is a hematopoietic growth factor involved in
the survival, proliferation, and differentiation of multipotent hematopoietic cells. IL-3 is the most potent growth factor for basophils, followed by GM-CSF and IL-5. These cytokines also act on
mature basophils through specific receptors, thereby mediating
adhesion, migration, and releasability. IL-3 is highly expressed by
mast cells, and large and rapidly released amounts of autocrine
IL-3 production is responsible for mast cell survival by IgE in the
absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma,
atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes,
neutrophils, and macrophages from bone marrow cultures. IL-3
plays a vital role in stimulating basophils and mast cell responses
to parasite infections, and since basophils play an important role
in Th2 immune responses, IL-3 is a critical regulator of allergic inflammation. IL-3 is expressed in the major embryonic vessels and
regulates the survival and proliferation of hematopoietic stem
cells in the early stages of embryonic development.
Bioassay-Validated Recombinant Cytokines
Bioactive Protein (Carrier-Free)
579402 579404 579406 579408
25 μg 100 μg 500 μg
10 μg
$295
$175
$1100
$2100
Human IL-3
Recombinant Human IL-3
Application: Bioassay
Human IL-3, amino acids
His23-Ser140 (Accession#
NM_021283), was expressed in
sf9 cells with C-terminal His-8,
and purified to greater than
95% purity.
Proliferation/control
Recombinant Human IL-1β
Bioactive Protein: ED50 = 0.05
– 0.3 ng/mL, corresponding to a
specific activity of 0.33 – 2 × 107
units/mg, as determined by the dose-dependent stimulation
of TF-1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg.
ng/mL
TF-1 cell proliferation
induced by human IL-3
Bioactive Protein (Carrier-Free)
578002 578004 578006 578008
25 μg 100 μg 500 μg
10 μg
$295
$150
$495
$1175
Please request a quote for amounts greater than 500 µg.
Human IL-4
Other Names: BCGF-1, BSF-1, LSF-1
Interleukin-4 (IL-4) is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with
allergy and asthma. The Type I receptor comprises IL-4Rα and the
common gamma-chain (γc), which is also shared by the cytokines
IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can
use the type II complex, comprising IL-4Rα and IL-13Rα1, which is
present in non-hematopoietic cells. This second receptor complex
is a functional receptor for IL-13, which shares approximately 25%
homology with IL-4. The type I receptor complex can be formed
only by IL-4 and is active in Th2 development. In contrast, the
type II receptor complex formed by either IL-4 or IL-13 is most active during airway hypersensitivity and mucus secretion and is
not found in T cells.
Continues
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445
Human IL-4 continued
Applications: Bioassay
Bioactive Protein: This IL-4
protein is biologically active
with an ED50 = 0.2 to 0.6 ng/
mL, corresponding to a specific activity of 1.65 to 5 × 106 units/
mg, as determined by the dose dependent stimulation of TF-1
cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at
higher concentrations for sizes of 500 µg and higher.
ng/mL
Bioassay-Validated Recombinant Cytokines
TF-1 cell proliferation
induced by human IL-4
Bioactive Protein (Carrier-Free)
Human IL-7
Please request a quote for amounts greater than 500 µg.
Human IL-5
Other Names: EDF, Eo-CSF, BCGF-2, BCDF-m, IgA-EF,
TRF-1
Interleukin-5 (IL-5) is a homodimeric, disulphide-linked protein
produced by T-cells. Monomeric human IL-5 is a 126 amino acid
protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70%
identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation
and differentiation of eosinophils, and promote the generation
of cytotoxic lymphocytes.
Recombinant Human IL-5
Applications: ELISA, Bioassay
Bioactive Protein: This IL-5 protein is bioactive and can be
used for in vitro assays.
Bioactive Protein
2 μg
Bioactive Protein (Carrier-Free)
570802 570804 570806 570808
25 μg 100 μg 500 μg
10 μg
$225
$125
$650
$1500
Please request a quote for amounts greater than 500 µg.
574002 574004 574006 574008
25 μg 100 μg 500 μg
10 μg
$225
$125
$675
$1800
560701
Bioactive Protein: This IL-6
protein is biologically active
7TD1 cell proliferation
with an ED50 of 4 to 6 pg/mL,
induced by human IL-6
corresponding to a specific
activity of 1.6 to 2.5 × 108 units/mg, as determined by a dose
dependent stimulation in a 7TD1 cell proliferation assay.
Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200
µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and above.
ng/mL
$125
Other Names: LP-1, Pre-BCGF, TGF
Interleukin-7 (IL-7) is a potent lymphoid cell growth factor which
promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells
and CTLs, and can stimulate tumoricidal activity of monocytes
and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells.
Recombinant Human IL-7
Applications: Bioassay
Recombinant Human IL-7
(Asp26-His177, Accession #
NM_000880) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (490 nm)
Proliferation/control
Recombinant Human IL-4,
amino acids His25-Ser153
(Accession # NM_000589), was
expressed in E. Coli, and
purified to greater than 95%
purity.
Recombinant Human IL-6 (Pro
29–Met 212, Accession #
NM_000600) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
Recombinant Human IL-4
Bioactive Protein: This IL-7
protein is biologically active
Mouse IXN/2B cell proliferation
with an ED50 <0.5 ng/mL,
induced by human IL-7
corresponding to a specific
activity of greater than 2.0 × 106 units/mg, as determined by the
dose dependent stimulation of mouse IxN/2b cell proliferation.
ng/mL
Bioactive Protein (Carrier-Free)
Human IL-6
566501 566503
2 μg
100 μg
$95
$1100
Other Names: BSF-2, CDF, HSF, HPGF
Interleukin-6 (IL-6) is a potent lymphoid cell growth factor that
stimulates the growth and survival of certain B-cells and T-cells.
IL-6 plays a role in host defense, acute phase reactions, immune
response, and hematopoiesis. IL-6 is expressed by T-cells, B-cells,
monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
Recombinant Human IL-6
Recombinant human IL-6 is a 26 kD protein containing 184
amino acids.
Applications: Bioassay, ELISA
446
Human IL-8
Other Names: SCYB8, MDNCF, NAP-1, GCP1, CXCL8
Interleukin-8 (IL-8), also known as neutrophil chemotactic factor,
neutrophil activating protein, and monocyte-derived neutrophil
chemotactic factor, is a member of the alpha (C-X-C) subfamily of
chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and
degranulation. The 72 amino acid IL-8 is the predominant form
secreted by monocytes and lymphocytes.
Toll Free 1.877.BIOLEGEND (246.5343)
Recombinant Human IL-8
Recombinant Human IL-10 (Mammalianexpressed)
ng/mL
Neutrophils Chemoattracted
by Human IL-8 bioactivity
Recombinant human IL-8,
amino acids Ser28-Ser99
(Accession # NM_000584) was
expressed in E. coli, and
purified to greater than 95%
purity.
Bioactive Protein (Carrier-Free)
574202
10 μg
$125
574204
25 μg
$225
574206
100 μg
$580
574208
500 μg
$1400
ng/mL
Human IL-10 inhibits the
production of IFNγ in
PMA activated PBMC
Bioactive Protein: This IL-10
protein is biologically active
with an ED50 = 0.1 to 0.3 ng/mL corresponding to a specific
activity of 3.3 to 10 × 106 units/mg, as determined by the dose
dependent inhibition of IFN-γ production by PMA activated
PBMC. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled
carrier-free at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and higher.
Bioactive Protein (Carrier-Free)
Please request a quote for amounts greater than 500 µg.
Human IL-10
573202 573204 573206
10 μg
25 μg 100 μg
$195
$395
$1200
Please request a quote for amounts greater than 100 µg.
Other Name: CSIF
Interleukin-10 (IL-10) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence
homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated
cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which
results in elevated antibody production.
Recombinant Human IL-10
(E. Coli-expressed)
Recombinant human IL-10 is an 18.6 kD protein containing 160
amino acids.
Human IL-12 (p70)
Other Names: NKSF, CLMF
Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12
is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of
disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked
p40 homodimer can bind to IL-12 receptors and antagonize the
activities of bioactive IL-12.
Recombinant Human IL-12 (p70)
Applications: Bioassay
Recombinant Human IL-10 (Ser
19–Asn 178, Accession #
NM_000572) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-10
protein is biologically active
with an ED50 of 0.8 to 1.5 ng/
mL corresponding to a specific activity of 0.66 to 1.25 × 106
units/mg, as determined by the dose dependent stimulation of
MC/9 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and higher.
ng/mL
MC/9 cell proliferation
induced by human IL-10
Bioactive Protein (Carrier-Free)
571002 571004 571006 571008
25 μg 100 μg 500 μg
10 μg
$350
$175
$1100 $2500
Please request a quote for amounts greater than 500 µg.
Optical Density (450nm)
Applications: Bioassay
Proliferation/control
Recombinant Human IL-10,
amino acids Ser19-Asn178
(Accession # NM_000572), was
expressed in 293E cells, using
human IL-2 signal peptide, and
purified to greater than 95%
purity.
ng/mL
IFNγ induced by human IL-12
in PBMC activated with PHA
Bioassay-Validated Recombinant Cytokines
Bioactive Protein: This IL-8
protein’s bioactivity was
measured by its property to chemoattract human peripheral
blood neutrophils in a dose-dependent manner. The highest
chemotactic activity was detected from 10 to 100 ng/mL. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at
200 µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and higher.
Applications: Bioassay
Optical Density (450nm)
Chemotaxis Index
Applications: Bioassay
Recombinant Human IL-12 (p70)
consists of two subunits linked
via a disulphide bond: P35
(Accession# NM_000882: Arg
57- Ser 253) and P40 (Accession# NM_002187: Ile 23-Ser
328). This IL-12 protein is
expressed in insect cells as
secreted protein and purified to
greater than 95% purity.
Bioactive Protein: This IL-12
protein is biologically active with an ED50 = 0.05 to 0.10 ng/mL,
corresponding to a specific activity of 1.0 – 2.0 × 107 units/mg,
as determined by the production of IFNγ by activated human
PBMC in response to IL-12. Endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg). Bottled carrier-free at 100 µg/mL for sizes 10–100
µg and at higher concentrations for sizes of 500 µg and higher.
Bioactive Protein (Carrier-Free)
573002 573004 573006 573008
25 μg 100 μg 500 μg
10 μg
$395
$195
$1200 $2600
Please request a quote for amounts greater than 500 µg.
biolegend.com • [email protected]
447
Human IL-13
Other Names: NC30, ALRH, BHR1, P600
Interleukin-13 (IL-13) is an immunoregulatory cytokine produced
primarily by activated Th2 lymphocytes. IL-13 shares 30% amino
acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion.
Recombinant Human IL-13
Applications: Bioassay, ELISA
Recombinant Human IL-13 (Gly
21–Asn 132, Accession #
NM_002188) was expressed in
E. coli and purified to greater
than 98% purity.
Proliferation/control
Bioactive Protein: This IL-13
protein is biologically active
ng/mL
with an ED50 of 1.5 to 3 ng/mL,
TF-1 cells proliferation
corresponding to a specific
induced by human IL-13
activity of 3.3 to 6.6 × 105 units/
mg, as determined by the dose dependent stimulation of TF-1
cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
Bioactive Protein (Carrier-Free)
571102
10 μg
$195
571104
25 μg
$395
571106
100 μg
$1200
Please request a quote for amounts greater than 500 µg.
Human IL-17A
Other Names: IL-17, CTLA-8
Interleukin-17A (IL-17A) is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines.
IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including:
the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial cells, endothelial cells and fibroblasts. In addition, IL-17A induces expression of ICAM in fibroblasts, activation of NF-κB, and
costimulation of T-cell proliferation. Studies conducted in mice
demonstrated that activated IL-17-secreting CD4+ helper T-cells
(Th17 cells) mediate an autoimmune arthritis that clinically and
immunologically resembles rheumatoid arthritis (RA). Human IL17A is closely related to a protein encoded by the ORF13 gene
of herpesvirus saimiri (HVS13) with overall sequence identity of
72% at the amino acid level and 75% at the nucleotide level. The
sequence homology between human IL-17A and mouse IL-17A
is 63% at the amino acid level and 72% at the nucleotide level.
Both recombinant and natural IL-17A have been shown to exist
as a disulfide-linked homodimeric glycoprotein containing two
~16 kD monomers.
Please request a quote for amounts greater than 500 µg.
Other Name: MGC9721
Interleukin-15 (IL-15) is an immunomodulating cytokine that is
secreted mainly by mononuclear phagocytes following infection
by viruses. Human IL-15 shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. IL-15
shares many biological properties with IL-2 including stimulatory
activities on the proliferation, survival, and activation of T lymphocytes and NK-cells. Recent reports have also suggested that that
IL-15 plays important roles in the pathogenesis of rheumatoid arthritis via activation of T-cells and induction of IL-17 production.
Recombinant human IL-15 is a ~13 kD single non-glycosylated
polypeptide chain.
Proliferation/control
Recombinant Human IL-15 (Asn
49–Ser 162, Accession #
NM_000585) was expressed in
E. coli and purified to greater
than 98% purity.
ng/mL
Recombinant Human IL-17A (Ile
20–Ala 155, Accession #
NM_002190) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein: This IL-17A
protein is biologically active
Induction of IL-6 in human
with an ED50 of 2 to 4 ng/mL,
dermal fibroblast by IL-17A
corresponding to a specific
activity 2.5 to 5 × 105 units/mg, as determined by a dose
dependent stimulation of normal human dermal fibroblasts
production of IL-6. Endotoxin is less than 0.1 EU/µg (<0.01 ng/
µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
Applications: Bioassay, ELISA
CTLL-2 cell proliferation
induced by human IL-15
Recombinant Human IL-17A
Applications: Bioassay, ELISA
Human IL-15
Recombinant Human IL-15
448
Bioactive Protein (Carrier-Free)
570302 570304 570306 570308
25 μg 100 μg 500 μg
10 μg
$395
$195
$1100
$2100
Optical Density (450 nm)
Bioassay-Validated Recombinant Cytokines
Recombinant human IL-13 is a 12.5 kD protein containing 114
amino acids.
ng/mL, corresponding to a specific activity of 5.88 to 8.33 × 106
units/mg, as determined by the dose dependent stimulation of
CTLL-2 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01
ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher
concentrations for sizes of 500 µg and above.
Bioactive Protein: This IL-15
protein is biologically active
with an ED50 of 0.120 to 0.170
570502 570504 570506 570508
25 μg 100 μg 500 μg
10 μg
$225
$125
$550
$1600
Please request a quote for amounts greater than 500 µg.
Human IL-17A/F Heterodimer
Interleukin-17A/F (IL-17A/F) is part of the IL-17 cytokine family
which consists of six structurally related proteins (IL-17A, B, C, D,
Toll Free 1.877.BIOLEGEND (246.5343)
Recombinant Human IL-17A/F Heterodimer
Optical Density (450 nm)
Recombinant Human hIL-17A/F
is a disulfide linked heterodimer
consisting of N-terminal methionylated hIL-17A and non-methionylated hIL-17F.
Bioactive Protein: This IL-17A/F
protein was tested by induction
of IL-6 in human skin fibroblasts
by IL-17A/F. The ED50 is 15–25 ng/
ng/mL
mL, corresponding to a specific
Induction of IL-6 in
human skin fibroblast by
activity of 4 to 6.6 × 104units/mg.
IL‑17A/F heterodimer
Endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at
higher concentrations for sizes of 500 µg and above.
Bioactive Protein (Carrier-Free)
580602 580604 580606 580608
25 μg 100 μg 500 μg
10 μg
$385
$285
$1350 $5600
Please request a quote for amounts greater than 500 µg.
Human IL-17F
Other Name: ML-1
Interleukin-17F (IL-17F) is one of the six IL-17 family members (IL17A through IL-17F), that shares the closest homology (~50%
amino acid identity) to IL-17A. Like IL-17A, IL-17F has also been associated to autoimmune diseases. IL-17F is produced and secreted
by Th17 cells and activated monocytes. Like IL-17A, IL-17F has also
been linked to multiple biological activities on a variety of cells
including: inhibition of angiogenesis, induction of IL-2, IL-6, IL-8,
G-CSF, GM-CSF, GROα, ENA78, TGFβ, MCP1, and ICAM1 production in epithelial cells, endothelial cells, and fibroblasts. IL-17F and
IL-17A often work in coexpression manner. Recent studies have
demonstrated that coexpression of IL-17F and IL-17A in HEK293
cells results in the formation of biologically active IL-17A/F heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found
to produce the IL-17A/ F heterodimer, along with the corresponding homodimers. Among these, IL-17A homodimer was most potent, followed by IL-17A/ F heterodimer, then IL-17F homodimer
(100fold lower than IL-17A).
Recombinant Human IL-17F
Applications: Bioassay
Bioactive Protein: This IL-17F protein is biologically active
with an ED50 of 400 to 800 ng/mL, corresponding to a specific
activity of 1.25 to 2.5 × 103 units/mg as determined by
dose-­dependent stimulation of CXCL1 production in human
fibroblasts. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
570606
100 μg
$595
Human IL-22
Other Name: IL-TIF
Bioassay-Validated Recombinant Cytokines
Applications: Bioassay
Recombinant Human IL-17F (Arg 31–Gln 163, Accession #
AF384857) was expressed in E. coli and purified to greater than
98% purity.
Interleukin-22 (IL-22) was originally called IL-10-related T-cell-derived inducible factor (IL-TIF) and is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22,
IL-24 and IL-26. IL-22 is produced by activated NK and T-cells, particularly Th1 and Th17 cells. Th17 cells express IL-17A, IL-17F, IL-21
and IL-22, but are differentially regulated. Along with IL-17A and
IL-17F, IL-22 regulates genes associated with innate immunity of
the skin. IL-22 inhibits IL-4 production by Th2 cells, activates the
transcription factors STAT-1 and STAT-3 in several hepatoma cell
lines, and induces acute phase reactants in the liver and pancreas.
Recombinant human IL-22 is a 33.6 kD non-disulfide-linked homodimeric, non-glycosylated protein containing two 146 amino
acid polypeptide chains.
Recombinant Human IL-22
Applications: Bioassay
Recombinant Human IL-22 (Ala
34–Ile 179, Accession #
NM_020525) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (450 nm)
E, and F). IL-17A is expressed primarily by Th17 cells, a subset of
CD4+ T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% amino acid sequence homology. Like IL-17A,
IL-17F mRNA and protein have been detected in Th17 cells. L-17F
and IL-17A exist as a homodimers, adopting a cysteine knot motif
formed through the interactions of four cysteines, one of which
is responsible for interchain bonding. IL-17F and IL-17A can form
both homodimeric and heterodimeric proteins when expressed
in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. In addition, activated human
CD4+ T cells produce the homodimers of IL-17F, IL-17A, and the IL17A/F heterodimer.
Bioactive Protein: This IL-22
protein is biologically active
with an ED50 of 0.062 to 0.177
Induction of human
IL‑10 in human Colo 205
ng/mL, corresponding to a
cells by human IL-22
specific activity of 0.56 to 1.61
× 107 units/mg, as determined by a dose dependent stimulation
of human Colo205 cell production of IL-10. Endotoxin is less
than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes
10–100 µg and at higher concentrations for sizes of 500 µg and
above.
pg/mL
Bioactive Protein (Carrier-Free)
571302
10 μg
$195
571304
25 μg
$395
571306
100 μg
$900
571308
500 μg
$2500
Please request a quote for amounts greater than 500 µg.
Human IL-23
Interleukin-23 (IL-23) is a member of the IL-6 family of cytokines,
comprised of two subunits, p19 and p40. The p19/p40 heterodimer is stabilized by a disulfide bond. The p40 subunit is shared
by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells; p40 is not expressed by these cells.
Therefore, the availability of functional IL-23 is limited by
Continues
biolegend.com • [email protected]
449
Human IL-23 continued
the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rβ1 and IL-23R. IL-23 activates Janus kinase
(JAK)/signal transducer and activator of transcription (STAT) signaling molecules. JAK2 is constitutively associated with the IL-23R
chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.
Optical Density (450 nm)
Recombinant human IL-23
consists of two subunits linked
via a disulfide bond: P19
(Accession# NP_057668: Ala21
- Pro189) and P40 (Accession#
NP_002178.2: Ile 23-Ser 328).
This IL-23 protein was expressed in insect cells, and
purified to greater than 95%
purity.
Bioactive Protein: This IL-23 protein is biologically active
with an ED50 = 0.2 to 0.8 ng/mL, corresponding to a specific
activity of 1.25 to 5 × 106 units/mg as determined by mouse
splenocyte IL-17A secretion, which is induced by hIL-23 in a
dose-dependent manner. This protein is bottled carrier-free at
200 µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
574102
10 μg
$275
Human IL-34 shares a sequence identity of 99.6%, 72% and 71%
with chimpanzee, rat, and mouse IL-34, respectively, on the amino
acid level. The IL-34 gene is syntenic in the human, chimpanzee,
rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology
with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity or signal activation.
IL-34 and CSF show an equivalent ability to support cell growth
or survival. However, these cytokines have differing ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells,
and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding
in the receptor to IL-34 and CSF and, as a result, different bioactivities and signal activation kinetics/strength are produced for
these cytokines.
Recombinant Human IL-34
Human IL-27
Applications: Bioassay
Human IL-34 (Accession#
NM_152456.2: Met1 - Pro242
with a C-terminal 8His tag) was
expressed CHO cells, and
purified to greater than 95%
purity.
Other Names: IL-27p28, IL-27B (EBI3)
Interleukin-27 (IL-27) is a heterodimeric cytokine that consists of
EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related
polypeptide. IL-27 is in the IL-6/IL-12 superfamily of cytokines
which also includes IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common
four-helix bundle and their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1
and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27–specific receptor chain, gp130 is the common receptor subunit of IL-6 type
cytokines. IL-27 is expressed at sites of inflammation in cytokinedriven autoimmune/inflammatory diseases, such as rheumatoid
arthritis, psoriasis, inflammatory bowel disease, and sarcoidosis.
Optical Density (450 nm)
Applications: Bioassay
ng/mL
Inhibition of IL-2 production
by human IL-27 in
activated splenocytes
Bioactive Protein: ED50 = 9–40
ng/mL, corresponding to a
specific activity of 0.25 to 1.1
× 105 units/mg, as determined by monocyte cell proliferation
induced by IL-34 in a dose dependent manner. Endotoxin is less
than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL
for sizes 10–100 µg.
ng/mL
Monocyte cell proliferation
induced by human IL-34.
Bioactive Protein (Carrier-Free)
577902 577904 577906
10 μg
25 μg 100 μg
$190
$350
$875
Recombinant Human IL-27
450
Human IL-34
Proliferation (%)
Bioassay-Validated Recombinant Cytokines
Applications: Bioassay
ng/mL
Bioactive Protein (Carrier-Free)
589202 589204
2 μg
100 μg
$95
$1100
Recombinant Human IL-23
Mouse splenocytes
IL-17A secretion induced
by human IL-23
Bioactive Protein: This IL-27 protein is biologically active with
an ED50 = 60 to 90 ng/mL, corresponding to a specific activity of
1.1 to 1.7 × 104 units/mg, as determined by IL-2 inhibition induced
by hIL-27 in mouse splenocytes activated with anti-CD3 and
anti-CD28 antibodies. The protein is bottled carrier-free at 100
µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
The human IL-27 consists of two
subunits linked via a 4x(GGGS)
bridge: Human CD33 signal
peptide (Met1-Ala16) human
EBV-3 induced gene 3 (EBI3)
(Accession# NM_005755 Arg21
– Lis229) and human p28
(Accession# NM_145659 Phe29
- Pro243) 10 His-tag. This
protein was purified to greater
than 95% purity.
Please request a quote for amounts greater than 500 µg.
Human MCP-1 (CCL2)
Other Names: MCAF, JE, SCYA2, HC-11, P6, SMC-CF
Monocyte chemotactic protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF), was identified
based on its ability to chemoattract monocytes. Subsequently,
MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical
to the product of the JE gene, a PDGF inducible gene. MCP-1 is a
member of the beta (C-C) chemokine subfamily known as CCL2.
Toll Free 1.877.BIOLEGEND (246.5343)
Recombinant Human MCP-1 (CCL2)
Recombinant human MCP-1 is an 8.6 kD protein containing 76
amino acids.
Applications: Bioassay
Chemotaxis index
Recombinant Human MCP-1
(Gln 24–Thr 99, Accession #
NM_002982) was expressed in
E. coli and purified to greater
than 98% purity.
Bioactive Protein (Carrier-Free)
571402 571404 571406 571408
25 μg 100 μg 500 μg
10 μg
$215
$125
$595
$1500
Bioactive Protein (Carrier-Free)
577302 577304 577306 577308
25 μg 100 μg 500 μg
10 μg
$225
$125
$720
$1800
Please request a quote for amounts greater than 500 µg.
Human RANTES (CCL5)
Other Names: sisδ, TCP228
RANTES is a 7.8 kD member of the CC family of chemokines also
known as CCL5, which is expressed in T-cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T-cells, and eosinophils, and induces histamine
release from basophils. In solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities
to the dimeric form. Aggregates cause T-cell and neutrophil activation while promoting HIV infection, whereas the dimeric form
exhibits RANTES-induced chemotaxis and inhibits HIV infection.
Interestingly, as T-cells become activated, RANTES secretion appears to decrease.
Please request a quote for amounts greater than 500 µg.
Human PDGF-BB
Recombinant Human PDGF-BB
Applications: Bioassay, Blocking
Proliferation/control
Recombinant Human PDGF-BB,
amino acids Ser82-Thr190
(NM_033016) was expressed in
E. coli.and purified to greater
than 98% purity.
Bioactive Protein: This
PDGF-BB protein is biologically
active with an ED50 of 10 to
20 ng/mL, corresponding to a
Recombinant Human RANTES
(Ser24-Ser91, Accession #
NM_002985) was expressed in
E. coli and purified to greater
than 98% purity.
Chemotaxis Index
PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five
members, PDGF-AA, PDGF-BB, PDGF-AB, PDGFCC, and PDGF-DD.
PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs).
PDGF CC and DD are secreted as full-length, latent dimers, and the
proteolytic removal of a CUB domain is required for the growth factor domain of PDGF CC or DD to activate the PDGF receptors. PDGFBB exerts its biological functions through the activation of dimeric
receptors made up of two structurally similar protein-tyrosine kinase
receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both
sheep and humans and also for canine tracheal myocytes. In cutaneous remodelling, studies ex vivo revealed that PDGF-BB is a mitogen
and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/
PDGF are thought to contribute to a number of human diseases,
especially malignancies. Autocrine signalling as a consequence of
PDGF-B overexpression is clearly implicated in the pathogenesis of
dermatofibrosarcoma protruberans (DFSP).
Proliferation of 3T3 cells
induced by human PDGF-BB
Recombinant Human RANTES/CCL5
Applications: Bioassay
Other Names: Simian sarcoma viral oncogene
homolog, V-SIS, SSV, SIS, C-SIS, PDGF2
ng/mL
Bioassay-Validated Recombinant Cytokines
Bioactive Protein: This MCP-1
protein is biologically active
THP-1 cell chemotaxis
with an ED50 of 6 to 15 ng/mL,
induced by human MCP-1
corresponding to a specific
activity of 0.6 to 1.6 × 105 units/mg, as determined by the dose
dependent chemoattraction of THP-1 cells. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
ng/mL
specific activity of 0.5 to 1 × 105 units/mg, as determined by the
dose dependent stimulation of 3T3 cell proliferation. Endotoxin
is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein: This
RANTES protein is biologically
active with an ED50 of 5 to 15
ng/mL, corresponding to a specific activity of 0.66 to 2.0 × 105
units/mg. Bioactivity was measured by its property to chemoattract T cells in a dose dependent manner. Endotoxin is less than
0.1 EU/µg (<0.01 ng/µg).
ng/mL
Chemotactic activity
of human RANTES
Bioactive Protein (Carrier-Free)
580202 580204 580206 580208
25 μg 100 μg 500 μg
10 μg
$225
$125
$600
$1600
Please request a quote for amounts greater than 500 µg.
Human SDF-1α (CXCL12)
Other Names: Stromal cell deriver factor 1, SCYB12,
PBSF
Human SDF-1 belongs to the CXCL chemokine family. The mouse
cDNA SDF was initially cloned from a bone marrow stromal cell
library, and the human was cloned from a pro-B-cell cDNA library.
SDF is expressed by many organs, and it is most abundantly expressed in pancreas, spleen, ovary, and small intestine. SDF-1
(CXCL12) and its receptor CXCR4 are involved in regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+
stem cells. Stress-induced modulation in SDF-1 and CXCR4 levels
participate in recruitment of immature and maturing leukocytes
from the bone marrow reservoir to damaged organs as part of
host defense and repair mechanism. SDF-1 (CXCL12)/CXCR4 Continues
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451
Human SDF-1α continued
Recombinant Human SDF-1α (CXCL12)
Recombinant Human SDF-1α
(Lys22-Lys89, Accession #
NM_199168) was expressed in
E. coli and purified to greater
than 98% purity.
Chemotaxis Index
Bioassay-Validated Recombinant Cytokines
Applications: Bioassay
Bioactive Protein: This SDF-1α
protein is biologically active
Human resting T cells were
with an ED50 of 80 to 120 ng/
chemoatracted by SDF-1α
mL, corresponding to a specific
activity of 0.83 to 1.25 × 104 units/mg. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
581202
10 μg
$175
581204
25 μg
$325
581206
100 μg
$895
581208
500 μg
$2100
Please request a quote for amounts greater than 500 µg.
Human TGF-β1
Other Names: CED, DPD1
TGF-β1 is synthesized in cells as a 390-amino acid protein. Furin
cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide
(LAP) and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. In addition to furin, some other activators can release TGF-β from LAP. TGF-β activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen
species, and the integrins αvβ6 and αvβ8. Mouse TGF-β converts
naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. However, human TGF-β1 seems not to take part in T reg
differentiation. Murine Th17 response can be induced from naïve
CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct.
TGF-β1 seems to have dual effects on human Th17 differentiation
in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood,
TGF-β1 can inhibit the function of RORγt at high doses. By using
serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2
in combination with IL-6, IL-21 or IL-23.
Recombinant Human TGF-β1
Applications: Bioassay
Optical Density (490 nm)
Recombinant Human TGF-β1
(Ala279-Ser390, Accession #
NM_000660) was expressed in
CHO cells. Mature TGF-β1 was
purified from the conditioned
media.
Bioactive Protein: TGF-β1
inhibits the proliferation of
mouse HT-2 cells induced by
IL-4. The ED50 is 0.05 to 0.2 ng/mL, corresponding to a specific
activity of 0.5 to 2.0 × 107 units/mg. Endotoxin is less than 0.1
EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg
and at higher concentrations for sizes of 500 µg and above.
ng/mL
TGFβ1 inhibits the proliferation
of TH-2 cells induced by IL-4
Bioactive Protein (Carrier-Free)
580702 580704 580706 580708
25 μg 100 μg 500 μg
10 μg
$385
$285
$1600 $5000
Please request a quote for amounts greater than 500 µg.
Human TNF-α
Other Names: TNFSF2, TNF, MCF, Cachectin,
Necrosin, Macro­phage cytotoxic factor, DIF
TNF-α is secreted by macrophages, monocytes, neutrophils, Tcells (principally CD4+), and NK-cells. Many transformed cell lines
also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid
protein (non-glycosylated) with a reported molecular weight of
17 kD. TNF-α forms multimeric complexes; stable trimers are most
common in solution. A 26 kD membrane form of TNF-α has also
been described. TNF-α binding to surface receptors elicits a wide
array of biologic activities, including cytolysis and cytostasis of
many tumor cell lines in vitro, hemorrhagic necrosis of tumors in
vivo, increased fibroblast proliferation, and enhanced chemotaxis
and phagocytosis in neutrophils.
Recombinant Human TNF-α
Applications: Bioassay
Recombinant Human TNF-α
(Val 77–Leu 233, Accession #
NM_000594) was expressed in
E. coli and purified to greater
than 98% purity.
Optical Density (490 nm)
system is involved in the establishment of organ metastasis in different cancers, for example in lymph node metastasis in breast
cancer and oral squamous cell carcinoma (SCC), and peritoneal
metastasis in ovarian cancer. Recently, several studies have demonstrated the existence of a small subset of cancer cells which
share many characteristics with stem cells and named cancer stem
cells (CSC). They constitute a reservoir of self-sustaining cells with
the ability to maintain the tumor growth. Most of them express
CXCR4 receptor and respond to a chemotactic gradient of its specific ligand SDF-1, suggesting that CSC probably represent a subpopulation capable of initiating metastasis.
Bioactive Protein: This TNF-α
protein is biologically active
Cytotoxic effect of human
with an ED50 of 0.020 to 0.050
TNFa in L929 cells
ng/mL, corresponding to a
specific activity of 2 to 5 × 107 units/mg, as determined by dose-­
dependent stimulation of L929 cells treated with actinomycin
D. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at
200 µg/mL for sizes 10–100 µg and at higher concentrations for
sizes of 500 µg and above.
ng/mL
Bioactive Protein (Carrier-Free)
570102
10 μg
$125
570104
50 μg
$200
570106
100 μg
$360
570108
500 μg
$800
Please request a quote for amounts greater than 500 µg.
452
Toll Free 1.877.BIOLEGEND (246.5343)
Human TNF-β
Human VEGF-165
Other Names: LT-α, Coley’s toxin, Hemorrhagic
factor, Necrosin, NKCF, DIF, TNFSF-1
Other Name: Vascular permeability factor (VPF)
Tumor necrosis factor-beta (TNF-β), also known as lymphotoxin-α
(LT-α) is a potent lymphoid factor that exerts cytotoxic effects on
a wide range of tumor cells and certain other target cells. TNF-β
possesses a signal peptide sequence and is a secreted protein;
however, TNF-β is also present on the surface of activated T, B
and LAK cells as a complex with LT-beta. Bioactive TNF-β exists
as a homotrimer.
Recombinant Human TNF-β
Recombinant human TNF-β is an 18.6 kD protein containing 171
amino acids.
Bioactive Protein: This TNF-β protein is bioactive and can be
used for in vitro assays.
Bioactive Protein
562603
5 μg
$125
Human TWEAK (CD255)
TWEAK (TNFSF12) is a “TNF-like weak inducer” of apoptosis
through a non-death domain-dependent mechanism. TWEAK is
a type II membrane protein which exhibits a single internal hydrophobic domain of 27 amino acids in the N-terminal region. TWEAK
is proteolytically cleaved to produce a soluble cytokine that signals as a trimerized molecule. Fibroblast growth factor-inducible
14 (Fn14)/TWEAKR has been described as a receptor for TWEAK,
and it is associated with proliferation of endothelial cells and angiogenesis. However, TWEAK mediates signal transduction and
linear differentiation of monocyte/macrophage cells lacking Fn14/
TWEAKR, suggesting that such cells contain an alternative TWEAK
receptor. Elevated levels of TWEAK and/or Fn14 have been found
to be associated with the pathogenesis of rheumatoid arthritis,
skeletal muscle wasting, systemic lupus erythematosus, multiple
sclerosis, stroke, neuroinflammation and neurodegeneration, and
several types of cancer. The pathological functions of TWEAK are
primarily attributed to its ability to induce the expression of several proinflammatory cytokines, chemokines, cell adhesion molecules, and matrix-degrading enzymes mainly through the activation of NF-B, a major proinflammatory transcription factor. More
recently, it has been described that CD163 (a scavenger receptor)
might act as a receptor “decoy” for the ligand TWEAK.
Recombinant Human VEGF-165
Applications: Bioassay
Recombinant human VEGF-165, amino acids Ala27-Arg191 (Accession# NM_001171626) was expressed in E. coli. This protein
was purified to greater than 95% purity.
Bioactive Protein: This VEGF-165 protein is biologically active
with an ED50 = 2.5 to 4.5 ng/mL, corresponding to a specific
activity of 2.2–4 × 105 units/mg, as determined by HUVEC
cell proliferation in a dose dependent manner. The protein
is provided lyophilized, and endotoxin is less than 0.1 EU/µg
(<0.01 ng/µg).
Bioactive Protein
584501 584502
2 μg
100 μg
$75
$385
Bioassay-Validated Recombinant Cytokines
Applications: ELISA, Bioassay
VEGF-165 plays a critical role in new blood vessel formation in
vivo. VEGF-165 induces angiogenesis on different levels: it acts
as mitogen especially on endothelial cells, raises the vessel permeability and dilation by releasing NO, and has chemotactic impact on monocytes/macrophages, which plays a crucial role in
inducing inflammatory neovascularization. Angiogenic activities
of VEGFs are mediated primarily through two receptors, VEGFR-1
and VEGFR-2. VEGFR-2 signaling is enhanced by interactions with
co-receptors such as heparin/heparan sulfate and Neuropilin 1
(NRP-1). The expression of VEGF-165 in the tumor microenvironment influences the differentiation of bone marrow-derived pericytes to the Ewing’s sarcoma vasculature. VEGF induces osteoclast
migration and activation. In addition, it supports the survival of
mature osteoclasts. These processes play a key role in repairing
and remodeling bone during bone development. VEGF is upregulated by hypoxia and by overexpression of oncogenes such as
mutant ras and c-myc in tumours. Immobilized forms of perlecan
and PlnDI (inmovilized and soluble) bind VEGF-165 to coordinate
developmental angiogenesis modulating VEGF-165/VEGFR-2 signaling. In addition, CXCL8/IL-8 stimulates VEGF expression and
VEGFR2 activation in endothelial cells.
Recombinant Human TWEAK
Applications: Bioassay
Recombinant Human TWEAK (Lis97-His249, Accession
#NM_003809.2) was expressed in E. coli and purified to greater
than 98% purity.
Bioactive Protein: This TWEAK protein is biologically active
with an ED50 < 10 ng/mL, corresponding to a specific activity
of > 1 × 107 units/mg, as determined by the dose dependent
stimulation of production of IL-8 by human PBMC. Bottled
lyophilized, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg).
Bioactive Protein (Carrier-Free)
566402
5 μg
$125
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453