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Bioassay-Validated Recombinant Cytokines 432 432 432 432 433 433 433 434 434 434 434 435 435 435 436 436 436 437 437 437 438 438 438 439 Mouse IL-33 ������������������������������������������������� 439 Mouse IL-34 ������������������������������������������������� 439 Mouse Isthmin1 ����������������������������������������� 440 Mouse MCP-1 (CCL2) �������������������������������� 440 Mouse SCF ��������������������������������������������������� 440 Mouse TACI-Fc Chimera ������������������������� 441 Mouse TNF-α ����������������������������������������������� 441 Mouse TRANCE (RANKL) ������������������������� 441 Mouse VEGF120 ������������������������������������������� 442 Rat IL-2 ����������������������������������������������������������� 442 Rat IL-6 ����������������������������������������������������������� 442 Rat TNF-α ����������������������������������������������������� 443 Human CXCL9 (MIG) ��������������������������������� 443 Human FGF-basic/145 aa ����������������������� 443 Human G-CSF ��������������������������������������������� 443 Human GM-CSF ����������������������������������������� 444 Human IFN-γ ����������������������������������������������� 444 Human IL-1α ������������������������������������������������� 444 Human IL-1β ������������������������������������������������� 444 Human IL-2 ��������������������������������������������������� 445 Human IL-3 ��������������������������������������������������� 445 Human IL-4 ��������������������������������������������������� 445 Human IL-5 ��������������������������������������������������� 446 Human IL-6 ��������������������������������������������������� 446 biolegend.com • [email protected] Human IL-7 ��������������������������������������������������� 446 Human IL-8 ��������������������������������������������������� 446 Human IL-10 ������������������������������������������������� 447 Human IL-12 (p70) ������������������������������������� 447 Human IL-13 ������������������������������������������������� 448 Human IL-15 ������������������������������������������������� 448 Human IL-17A ��������������������������������������������� 448 Human IL-17A/F Heterodimer ��������������� 448 Human IL-17F ����������������������������������������������� 449 Human IL-22 ������������������������������������������������ 449 Human IL-23 ������������������������������������������������ 449 Human IL-27 ������������������������������������������������� 450 Human IL-34 ����������������������������������������������� 450 Human MCP-1 (CCL2) ������������������������������� 450 Human PDGF-BB ��������������������������������������� 451 Human RANTES (CCL5) ��������������������������� 451 Human SDF-1α (CXCL12) ������������������������� 451 Human TGF-β1 ������������������������������������������� 452 Human TNF-α ��������������������������������������������� 452 Human TNF-β ��������������������������������������������� 453 Human TWEAK (CD255) ��������������������������� 453 Human VEGF-165 ��������������������������������������� 453 Bioassay-Validated Recombinant Cytokines Mouse CXCL9 (MIG) ��������������������������������� Mouse FGF-basic ��������������������������������������� Mouse GM-CSF ������������������������������������������� Mouse IFN-γ ����������������������������������������������� Mouse IL-1α ������������������������������������������������� Mouse IL-1β ������������������������������������������������� Mouse IL-2 ��������������������������������������������������� Mouse IL-3 ��������������������������������������������������� Mouse IL-4 ��������������������������������������������������� Mouse IL-5 ��������������������������������������������������� Mouse IL-6 ��������������������������������������������������� Mouse IL-7 ��������������������������������������������������� Mouse IL-10 ������������������������������������������������� Mouse IL-12 (p70) ��������������������������������������� Mouse IL-12/IL-23 p40 (homodimer) ��� Mouse IL-13 ������������������������������������������������� Mouse IL-15 ������������������������������������������������� Mouse IL-17A ����������������������������������������������� Mouse IL-17A/F Heterodimer ����������������� Mouse IL-17F ����������������������������������������������� Mouse IL-22 ������������������������������������������������� Mouse IL-23 ������������������������������������������������� Mouse IL-25 (IL-17E) ����������������������������������� Mouse IL-27 ������������������������������������������������� 431 Mouse CXCL9 (MIG) Other Name: SCYB9 CXCL9 is an inflammatory chemokine initially identified by differential screening of a cDNA library from limphokine-activated macrophages. CXCL9 is a CXC chemokine and member of the nonELR (lacking a Glu-Leu-Arg motif in the N-terminal region) CXC chemokine family. CXCL9 shares the CXCR3 receptor with CXCL10 and CXCL11, and these ligands differ in their receptor-binding and activating properties; CXCL11 is more potent than CXCL10, and CXCL10 is more potent than CXCL9. Please request a quote for amounts greater than 500 µg. Mouse CXCL9, amino acids Thr22 - Thr126 (Accession # NM_008599), was expressed in E. coli, and purified to greater than 98% purity. T cells chemoattracted by mouse CXCL9 Bioactive Protein: Bioactivity was measured by its property to chemoattract human T cells activated with PHA and IL-2 in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) 578202 578204 578206 578208 25 μg 100 μg 500 μg 10 μg $225 $125 $595 $2000 Please request a quote for amounts greater than 500 µg. Other Names: CSF-α, Pluripoietin-α, Eo-CSF, BPA GM-CSF (granulocyte/macrophage-colony stimulating factor) is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells. Recombinant Mouse GM-CSF Applications: Bioassay Recombinant Mouse GM-CSF (Ala 18–Lys 141, Accession #NM_009969) was expressed in E. coli and purified to greater than 98% purity. Optical Density (450nm) ng/mL Bioactive Protein (Carrier-Free) 579602 579604 579606 579608 25 μg 100 μg 500 μg 10 μg $225 $125 $550 $1600 Mouse GM-CSF Application: Bioassay Chemotaxis Index Bioassay-Validated Recombinant Cytokines Recombinant Mouse CXCL9 (MIG) Bioactive Protein: This FGF-basic protein is biologically active with an ED50 of 0.3 to 2 ng/mL, corresponding to a specific activity of 0.5 to 3.3 × 106 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein: This GM-CSF protein is biologically MC/9 cell proliferation active with an ED50 of 0.05 ng/ induced by mouse GM-CSF. mL (2 × 107 units/mg). Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Mouse FGF-basic Other names: Fibroblast growth factor 2, Fgf-2, Fgfb, bFGF FGF-basic (FGFb) is a member of the fibroblast growth factor (FGF) family which includes 23 members. It is also known as fibroblast growth factor 2, Fgf-2, Fgfb, and bFGF. FGFb is expressed in almost all tissues and plays an important role in a variety of normal and pathological processes, including development, wound healing, and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. It shows potent angiogenic activity and has been implicated in tumor angiogenesis. FGFb also significantly promoted the proliferation of adipose-derived mesenchymal cells (AMC) and enhanced chondrogenesis in three-dimensional micromass culture. FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4. In addition, FGFb binds to the extracellular matrix and heparan sulfate (HS), which is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface, although there is controversy regarding the exact manner by which this occurs. Bioactive Protein (Carrier-Free) 576302 576304 576306 576308 25 μg 100 μg 500 μg 10 μg $225 $125 $700 $1700 Please request a quote for amounts greater than 500 µg. Mouse IFN-γ Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophageactivating factor, MAF Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts antiproliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Recombinant Mouse IFN-γ Recombinant Mouse FGF-basic Applications: Bioassay Applications: Bioassay Recombinant Mouse FGF-basic (Ala11-Ser154, Accession # NM_008006) was expressed in E. coli and purified to greater than 98% purity. 432 Toll Free 1.877.BIOLEGEND (246.5343) Bioactive Protein: This IFN-γ protein is biologically active Inhibition of WEHI-279 with an ED50 = 0.2 to 0.6 ng/ cell proliferation induced mL, corresponding to a specific by mouse IFN-γ activity of 1.67 to 5 × 106 units/ mg, as determined by a inhibition of WEHI-279 cell proliferation induced by mouse IFN-γ. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Interleukin-1β (IL-1β) is synthesized as a 31 kD precursor that is proteolytically cleaved by IL-1β converting enzyme to generate the released ~17 kD bioactive IL-1β. IL-1β is a potent pro-inflammatory cytokine and induces many responses, including activation of B and T cells. IL-1β binds to IL-1 receptor type 1 which contains the intracellular signaling domain(s) and to the IL-1 receptor type II which lacks the intracellular signaling domain. The IL-1 receptor type II is also shed from cells and binds and neutralizes IL-1β. Recombinant Mouse IL-1β Applications: Bioassay Please request a quote for amounts greater than 500 µg. Mouse IL-1α Other Names: IL-1F1, LAF, EP, LEM, MCF/MNCF Interleukin-1α (IL-1α) is synthesized as a 31 kD precursor that is proteolytically cleaved by IL-1β converting enzyme to generate the released ~17 kD bioactive IL-1α. IL-1α is a potent pro-inflammatory cytokine and induces many responses in many cell types including lymphocytes, fibroblasts, and epithelial cells. IL-1α binds to IL-1 receptor type 1 which contains the intracellular signaling domain(s) and to the IL-1 receptor type II which lacks the intracellular signaling domain. The IL-1 receptor type II is also shed from cells and binds and neutralizes IL-1α. Recombinant Mouse IL-1α Recombinant Mouse IL-1β (Val 118–Ser 269, Accession #NM_008361) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control 575302 575304 575306 575308 25 μg 100 μg 500 μg 10 μg $195 $125 $300 $600 Bioactive Protein: This IL-1β protein is biologically active D10S cell proliferation with an ED50 of 0.001 to 0.005 induced by mouse IL-1β ng/mL, corresponding to a specific activity of 0.2 to 1 × 109 units/mg, as determined by the dose dependent stimulation of D10S cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. pg/mL Bioactive Protein (Carrier-Free) 575102 10 μg $175 575104 25 μg $295 575106 100 μg $895 575108 500 μg $2100 Please request a quote for amounts greater than 500 µg. Applications: Bioassay Recombinant Mouse IL-1α (Ser 115–Ser 270, Accession # NM_010554) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control Other Names: H1, IFNβ-inducing factor, IL-β, OAF, Catabolin Bioassay-Validated Recombinant Cytokines Bioactive Protein (Carrier-Free) Mouse IL-1β Bioactive Protein: This IL-1α protein is biologically active Proliferation of DS10 cells with an ED50 of 1 to 5 pg/mL, induced by mouse IL-1α corresponding to a specific activity of 0.2 to 1 × 109 units/mg, as determined by the dose dependent stimulation of D10S cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. pg/mL Bioactive Protein (Carrier-Free) 575002 575004 575006 575008 25 μg 100 μg 500 μg 10 μg $295 $175 $795 $2100 Please request a quote for amounts greater than 500 µg. Mouse IL-2 Other Names: TCGF, EDF, KHF, MAF-C I, TDF Interleukin-2 (IL-2) homodimer is secreted by T-cells and binds to high affinity receptor IL-2Rα/β/γ (α = CD55, β = CD122, γ = CD132) and lower affinity IL-2Rβ/γ and (IL-2Rα)2. IL-2 induces proliferation and/or differentiation of activated helper T-cells, tumor infiltrating lymphocytes, macrophages, natural killer cells, oligodendrocytes and antibody producing B-cells. IL-2 influences expansion and maintenance of CD25+FOXP3+CD4+ T-regulatory cells and thus affects anti-tumor responses. Recombinant Mouse IL-2 Applications: Bioassay Recombinant Mouse IL-2 (Ala 21–Gln 169, Accession #NM_008366) was expressed in E. coli and purified to greater than 98% purity. Optical Density 490 nm Optical Density (450nm) Recombinant Mouse IFN-γ (His 23–Cys 155, Accession # NM_008337) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-2 protein is biologically active HT-2 cell proliferation with an ED50 of 0.10 to 0.40 ng/ induced by mouse IL-2 mL, corresponding to a specific activity of 2.5 to 10 × 106 units/mg, as determined by dose- dependent stimulation of HT-2 cells. Endotoxin is less than Continues ng/mL biolegend.com • [email protected] 433 Mouse IL-2 continued 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein (Carrier-Free) 575402 575404 575406 575408 25 μg 100 μg 500 μg 10 μg $225 $125 $500 $1400 Please request a quote for amounts greater than 500 µg. Interleukin-3 (IL-3) is produced by activated T cells, mast cells, eosinophils, keratinocytes, and astrocytes. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and bone marrow-derived macrophages. IL-3 induces proliferation and differentiation of hematopoietic stem cells and lineage committed progenitor cells. IL-3 effects on target cells can be multi-faceted and are often influenced by other cytokines or growth factors. Recombinant Mouse IL-3 Applications: Bioassay, ELISA Recombinant Mouse IL-3 (Ala 27–Cys 166, Accession #NM_010556) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-3 protein is biologically active ng/mL Bioactivity of mouse IL-3 with an ED50 of 0.100 ng /mL, was tested by a proliferation corresponding to a specific assay using M-NFS-60 cells. activity of 1 × 107 units/mg, as determined by the dose-dependent stimulation of a M-NFS-60 cell proliferation assay. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Proliferation/control Bioactive Protein (Carrier-Free) 574308 500 μg $2000 Please request a quote for amounts greater than 500 µg. Mouse IL-5 Other Names: EDF, Eo-CSF, BCGF-2, BCDF-m, IgA-EF, TRF-1 Interleukin-5 (IL-5) is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35 to 37 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors and stimulates the activation and differentiation of eosinophils, and promotes the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B cells and stimulates the production and secretion of IgM and IgA by B cells stimulated with bacterial endotoxin. Recombinant Mouse IL-5 Applications: ELISA, Bioassay Bioactive Protein: This IL-5 protein is bioactive and can be used for in vitro assays. Bioactive Protein 563303 5 μg $210 Other Names: Interferon-β2, BSF-2, CDF, HSF, HPGF Other Names: BSF-1, IaIF, HCGF, MCGF-2, MFT, TCGF-2 Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. Recombinant Mouse IL-4 Applications: Bioassay Proliferation/control 574306 100 μg $875 Mouse IL-6 575502 575504 575506 575508 25 μg 100 μg 500 μg 10 μg $295 $175 $900 $2200 Mouse IL-4 434 574304 25 μg $345 Mouse IL-4, amino acids His23-Ser140 (Accession# NM_021283), was expressed in 293E cells using human IL-2 signal peptide, and purified to greater than 95% purity. Bioactive Protein: This IL-4 protein is biologically active Interleukin-6 (IL-6) is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Recombinant Mouse IL-6 Applications: Bioassay, ELISA Recombinant Mouse IL-6 (Phe 25–Thr 211, Accession # NM_031168) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control Bioassay-Validated Recombinant Cytokines Other Names: BP/BPA, Eo-CSF, HCGF, MGF/MCGF, Multi-CSF, PCSA, Thy1 inducing factor ng/mL Bioactive Protein (Carrier-Free) 574302 10 μg $185 Mouse IL-3 CTLL-2 proliferation induced by mouse IL-4 with an ED50 = 2 to 4 ng/mL, corresponding to a specific activity of 2.5 to 5 × 105 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. pg/mL 7TD1 cell proliferation induced by mouse IL-6. Bioactive Protein: This IL-6 protein is biologically active with an ED50 < 0.01 ng/mL, corresponding to a specific activity of > 1 × 108 units/mg, as determined by the dose- Toll Free 1.877.BIOLEGEND (246.5343) Bioactive Protein (Carrier-Free) 575702 10 μg $125 575704 575706 575708 25 μg 100 μg 500 μg $225 $550 $1600 Please request a quote for amounts greater than 500 µg. Mouse IL-7 Other Names: Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor In adult mice, IL-7/IL-7R signaling up-regulates expression of early B cell factor (EBF), which in turn, regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. IL-7 also plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces anti-apoptotic factors Bcl2 and Bcl-xL and inhibits pro-apoptotic factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo. CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGF-β induces IL-7 downregulation. Recombinant Mouse IL-7 Application: Bioassay Proliferation/control Mouse IL-7, amino acids Glu26 -Ile154 (Accession # NM_008371.4) was expressed in insect cells, and purified to greater than 95% purity. Bioactive protein: ED50 = 1.0 to 2.5 ng/mL, corresponding to a ng/mL PHA activated PBL proliferation specific activity of 0.4 to 1 × 106 induced by mouse IL-7 units/mg, as determined by PHA-activated PBL proliferation induced by IL-7 in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes up to 500 µg and at higher concentrations for greater amounts. Other Names: CSIF, B-TCGF, TGIF Interleukin-10 (IL-10) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. Recombinant Mouse IL-10 Applications: Bioassay, ELISA Recombinant Mouse IL-10 (Ser 19–Ser 178 (Cys167Tyr), Accession #NM_010548) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-10 protein is biologically active with an ED50 of 1 to 2 ng/mL, corresponding to a specific activity of 0.5 to 1 × 106 units/mg, as determined by the dose-dependent stimulation of MC/9 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein (Carrier-Free) 575802 575804 575806 575808 25 μg 100 μg 500 μg 10 μg $295 $175 $595 $2100 Please request a quote for amounts greater than 500 µg. Mouse IL-12 (p70) Other Names: NKSF, TSF Interleukin-12 [IL-12 (p70)] is a disulfide-linked heterodimer composed of unrelated p40 (glycosylated) and p35 subunits. IL-12 acts as a growth factor for activated human T and NK cells, enhances the lytic activity of human NK cells, and stimulates the production of IFN-γ by resting human PBMC. IL-12R is formed by two chains, IL-12Rβ1 and IL-12Rβ2. IL-12Rβ1 is associated with the Janus kinase (Jak) Tyk2 and binds IL-12 p40; IL-12Rβ2 is associated with Jak2 and binds either the heterodimer or the p35 chain. Signaling through the IL-12 receptor complex induces phosphorylation, dimerization, and nuclear translocation of several signal transducer and activator of transcription (STAT) family members (STAT1, 3, 4, 5), but most of the biological responses to IL-12 have been attributed to STAT4. Recombinant Mouse IL-12 (p70) Applications: Bioassay ng/mL Mouse splenocytes IFN-γ production induced by mouse IL-12. Bioactive Protein (Carrier-Free) 577802 577804 10 μg 25 μg $185 $325 Please request a quote for amounts greater than 500 µg. Bioassay-Validated Recombinant Cytokines Interleukin-7 (IL-7) was initially described as a pre B-cell growth factor expressed in bone marrow stromal cells. IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of T-cells. The binding of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc), which leads to activation of receptorassociated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The activated JAK proteins, in turn, phosphorylate specific residues on the IL-7R, creating docking sites for signaling molecules such as STAT5, and to a lesser extent, STAT1 and STAT3. Mouse IL-10 OD (450–570nm) dependent stimulation of 7TD1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Recombinant mouse IL-12 (p70) heterodimer: P40 (Accession # NM_008352: Met23-Ser335) and P35 (Accession # NM_008351: Arg23-Ala215). This protein was expressed in Sf9 cells as secreted protein, and purified to greater than 95% purity. Bioactive Protein: This IL-12 protein is biologically active with an ED50 = 0.10 to 0.20 ng/mL, corresponding to a specific activity of 0.5 to 1.0 × 107 Continues biolegend.com • [email protected] 435 Mouse IL-12 (p70) continued units/mg, as determined by the dose-dependent stimulation of IFN-γ production by mouse splenocytes. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) 577002 577004 577006 577008 25 μg 100 μg 500 μg 10 μg $350 $195 $1200 $3500 Please request a quote for amounts greater than 500 µg. Mouse IL-12/IL-23 p40 (homodimer) Mouse IL-13 Other Names: ALRH, BHR1, P600 Interleukin-13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. Recombinant Mouse IL-13 Applications: Bioassay Recombinant Mouse IL-13 (Ser 26–Phe 131, Accession #NM_008355) was expressed in E. coli and purified to greater than 98% purity. IL-12 and IL-23 share the p40 subunit, which heterodimerizes with IL-12 p35 or IL-23 p19 subunits, respectively, to form IL-12 or IL-23. IL-12 p40 exists as a monomer and as a homodimer (IL-12 p80). IL-12 induction is relevant in asthmatic airway inflammation and IL-12 expression can be induced by mouse parainfluenza type I (Sendai) virus in airway epithelial cells. In that experimental model, IL-12 induction is followed by excessive expression of IL-12 p40 that could be further enhanced in IL-12 p35-deficient mice. Overexpression of IL-12 p80 causes macrophage accumulation and contributes to airway inflammation and consequent morbidity during viral bronchitis. Amplified epithelial IL-12 p40 expression and augmented concentrations of BAL fluid IL-12 p40 (but not IL-12 p70) has been detected in asthmatic subjects. Also, it has been demonstrated that p80, but not IL-12 or p40, induces macrophage chemotaxis that is independent of IL-12 and mediated through the cytoplasmic tail of IL-12b1. Additional studies with transgenic mice suggest that overexpression of IL-12 p80 prior to a viral infection increases the number of resident airway macrophages, which primes the host for a protective response against a lethal respiratory viral infection. In addition, it has been suggested that p80 functions as a competitive antagonist of IL-12 p70. Mouse Con A-activated splenocytes display identical binding affinities for p80 and IL-12, and in these cells p80 competitively inhibited IL-12 binding and IL-12-dependent proliferation. Furthermore, p80 is able to inhibit IL-12-dependent IFN-γ production in freshly isolated splenocytes. Recombinant Mouse IL-12 (p40 homodimer) Applications: Bioassay OD (450–570nm) Recombinant Mouse IL-12 p40 homodimer, amino acids Met23-Ser335 (Accession # NM_008352), was expressed in insect cells and purified to greater than 98% purity. ng/mL Bioactive Protein: This IL-12 p40 protein is biologically active with an ED50 = 1 to 4 ng/mL corresponding to a specific activity of 0.25 to 1.0 × 106 units/mg, as determined by the dose dependent inhibition of IL-12-dependent IFNγ production. Bottled carrier-free at 100 µg/ mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Mouse IL-12 p40 homodimer is able to inhibit IL-12dependent IFNγ production. Bioactive Protein (Carrier-Free) 573102 436 10 μg $175 Proliferation/control Bioassay-Validated Recombinant Cytokines Other Name: p40 homodimer (p80) Bioactive Protein: This IL-13 protein is biologically active ng/mL with an ED50 of 1.5 to 3.5 ng/ TF-1 cells proliferation mL, corresponding to a specific induced by mouse IL-13. activity of 2.85 to 6.6 × 105 units/mg, as determined by the dose-dependent stimulation of TF-1 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein (Carrier-Free) 575902 575904 575906 575908 25 μg 100 μg 500 μg 10 μg $350 $175 $1100 $2500 Please request a quote for amounts greater than 500 µg. Mouse IL-15 Interleukin-15 (IL-15) was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL2. IL-15 is a regulatory cytokine, produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation. Recombinant Mouse IL-15 Applications: Bioassay Toll Free 1.877.BIOLEGEND (246.5343) Bioactive Protein: This IL-15 protein is biologically active CTLL-2 cell proliferation with an ED50 = 2.5 to 7.5 ng/ induced by mouse IL-15 mL, corresponding to a specific activity of 1.3 to 4 × 105 units/mg, as determined by the dose-dependent stimulation of CTLL-2 cell proliferation. Bottled lyophilized, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). ng/mL Bioactive Protein (Carrier-Free) Mouse IL-17A Other Names: IL-17, CTLA-8 Interleukin-17A (IL-17A) is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts. In addition, IL-17A enhances the surface expression of ICAM-1 in fibroblasts, activation of NF-κb, and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Mouse IL-17A shows 87%, 63% and 57% sequence identity to rat IL-17A, human IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively. Both recombinant and natural IL-17A have been shown to exist as a disulfide-linked homodimeric glycoprotein containing two ~16 kD monomers. Recombinant Mouse IL-17A Applications: ELISA, Bioassay Optical Density (450nm) Recombinant Mouse IL-17A (Ala26-Ala158, Accession #NM_010552) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-17A protein is biologically active with an ED50 of 0.5 to 2 ng/mL, corresponding to a specific activity 0.5–2 × 106 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Mouse IL-6 induced by mouse IL-17A in fetal mouse skin fibroblasts. Bioactive Protein (Carrier-Free) 576002 576004 576006 576008 25 μg 100 μg 500 μg 10 μg $225 $125 $500 $1500 Please request a quote for amounts greater than 500 µg. Interleukin-17A/F (IL-17A/F) is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% amino acid sequence homology. Like IL-17A, IL17F mRNA and protein have been detected in Th17 cells. IL-17F and IL-17A exist as homodimers, adopting a cystine knot motif formed through the interactions of four cystines, one of which is responsible for the interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. Mouse Th17 cells secrete IL17A/F as well as the homodimers IL-17A and IL-17F. IL-17A/F induces IL-6 and KC (CXCL1) production in mouse fibroblasts and macrophages. IL-17A/F signals through IL-17RA and IL-17RC. Bioassay-Validated Recombinant Cytokines 566301 566304 2 μg 100 μg $75 $895 Mouse IL-17A/F Heterodimer Recombinant Mouse IL-17A/F Heterodimer Applications: Bioassay Optical Density (450nm) Proliferation/control Recombinant Mouse IL-15 (Asn49-Ser162, Accession #NM_008357) was expressed in E. coli and purified to greater than 95% purity. ng/mL Recombinant Mouse IL-17A (Ala26–Ala158, Accession # NM_010552) and mouse IL-17F, amino acids Arg 29–Ala 161 (Accession #NM_145856) were expressed in E. coli and purified to greater than 95% purity. IL-6 induced by IL-17A/F in fetal mouse fibroblast Bioactive Protein: This IL-17A/F protein is biologically active with an ED50 = 15 to 50 ng/mL, corresponding to a specific activity 2.0 to 6.7 × 104 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6. Bottled lyophilized with no additives, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 580802 580804 580806 580808 25 μg 100 μg 500 μg 10 μg $385 $195 $1000 $3000 Mouse IL-17F Interleukin-17F (IL-17F) belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50% amino acid sequence homology. Recently, an IL-17–IL-17F heterodimer was found to be expressed in Th17 cells, together with IL-17 and IL17F homodimers. IL-17 and IL-17F gene promoters share the same pattern of chromatin remodeling in differentiated Th17 cells. T cell populations in vitro and in vivo have shown different ratios of IL-17 and IL-17F expression. IL-17, IL-17F regulates proinflammatory gene expression in vitro. Studies in vivo using Il17a(-/-), Il17f(-/-), and Il17a(-/-)Il17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collageninduced arthritis, and arthritis in Il1rn(-/-) mice. In contrast, both IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation. Continues biolegend.com • [email protected] 437 Mouse IL-17F continued Mouse IL-23 Recombinant Mouse IL-17F Application: Bioassay Bioactive protein: ED50 = 300 to 600 ng/mL, corresponding to CXCL1 induced by mouse IL-17F a specific activity of 0.16 to 0.33 in fetal mouse skin fibroblasts × 104 units/mg, as determined by dose-dependent stimulation of fetal mouse skin fibroblasts production of CXCL1. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled carrier-free at 200 µg/mL up to 100 µg size and at higher concentrations for greater amounts. Bioassay-Validated Recombinant Cytokines ng/mL Bioactive Protein (Carrier-Free) 576106 100 μg $550 Recombinant Mouse IL-23 Applications: Bioassay 576108 500 μg $1550 Please request a quote for amounts greater than 500 µg. Mouse IL-22 Other Names: IL-TIF ng/mL Interleukin-22 (IL-22) was originally named IL-10-related T cell-derived inducible factor (IL-TIF) and is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. IL-22 is produced by activated NK and T cells, particularly Th1 and Th17 cells. Th17 cells express IL-17A, IL-17F, IL-21 and IL-22, but they are differentially regulated. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-22 inhibits IL-4 production by Th2 cells, activates the transcription factors STAT-1 and STAT-3 in several hepatoma cell lines, and induces acute phase reactants in the liver and pancreas. Recombinant mouse IL-22 is a 33.4 kD non-disulfide-linked homodimeric, non-glycosylated protein containing two 146 amino acid polypeptide chains. Recombinant Mouse IL-22 Applications: Bioassay Recombinant Mouse IL-22 (Leu 34–Val 179, Accession #NM_016971) was expressed in E. coli and purified to greater than 98% purity. Optical Density (450nm) Interleukin-23 (IL-23) is a member of the IL-6 family of cytokines and consists of two subunits, p19 and p40. The p19-p40 heterodimer is stabilized by a disulfide bond. The subunit p40 is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells; nevertheless, p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rβ1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription signaling molecules (STAT). JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5. Optical Density (450nm) Optical Density (450nm) Mouse IL-17F, amino acids Arg29-Ala161 (Accession # NM_145856) was expressed in E. Coli, and purified to greater than 98% purity. Bioactive Protein: This IL-22 protein is biologically active Mouse IL-22 induces IL-10 with an ED50 of 0.095 to 0.265 in human Colo205 cells. ng/mL, corresponding to a specific a ctivity of 0.38 to 1.05 × 107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. pg/mL Bioactive Protein (Carrier-Free) Induction of mouse IL-17A in splenocytes by mouse IL-23 Recombinant mouse IL-23 consists of two subunits linked via a disulphide bond: P19 (Accession# NM_031252: Met1-Ala 196) and P40 (Accession# NM_008352 : Met Met1-Ser 335). Mouse IL-23 was expressed in insect cells, and purified to greater than 95% purity. Bioactive Protein: This IL-23 protein is biologically active with an ED50 = 0.5 to 0.8 ng/mL, corresponding to a specific activity of 1.25 to 2.0 × 106 units/mg, as determined by mouse splenocytes IL-17A secretion induced by mouse IL-23 in a dosedependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) 589002 589004 589006 589008 25 μg 100 μg 500 μg 10 μg $575 $275 $1800 $5400 Please request a quote for amounts greater than 500 µg. Mouse IL-25 (IL-17E) Interleukin-25 (IL-25), also known as IL-17E, is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F and IL-17A/F. Primary sequence homology differs between family members, with hIL-17 and hIL-17F having the highest homology (44%), while hIL17 and hIL-25 have the lowest (15%). IL-25 induces elevated gene expression of IL-4, IL-5, and IL-13 in multiple tissues and results in T helper 2 (TH2)-type immune responses (increased serum IgE levels) and pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, increased mucus production, epithelial cell hyperplasia and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B. Recombinant Mouse IL-25 (IL-17E) Applications: Bioassay 576202 576204 576206 576208 25 μg 100 μg 500 μg 10 μg $385 $195 $1200 $2950 Please request a quote for amounts greater than 500 µg. 438 Toll Free 1.877.BIOLEGEND (246.5343) Mouse IL-25 binding to recombinant IL17BR/Fc Chimera detected by functional ELISA Bioactive Protein: Mouse IL-25 binds to recombinant mouse IL17BR/Fc Chimera in a functional ELISA. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) Please request a quote for amounts greater than 500 µg. Mouse IL-27 Other Names: Interleukin 27, IL-27p28 (IL-30), EBI3 Interleukin IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 P40-related protein) and P28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/ IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization, and IFN-γ production in synergy with IL-12. The IL-27 induced Th1 differentiation was mediated by rapid and marked up-regulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 has an anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+ T cell and NK cell activation and a potential therapeutic role for autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27. Recombinant Mouse IL-27 Optical Density (450nm) Applications: Bioassay ng/mL Inhibition of IL-2 production induced by mouse IL-27 in mouse activated splenocytes. Recombinant mouse IL-27 consists of human CD33 signal peptide (Met1-Ala16), mouse EBI3 (NM_015766, Tyr19Pro228), and mouse p28 (NM_145636, Phe29-Ser234) joined via a GGGSGGGSGG GTGGGS linker, expressed in 293E cells. This protein was purified to greater than 95% purity. Bioactive Protein: This IL-27 protein is biologically active with an ED50 = 5 to 10 ng/mL, corresponding to a specific activity of 1.0 to 2.0 × 105 units/mg, as determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies. The protein is bottled carrier-free, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Please request a quote for amounts greater than 500 µg. Mouse IL-33 Other Names: Il-1f11, Nuclear factor from high endothelial venules, NF-HEV Interleukin-33 (IL-33) belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. These cytokines are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as the mature active form. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signaling events to those observed for IL-1b. IL-33 is a nuclear factor (NF-HEV) that is abundantly expressed in high endothelial venules (HEV) lymphoid tissues, and some epithelial cells. It associates with chromatin and exhibits transcriptional regulatory properties. Bioassay-Validated Recombinant Cytokines 579802 579804 579806 579808 25 μg 100 μg 500 μg 10 μg $300 $195 $600 $2000 Bioactive Protein (Carrier-Free) 577402 577404 577406 577408 25 μg 100 μg 500 μg 10 μg $395 $245 $1200 $4000 Recombinant Mouse IL-33 Applications: Bioassay Recombinant Mouse IL-33 (Ser109 - Ile266, Accession # NM_133775) was expressed in E. coli and purified to greater than 98% purity. IL-5 (pg/mL) Optical Density (450nm) mIL-25 (ng/mL) Recombinant mouse IL-25, amino acids Val17-Ala169 (Accession # NM_080729) was expressed in E. coli, and purified to greater than 98% purity. Bioactive Protein: This IL-33 protein is biologically active IL-5 induction by mouse IL-33 with an ED50 of 2 to 5 ng/mL, in splenocytes activated by corresponding to a specific anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew. activity of 2 to 5 × 105 units/mg, as determined by induction of IL-5 in activated splenocytes by IL-33. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) 580502 580504 580506 580508 25 μg 100 μg 500 μg 10 μg $325 $175 $800 $2200 Please request a quote for amounts greater than 500 µg. Mouse IL-34 Other Name: C16orf77 homolog Mouse IL-34 shares a sequence identity of 71% with human IL-34 on the amino acid level, and the IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have different ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF. Continues biolegend.com • [email protected] 439 Mouse IL-34 continued As a result, different bioactivities and signal activation kinetics/ strength are produced for these cytokines. Bioactive Protein (Carrier-Free) Recombinant Mouse IL-34 577502 Proliferation/Control Recombinant mouse IL-34 (Accession# NM_001135100.1: Met1–Pro235 with a C-terminal 8His tag) was expressed CHO cells, and purified to greater than 98% purity. Bioactive Protein: This IL-34 protein is biologically active M-NSF-60 cell proliferation with an ED50 = 20 to 30 ng/mL, induced by mouse IL-34. corresponding to a specific activity of 3.3 to 5 × 104 units/mg, as determined by M-NFS-60 cell proliferation induction in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioassay-Validated Recombinant Cytokines ng/mL Bioactive Protein (Carrier-Free) 577602 577604 577606 577608 25 μg 100 μg 500 μg 10 μg $595 $275 $1500 $4200 Please request a quote for amounts greater than 500 µg. 10 μg $265 Mouse MCP-1 (CCL2) Other Names: MCAF, JE, SCYA2, HC-11, P6, SMC-CF Monocyte chemotactic protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF), was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily known as CCL2. Recombinant Mouse MCP-1 (CCL2) Applications: Bioassay Recombinant Mouse MCP-1 (Gln24-Arg96, Accession # NM_011333) was expressed in E. coli and purified to greater than 98% purity. Chemotaxis Index Applications: Bioassay Bioactive Protein: This MCP-1 protein is biologically active THP-1 cell chemotaxis with an ED50 of 8 to 15 ng/mL, induced by mouse MCP-1. corresponding to a specific activity of 0.6 to 1.25 × 105 units/mg, as determined by the dose dependent chemoattraction of THP-1 cells. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL. ng/mL Mouse Isthmin1 Other Names: Isthmin-1, Ism1 Isthmin is a secreted protein, initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98% identity between mouse and human, and 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved, with 99% identity between mouse and human; and 91% identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Recently it has been suggested that isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in presence of VEGF. Over-expression of Isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk. Recombinant Mouse Isthmin1 Applications: Bioassay Recombinant mouse Isthmin1 (Accession# NM_001126490, Met 1 - Tyr 454) was expressed in 293E cells. This protein was purified to greater than 95% purity. 440 The protein is bottled carrier-free at 200 µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 576502 576504 10 μg 25 μg $195 $350 Mouse SCF Other Names: Kit Ligand, KITLG, Mast cell growth factor, MGF, Steel factor, SLF SCF (KITL) is a hematopoietic growth factor which can synergize with a number of other cytokines to stimulate growth of hematopoietic progenitors in vitro and stimulates blood cell production in vivo. SCF is encoded by Sl (‘steel’), a gene critical to the development of several distinct cell lineages during embryonic life. KITL was identified as a soluble protein; nevertheless, the predicted amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from a transmembrane precursor. Two splice variants have been described for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of KITL induces the dimerization of the KIT molecule, followed by a change in the configuration of the intracellular domain and the autophosphorylation of the receptor, opening several docking sites for signal transduction molecules. Dysregulation of SCF–KI signaling and gain-of-function KIT mutations contributes to the genesis of many cancers, like acute myeloid leukemia, gastrointestinal stromal tumors, and mastocytosis. Toll Free 1.877.BIOLEGEND (246.5343) Applications: Bioassay Proliferation/Control Recombinant mouse SCF, amino acids Lys26-Ala189 (Accession# M59915), was expressed in E. coli, and purified to greater than 98% purity. ng/mL TF-1 cell proliferation induced by mouse SCF Bioactive Protein (Carrier-Free) 579702 579704 579706 579708 25 μg 100 μg 500 μg 10 μg $325 $175 $725 $1475 Please request a quote for amounts greater than 500 µg. Mouse TACI-Fc Chimera Other Names: TNFRSF13B, CD267 Transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) is a TNFR family member molecule and part of the so-called BAFF system. This includes two ligands: B cell-activating factor of the TNF family (BAFF) and a proliferationinducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C), TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17). BAFF binds the three receptors, while APRIL binds TACI and BCMA. TACI is constitutively expressed on naïve B cells and is upregulated on activated B cells. TACI levels are severely reduced in newborn mice B cells compared with those of adult mice, and newborn B cells do not secrete Igs when they are stimulated with BAFF or APRIL. This reduced TACI expression might be responsible for the irresponsiveness of newborn B cells to T cell independent type II antigens such as bacterial capsular polysaccharides. Recombinant Mouse TACI-Fc Chimera Applications: Bioassay Optical Density (490nm) Mouse TACI (Accession # NM_021349: Met1-Thr 129) - SRENLYFQG- Human IgG (Pro 100 –Lys300) was expressed 293E cells, and purified to greater than 98% purity. ng/mL Bioactive Protein: This TACI-Fc Chimera protein is biologically active with an ED50 = 5 – 10 ng/mL, corresponding to a specific activity of 1.0 to 2.0 × 105 units/mg, as determined by inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc in a dose-dependent manner. Bottled carrier-free at 200 µg/mL for sizes 10–500 µg, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc. Please request a quote for amounts greater than 500 µg. Mouse TNF-α Other Names: TNF, Cachectin, Necrosin, MCF, DIF, TNFSF-2 Tumor necrosis factor-α (TNF-α) is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes, and stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Bioassay-Validated Recombinant Cytokines Bioactive Protein: This SCF protein is biologically active with an ED50 = 15 ng/mL, corresponding to a specific activity of 6.7 × 104 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) 577702 577704 577706 577708 25 μg 100 μg 500 μg 10 μg $225 $125 $625 $1600 Recombinant Mouse TNF-α Applications: Bioassay Recombinant Mouse TNF-α (Leu 80–Leu 235, Accession #NM_013693) was expressed in E. coli and purified to greater than 98% purity. Optical Density (490nm) Recombinant Mouse SCF Bioactive Protein: This TNF-α protein is biologically active with an ED50 of 0.010 to 0.020 ng/mL, corresponding to a specific activity of 5 to 10 × 107 units/mg, as determined by a dose dependent cytotoxic effect in L929 cells treated with actinomycin D. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Cytotoxic effect of mouse TNF-α in L929 cells Bioactive Protein (Carrier-Free) 575202 575204 575206 575208 25 μg 100 μg 500 μg 10 μg $225 $125 $550 $1475 Please request a quote for amounts greater than 500 µg. Mouse TRANCE (RANKL) Other Names: TNFSF11, OPGL, ODF, CD254 The RANKL gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kD. RANKL is cleaved to produce a soluble form with biological activity. The shedding of membrane-bound RANKL appears to be mediated by expression of matrix metalloproteinase (MMP) 14 and ADAM10. Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore, RANKL shedding seems to be an important process that down-regulates local osteoclastogenesis. Alternatively, an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation, activation, and survival, which results in increased bone resorption. Binding of RANKL to its receptor RANK activates TNF receptor- associated factor Continues biolegend.com • [email protected] 441 Mouse TRANCE continued Recombinant Mouse TRANCE (RANKL) Applications: Bioassay Bioactive Protein: This TRANCE protein’s bioactivity was measured by its property to induce osteoclast differentiation in RAW264.7 cells in the absence of any cross-linking. The bioactivity is equivalent to competitors’ cytokines. The protein is bottled carrier-free at 200 µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 10 μg Other Names: TCGF, EDF, KHF, MAF-C I, TDF Interleukin-2 (IL-2) is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. Recombinant Rat IL-2 Applications: Bioassay Recombinant Rat IL-2 (Ala21Met153, Accession # NM_053836) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-2 protein is biologically active with CTLL-2 proliferation an ED50 of 0.102 to 0.295 ng/ induced by rat IL-2. mL, corresponding to a specific activity of 3.39 to 9.8 × 106 units/mg, as determined by the dose dependent stimulation of CTLL-2 cells. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL PBS for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. ng/mL $200 Mouse VEGF120 Other Names: Vegf, Vegf-a, Vpf VegfA belong to the VEGF family which has at least 7 members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venomderived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA, and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in the synovial tissues of RA patients. Recombinant Mouse VEGF120 Applications: Bioassay Recombinant Mouse VEGF120, (Ala27-Arg146, Accession # S38100) was expressed in E. coli and purified to greater than 98% purity Bioactive Protein: This VEGF120 protein is biologically active with an ED50 of 1 to 4 ng/mL, corresponding to a specific activity of 0.25 to 1 × 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL VegfA induces the proliferation of HUVEC cells Bioactive Protein (Carrier-Free) 580902 580904 580906 580908 25 μg 100 μg 500 μg 10 μg $325 $195 $800 $1800 Bioactive Protein (Carrier-Free) 579502 579504 579506 579508 25 μg 100 μg 500 μg 10 μg $225 $125 $600 $1200 Please request a quote for amounts greater than 500 µg. Rat IL-6 Other Names: Interferon-β2, BSF-2, CDF, HSF, HPGF IL-6 is a multifunctional cytokine with both differentiation and growth-promoting effects for a variety of target cell types. It is a B-cell stimulatory factor inducing terminal differentiation and high level antibody production in B lymphocytes, and it acts as a hybridoma/plasmacytoma growth factor. IL-6 stimulates the growth of hematopoietic stem cells and induces the synthesis of acute phase plasma proteins. In addition, IL-6 and TNF-α are involved in osteoclastogenesis and are responsible for bone resorption; both cytokines participate in the pathogenesis of osteoporosis. Also, IL-6 is systemically elevated in obesity and is a predictive factor in type 2 diabetes. Furthermore, IL-6 regulates pancreatic alpha cell mass expansion and plays a pivotal role in liver regeneration. Rat IL-6 has 92% and 68% identity to the mouse and human nucleotide sequence, respectively. Recombinant Rat IL-6 Applications: Bioassay Recombinant Rat IL-6 (Phe25Thr211, Accession # NM_012589) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control 577102 Proliferation/control Bioassay-Validated Recombinant Cytokines Recombinant mouse TRANCE, amino acids Lis158-Asp316 (Accession # NM_011613.3) was expressed in insect cells, with an Nterminal 9-His tag and IEGR-Xa sequence. This protein was purified to greater than 95% purity. Rat IL-2 Proliferation/control 6 (TRAF6), which is linked to downstream pathways including NFkB, c-jun N-terminal kinase (JNK) or Src. TRAF6 has been shown to be necessary for the differentiation of osteoclastic cells by enhancing Src kinase, essential for osteoclast function. Please request a quote for amounts greater than 500 µg. pg/mL 7TD1 cell proliferation induced by rat IL-6. 442 Bioactive Protein: This IL-6 protein is biologically active with an ED50 < 0.01 ng/mL, corresponding to a specific activity of > 1 × 108 units/mg, Toll Free 1.877.BIOLEGEND (246.5343) Bioactive Protein (Carrier-Free) 579902 579904 579906 579908 25 μg 100 μg 500 μg 10 μg $225 $125 $550 $1600 Please request a quote for amounts greater than 500 µg. Recombinant Human CXCL9 (MIG) Application: Bioassay Human CXCL9, amino acids Thr23-Thr125 (Accession# NM_002416.1), was expressed in E. coli, and purified to greater than 98% purity. Chemotaxis Index as determined by the dose dependent stimulation of 7TD1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein: Human CXCL9 is biologically active and chemoattracts human T cells activated with PHA and IL-2 in a dose-dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. ng/mL Human T cells chemoattracted by human CXCL9 Other Names: TNFSF2, TNF, MCF Cachectin, Necrosin, Macrophage cytotoxic factor, DIF Recombinant Rat TNF-α Applications: Bioassay, ELISA Optical Density (450nm) Recombinant Rat TNF-α (Leu80-Leu235, Accession # NM_012675) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This TNF-α protein is biologically active with an ED50 of 5 to 15 pg/mL, corresponding to a specific activity of 0.66 to 2 × 108 units/mg, as determined by a dose dependent cytotoxicity assay using L929 cells treated with actinomycin D. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Rat TNF-α cytotoxicity on L929 cells. Bioactive Protein (Carrier-Free) 580102 580104 580106 580108 25 μg 100 μg 500 μg 10 μg $295 $150 $750 $1800 Please request a quote for amounts greater than 500 µg. Human CXCL9 (MIG) Other Name: SCYB9 CXCL9 is a member of the α subfamily of chemokines that lacks the ELR domain. CXCL9 has been shown to be a chemoattractant for activated T lymphocytes and TIL, but not for neutrophils or monocytes. A chemokine receptor (CXCR3) specific for CXCL9 and IP10 has been cloned and shown to be highly expressed in IL-2 activated T lymphocytes. Bioassay-Validated Recombinant Cytokines TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells following their stimulation by bacterial lipopolysaccharide and cytokines (IL-2, GM-CSF, interferons). Many transformed cell lines also secrete TNF-α. Monomeric rat TNF-α is a 158 amino acid protein (N-glycosylated) with a reported molecular weight of 17.3 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Bioactive Protein (Carrier-Free) 578102 10 μg $125 578104 25 μg $225 578106 100 μg $595 578108 500 μg $2400 Please request a quote for amounts greater than 500 µg. Human FGF-basic/145 aa Other Names: bFGF, FGF-2, Heparin-binding growth factor Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. Recombinant Human FGF-basic/145 aa Applications: Bioassay Recombinant Human FGF-basic (Ala 144–Ser 288, Accession # NM_002006) was expressed in E. coli and purified to greater than 98% purity. Optical Density (490 nm) Rat TNF-α Bioactive Protein: This FGF-basic protein is biologi3NIH/3T3 cell proliferation cally active with an ED50 of 1 induced by human FGFb to 4 ng/mL, corresponding to a specific activity of 0.25 to 1 × 106 units/mg, as determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) 571502 10 μg $125 571504 25 μg $225 571506 100 μg $550 571508 500 μg $1600 Please request a quote for amounts greater than 500 µg. Human G-CSF Other Names: CSF-β, G-CSA, MGI-2, Pluripoietin Granulocyte-colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophilic Continues biolegend.com • [email protected] 443 Human G-CSF continued granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-α, or IFN-γ. Recombinant Human G-CSF Applications: ELISA, Bioassay Human G-CSF is an 18.7 kD protein containing 174 amino acids. Bioactive Protein: This G-CSF protein is bioactive and can be used for in vitro assays. Bioactive Protein: This IFN-γ protein is biologically active with an ED50 of 0.2–1 ng/mL, corresponding to a specific activity of 1 to 5 × 106 units/mg, as determined by dose-dependent stimulation of HT-29 cells. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled at 100 µg/mL PBS for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. 570202 570204 570206 570208 25 μg 100 μg 500 μg 10 μg $125 $70 $195 $600 $125 Human GM-CSF Please request a quote for amounts greater than 500 µg. Other Names: CSF-α, Eo-CSF, BPA, Pluripoietin-α Human IL-1α Granulocyte/macrophage-colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, Bcells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Other Names: LAF, EP, LEM, MCF/MNCF Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are the products of distinct genes, but are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. Recombinant Human IL-1α Recombinant Human GM-CSF Applications: Bioassay, ELISA Recombinant Human GM-CSF (Ala18–Glu144, Accession # NM_000758) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This GM-CSF protein is biologically TF-1 cell proliferation induced active with an ED50 of 0.10 to by human GM-CSF 0.30 ng/mL, corresponding to a specific activity of 0.33 to 1 × 107 units/mg, as determined by dose-dependent TF-1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) 572902 572903 572904 572905 25 μg 100 μg 500 μg 10 μg $225 $125 $695 $1600 Please request a quote for amounts greater than 500 µg. Recombinant Human IL-1α (Ser 113–Ala 271, Accession # NM_000575) was expressed in E. coli and purified to greater than 98% purity. Recombinant human IL-1α is an 18.0 kD protein containing 159 amino acids. Proliferation/control Applications: Bioassay Proliferation/control Bioassay-Validated Recombinant Cytokines 2 μg Recombinant Human IFN-γ (Gln 24–Gln 166, Accession # NM_000619) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein (Carrier-Free) Bioactive Protein (Carrier-Free) 561701 Applications: Bioassay Bioactive Protein: This IL-1α protein is biologically active with an ED50 of 0.58 to 1.45 pg/ mL, corresponding to a specific activity of 0.689 to 1.72 × 109 units/mg, as determined by the dose-dependent stimulation of D10 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. pg/mL D10S cell proliferation induced by human IL-1α Bioactive Protein (Carrier-Free) 570002 570004 570006 570008 25 μg 100 μg 500 μg 10 μg $395 $195 $900 $2100 Human IFN-γ Please request a quote for amounts greater than 500 µg. Other Names: Immune interferon, Type II interferon, T-cell interferon, MAF Human IL-1β Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted primarily by activated NK-cells and T-cells. Originally characterized based on anti-viral activities, IFN-γ also exerts antiproliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Recombinant Human IFN-γ Other Names: H1, OAF, Catabolin, IFNβ-inducing factor Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are the products of distinct genes, but are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses, including the activation of B and T-cells. Recombinant human IFN-γ is a 16.7 kD protein containing 143 amino acids. 444 Toll Free 1.877.BIOLEGEND (246.5343) Applications: Bioassay, ELISA Recombinant Human IL-1β (Ala117-Ser269, Accession # NM_000576) was expressed in E. coli and purified to greater than 98% purity. Recombinant human IL-1β is a 17.3 kD protein containing 153 amino acids. Bioactive Protein: This IL-1β protein is biologically active with an ED50 of 0.858 to 1.85 pg/mL, corresponding to a specific activity of 0.54 to 1.16 × 109 units/mg, as determined by the dose dependent stimulation of D10 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Please request a quote for amounts greater than 500 µg. Human IL-2 Other Names: TCGF, EDF, KHF, MAF-C I, TDF Interleukin-2 (IL-2) was discovered through its function as a T cell growth factor (TCGF), and plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of the foreign Ags by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signal pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells. IL-2Rα is an IL-2-specific receptor, IL-2Rβ is shared with IL-15, and the γc is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Recombinant Human IL-2 Applications: Bioassay Proliferation/control Human IL-2, amino acids Ala21-Thr153 (Accession # NM_000586), was expressed in insect cells, and purified to greater than 95% purity. Bioactive Protein: This IL-2 protein is biologically active with an ED50 = 0.175 to 0.8 ng/mL, corresponding to a specific activity of 1.25 to 5.71 × 106 units/mg, as determined by the dose-dependent stimulation of CTLL2 cell proliferation. Bottled carrier-free at 200 µg/mL for sizes 10–100 µg, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). ng/mL CTLL-2 cell proliferation induced by human IL-2 Bioactive Protein (Carrier-Free) 589102 589104 589106 10 μg 25 μg 100 μg $125 $225 $595 Please request a quote for amounts greater than 100 µg. Other Names: BP/BPA, Eo-CSF, HCGF, MGF/MCGF, Multi-CSF, PCSA, Thy1 inducing factor Interleukin-3 (IL-3) is a hematopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hematopoietic cells. IL-3 is the most potent growth factor for basophils, followed by GM-CSF and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and large and rapidly released amounts of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections, and since basophils play an important role in Th2 immune responses, IL-3 is a critical regulator of allergic inflammation. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development. Bioassay-Validated Recombinant Cytokines Bioactive Protein (Carrier-Free) 579402 579404 579406 579408 25 μg 100 μg 500 μg 10 μg $295 $175 $1100 $2100 Human IL-3 Recombinant Human IL-3 Application: Bioassay Human IL-3, amino acids His23-Ser140 (Accession# NM_021283), was expressed in sf9 cells with C-terminal His-8, and purified to greater than 95% purity. Proliferation/control Recombinant Human IL-1β Bioactive Protein: ED50 = 0.05 – 0.3 ng/mL, corresponding to a specific activity of 0.33 – 2 × 107 units/mg, as determined by the dose-dependent stimulation of TF-1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg. ng/mL TF-1 cell proliferation induced by human IL-3 Bioactive Protein (Carrier-Free) 578002 578004 578006 578008 25 μg 100 μg 500 μg 10 μg $295 $150 $495 $1175 Please request a quote for amounts greater than 500 µg. Human IL-4 Other Names: BCGF-1, BSF-1, LSF-1 Interleukin-4 (IL-4) is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. The Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is most active during airway hypersensitivity and mucus secretion and is not found in T cells. Continues biolegend.com • [email protected] 445 Human IL-4 continued Applications: Bioassay Bioactive Protein: This IL-4 protein is biologically active with an ED50 = 0.2 to 0.6 ng/ mL, corresponding to a specific activity of 1.65 to 5 × 106 units/ mg, as determined by the dose dependent stimulation of TF-1 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. ng/mL Bioassay-Validated Recombinant Cytokines TF-1 cell proliferation induced by human IL-4 Bioactive Protein (Carrier-Free) Human IL-7 Please request a quote for amounts greater than 500 µg. Human IL-5 Other Names: EDF, Eo-CSF, BCGF-2, BCDF-m, IgA-EF, TRF-1 Interleukin-5 (IL-5) is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Recombinant Human IL-5 Applications: ELISA, Bioassay Bioactive Protein: This IL-5 protein is bioactive and can be used for in vitro assays. Bioactive Protein 2 μg Bioactive Protein (Carrier-Free) 570802 570804 570806 570808 25 μg 100 μg 500 μg 10 μg $225 $125 $650 $1500 Please request a quote for amounts greater than 500 µg. 574002 574004 574006 574008 25 μg 100 μg 500 μg 10 μg $225 $125 $675 $1800 560701 Bioactive Protein: This IL-6 protein is biologically active 7TD1 cell proliferation with an ED50 of 4 to 6 pg/mL, induced by human IL-6 corresponding to a specific activity of 1.6 to 2.5 × 108 units/mg, as determined by a dose dependent stimulation in a 7TD1 cell proliferation assay. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL $125 Other Names: LP-1, Pre-BCGF, TGF Interleukin-7 (IL-7) is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. Recombinant Human IL-7 Applications: Bioassay Recombinant Human IL-7 (Asp26-His177, Accession # NM_000880) was expressed in E. coli and purified to greater than 98% purity. Optical Density (490 nm) Proliferation/control Recombinant Human IL-4, amino acids His25-Ser153 (Accession # NM_000589), was expressed in E. Coli, and purified to greater than 95% purity. Recombinant Human IL-6 (Pro 29–Met 212, Accession # NM_000600) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control Recombinant Human IL-4 Bioactive Protein: This IL-7 protein is biologically active Mouse IXN/2B cell proliferation with an ED50 <0.5 ng/mL, induced by human IL-7 corresponding to a specific activity of greater than 2.0 × 106 units/mg, as determined by the dose dependent stimulation of mouse IxN/2b cell proliferation. ng/mL Bioactive Protein (Carrier-Free) Human IL-6 566501 566503 2 μg 100 μg $95 $1100 Other Names: BSF-2, CDF, HSF, HPGF Interleukin-6 (IL-6) is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. Recombinant Human IL-6 Recombinant human IL-6 is a 26 kD protein containing 184 amino acids. Applications: Bioassay, ELISA 446 Human IL-8 Other Names: SCYB8, MDNCF, NAP-1, GCP1, CXCL8 Interleukin-8 (IL-8), also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Toll Free 1.877.BIOLEGEND (246.5343) Recombinant Human IL-8 Recombinant Human IL-10 (Mammalianexpressed) ng/mL Neutrophils Chemoattracted by Human IL-8 bioactivity Recombinant human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584) was expressed in E. coli, and purified to greater than 95% purity. Bioactive Protein (Carrier-Free) 574202 10 μg $125 574204 25 μg $225 574206 100 μg $580 574208 500 μg $1400 ng/mL Human IL-10 inhibits the production of IFNγ in PMA activated PBMC Bioactive Protein: This IL-10 protein is biologically active with an ED50 = 0.1 to 0.3 ng/mL corresponding to a specific activity of 3.3 to 10 × 106 units/mg, as determined by the dose dependent inhibition of IFN-γ production by PMA activated PBMC. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) Please request a quote for amounts greater than 500 µg. Human IL-10 573202 573204 573206 10 μg 25 μg 100 μg $195 $395 $1200 Please request a quote for amounts greater than 100 µg. Other Name: CSIF Interleukin-10 (IL-10) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. Recombinant Human IL-10 (E. Coli-expressed) Recombinant human IL-10 is an 18.6 kD protein containing 160 amino acids. Human IL-12 (p70) Other Names: NKSF, CLMF Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. Recombinant Human IL-12 (p70) Applications: Bioassay Recombinant Human IL-10 (Ser 19–Asn 178, Accession # NM_000572) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-10 protein is biologically active with an ED50 of 0.8 to 1.5 ng/ mL corresponding to a specific activity of 0.66 to 1.25 × 106 units/mg, as determined by the dose dependent stimulation of MC/9 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. ng/mL MC/9 cell proliferation induced by human IL-10 Bioactive Protein (Carrier-Free) 571002 571004 571006 571008 25 μg 100 μg 500 μg 10 μg $350 $175 $1100 $2500 Please request a quote for amounts greater than 500 µg. Optical Density (450nm) Applications: Bioassay Proliferation/control Recombinant Human IL-10, amino acids Ser19-Asn178 (Accession # NM_000572), was expressed in 293E cells, using human IL-2 signal peptide, and purified to greater than 95% purity. ng/mL IFNγ induced by human IL-12 in PBMC activated with PHA Bioassay-Validated Recombinant Cytokines Bioactive Protein: This IL-8 protein’s bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose-dependent manner. The highest chemotactic activity was detected from 10 to 100 ng/mL. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Applications: Bioassay Optical Density (450nm) Chemotaxis Index Applications: Bioassay Recombinant Human IL-12 (p70) consists of two subunits linked via a disulphide bond: P35 (Accession# NM_000882: Arg 57- Ser 253) and P40 (Accession# NM_002187: Ile 23-Ser 328). This IL-12 protein is expressed in insect cells as secreted protein and purified to greater than 95% purity. Bioactive Protein: This IL-12 protein is biologically active with an ED50 = 0.05 to 0.10 ng/mL, corresponding to a specific activity of 1.0 – 2.0 × 107 units/mg, as determined by the production of IFNγ by activated human PBMC in response to IL-12. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 100 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and higher. Bioactive Protein (Carrier-Free) 573002 573004 573006 573008 25 μg 100 μg 500 μg 10 μg $395 $195 $1200 $2600 Please request a quote for amounts greater than 500 µg. biolegend.com • [email protected] 447 Human IL-13 Other Names: NC30, ALRH, BHR1, P600 Interleukin-13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. Recombinant Human IL-13 Applications: Bioassay, ELISA Recombinant Human IL-13 (Gly 21–Asn 132, Accession # NM_002188) was expressed in E. coli and purified to greater than 98% purity. Proliferation/control Bioactive Protein: This IL-13 protein is biologically active ng/mL with an ED50 of 1.5 to 3 ng/mL, TF-1 cells proliferation corresponding to a specific induced by human IL-13 activity of 3.3 to 6.6 × 105 units/ mg, as determined by the dose dependent stimulation of TF-1 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein (Carrier-Free) 571102 10 μg $195 571104 25 μg $395 571106 100 μg $1200 Please request a quote for amounts greater than 500 µg. Human IL-17A Other Names: IL-17, CTLA-8 Interleukin-17A (IL-17A) is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial cells, endothelial cells and fibroblasts. In addition, IL-17A induces expression of ICAM in fibroblasts, activation of NF-κB, and costimulation of T-cell proliferation. Studies conducted in mice demonstrated that activated IL-17-secreting CD4+ helper T-cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL17A is closely related to a protein encoded by the ORF13 gene of herpesvirus saimiri (HVS13) with overall sequence identity of 72% at the amino acid level and 75% at the nucleotide level. The sequence homology between human IL-17A and mouse IL-17A is 63% at the amino acid level and 72% at the nucleotide level. Both recombinant and natural IL-17A have been shown to exist as a disulfide-linked homodimeric glycoprotein containing two ~16 kD monomers. Please request a quote for amounts greater than 500 µg. Other Name: MGC9721 Interleukin-15 (IL-15) is an immunomodulating cytokine that is secreted mainly by mononuclear phagocytes following infection by viruses. Human IL-15 shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. IL-15 shares many biological properties with IL-2 including stimulatory activities on the proliferation, survival, and activation of T lymphocytes and NK-cells. Recent reports have also suggested that that IL-15 plays important roles in the pathogenesis of rheumatoid arthritis via activation of T-cells and induction of IL-17 production. Recombinant human IL-15 is a ~13 kD single non-glycosylated polypeptide chain. Proliferation/control Recombinant Human IL-15 (Asn 49–Ser 162, Accession # NM_000585) was expressed in E. coli and purified to greater than 98% purity. ng/mL Recombinant Human IL-17A (Ile 20–Ala 155, Accession # NM_002190) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This IL-17A protein is biologically active Induction of IL-6 in human with an ED50 of 2 to 4 ng/mL, dermal fibroblast by IL-17A corresponding to a specific activity 2.5 to 5 × 105 units/mg, as determined by a dose dependent stimulation of normal human dermal fibroblasts production of IL-6. Endotoxin is less than 0.1 EU/µg (<0.01 ng/ µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) Applications: Bioassay, ELISA CTLL-2 cell proliferation induced by human IL-15 Recombinant Human IL-17A Applications: Bioassay, ELISA Human IL-15 Recombinant Human IL-15 448 Bioactive Protein (Carrier-Free) 570302 570304 570306 570308 25 μg 100 μg 500 μg 10 μg $395 $195 $1100 $2100 Optical Density (450 nm) Bioassay-Validated Recombinant Cytokines Recombinant human IL-13 is a 12.5 kD protein containing 114 amino acids. ng/mL, corresponding to a specific activity of 5.88 to 8.33 × 106 units/mg, as determined by the dose dependent stimulation of CTLL-2 cells proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein: This IL-15 protein is biologically active with an ED50 of 0.120 to 0.170 570502 570504 570506 570508 25 μg 100 μg 500 μg 10 μg $225 $125 $550 $1600 Please request a quote for amounts greater than 500 µg. Human IL-17A/F Heterodimer Interleukin-17A/F (IL-17A/F) is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, Toll Free 1.877.BIOLEGEND (246.5343) Recombinant Human IL-17A/F Heterodimer Optical Density (450 nm) Recombinant Human hIL-17A/F is a disulfide linked heterodimer consisting of N-terminal methionylated hIL-17A and non-methionylated hIL-17F. Bioactive Protein: This IL-17A/F protein was tested by induction of IL-6 in human skin fibroblasts by IL-17A/F. The ED50 is 15–25 ng/ ng/mL mL, corresponding to a specific Induction of IL-6 in human skin fibroblast by activity of 4 to 6.6 × 104units/mg. IL‑17A/F heterodimer Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. Bioactive Protein (Carrier-Free) 580602 580604 580606 580608 25 μg 100 μg 500 μg 10 μg $385 $285 $1350 $5600 Please request a quote for amounts greater than 500 µg. Human IL-17F Other Name: ML-1 Interleukin-17F (IL-17F) is one of the six IL-17 family members (IL17A through IL-17F), that shares the closest homology (~50% amino acid identity) to IL-17A. Like IL-17A, IL-17F has also been associated to autoimmune diseases. IL-17F is produced and secreted by Th17 cells and activated monocytes. Like IL-17A, IL-17F has also been linked to multiple biological activities on a variety of cells including: inhibition of angiogenesis, induction of IL-2, IL-6, IL-8, G-CSF, GM-CSF, GROα, ENA78, TGFβ, MCP1, and ICAM1 production in epithelial cells, endothelial cells, and fibroblasts. IL-17F and IL-17A often work in coexpression manner. Recent studies have demonstrated that coexpression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17A/F heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/ F heterodimer, along with the corresponding homodimers. Among these, IL-17A homodimer was most potent, followed by IL-17A/ F heterodimer, then IL-17F homodimer (100fold lower than IL-17A). Recombinant Human IL-17F Applications: Bioassay Bioactive Protein: This IL-17F protein is biologically active with an ED50 of 400 to 800 ng/mL, corresponding to a specific activity of 1.25 to 2.5 × 103 units/mg as determined by dose-dependent stimulation of CXCL1 production in human fibroblasts. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 570606 100 μg $595 Human IL-22 Other Name: IL-TIF Bioassay-Validated Recombinant Cytokines Applications: Bioassay Recombinant Human IL-17F (Arg 31–Gln 163, Accession # AF384857) was expressed in E. coli and purified to greater than 98% purity. Interleukin-22 (IL-22) was originally called IL-10-related T-cell-derived inducible factor (IL-TIF) and is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. IL-22 is produced by activated NK and T-cells, particularly Th1 and Th17 cells. Th17 cells express IL-17A, IL-17F, IL-21 and IL-22, but are differentially regulated. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-22 inhibits IL-4 production by Th2 cells, activates the transcription factors STAT-1 and STAT-3 in several hepatoma cell lines, and induces acute phase reactants in the liver and pancreas. Recombinant human IL-22 is a 33.6 kD non-disulfide-linked homodimeric, non-glycosylated protein containing two 146 amino acid polypeptide chains. Recombinant Human IL-22 Applications: Bioassay Recombinant Human IL-22 (Ala 34–Ile 179, Accession # NM_020525) was expressed in E. coli and purified to greater than 98% purity. Optical Density (450 nm) E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4+ T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% amino acid sequence homology. Like IL-17A, IL-17F mRNA and protein have been detected in Th17 cells. L-17F and IL-17A exist as a homodimers, adopting a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. In addition, activated human CD4+ T cells produce the homodimers of IL-17F, IL-17A, and the IL17A/F heterodimer. Bioactive Protein: This IL-22 protein is biologically active with an ED50 of 0.062 to 0.177 Induction of human IL‑10 in human Colo 205 ng/mL, corresponding to a cells by human IL-22 specific activity of 0.56 to 1.61 × 107 units/mg, as determined by a dose dependent stimulation of human Colo205 cell production of IL-10. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. pg/mL Bioactive Protein (Carrier-Free) 571302 10 μg $195 571304 25 μg $395 571306 100 μg $900 571308 500 μg $2500 Please request a quote for amounts greater than 500 µg. Human IL-23 Interleukin-23 (IL-23) is a member of the IL-6 family of cytokines, comprised of two subunits, p19 and p40. The p19/p40 heterodimer is stabilized by a disulfide bond. The p40 subunit is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells; p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by Continues biolegend.com • [email protected] 449 Human IL-23 continued the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rβ1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling molecules. JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5. Optical Density (450 nm) Recombinant human IL-23 consists of two subunits linked via a disulfide bond: P19 (Accession# NP_057668: Ala21 - Pro189) and P40 (Accession# NP_002178.2: Ile 23-Ser 328). This IL-23 protein was expressed in insect cells, and purified to greater than 95% purity. Bioactive Protein: This IL-23 protein is biologically active with an ED50 = 0.2 to 0.8 ng/mL, corresponding to a specific activity of 1.25 to 5 × 106 units/mg as determined by mouse splenocyte IL-17A secretion, which is induced by hIL-23 in a dose-dependent manner. This protein is bottled carrier-free at 200 µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 574102 10 μg $275 Human IL-34 shares a sequence identity of 99.6%, 72% and 71% with chimpanzee, rat, and mouse IL-34, respectively, on the amino acid level. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity or signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have differing ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF and, as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines. Recombinant Human IL-34 Human IL-27 Applications: Bioassay Human IL-34 (Accession# NM_152456.2: Met1 - Pro242 with a C-terminal 8His tag) was expressed CHO cells, and purified to greater than 95% purity. Other Names: IL-27p28, IL-27B (EBI3) Interleukin-27 (IL-27) is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related polypeptide. IL-27 is in the IL-6/IL-12 superfamily of cytokines which also includes IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common four-helix bundle and their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1 and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27–specific receptor chain, gp130 is the common receptor subunit of IL-6 type cytokines. IL-27 is expressed at sites of inflammation in cytokinedriven autoimmune/inflammatory diseases, such as rheumatoid arthritis, psoriasis, inflammatory bowel disease, and sarcoidosis. Optical Density (450 nm) Applications: Bioassay ng/mL Inhibition of IL-2 production by human IL-27 in activated splenocytes Bioactive Protein: ED50 = 9–40 ng/mL, corresponding to a specific activity of 0.25 to 1.1 × 105 units/mg, as determined by monocyte cell proliferation induced by IL-34 in a dose dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled carrier-free at 200 µg/mL for sizes 10–100 µg. ng/mL Monocyte cell proliferation induced by human IL-34. Bioactive Protein (Carrier-Free) 577902 577904 577906 10 μg 25 μg 100 μg $190 $350 $875 Recombinant Human IL-27 450 Human IL-34 Proliferation (%) Bioassay-Validated Recombinant Cytokines Applications: Bioassay ng/mL Bioactive Protein (Carrier-Free) 589202 589204 2 μg 100 μg $95 $1100 Recombinant Human IL-23 Mouse splenocytes IL-17A secretion induced by human IL-23 Bioactive Protein: This IL-27 protein is biologically active with an ED50 = 60 to 90 ng/mL, corresponding to a specific activity of 1.1 to 1.7 × 104 units/mg, as determined by IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies. The protein is bottled carrier-free at 100 µg/mL, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). The human IL-27 consists of two subunits linked via a 4x(GGGS) bridge: Human CD33 signal peptide (Met1-Ala16) human EBV-3 induced gene 3 (EBI3) (Accession# NM_005755 Arg21 – Lis229) and human p28 (Accession# NM_145659 Phe29 - Pro243) 10 His-tag. This protein was purified to greater than 95% purity. Please request a quote for amounts greater than 500 µg. Human MCP-1 (CCL2) Other Names: MCAF, JE, SCYA2, HC-11, P6, SMC-CF Monocyte chemotactic protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF), was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily known as CCL2. Toll Free 1.877.BIOLEGEND (246.5343) Recombinant Human MCP-1 (CCL2) Recombinant human MCP-1 is an 8.6 kD protein containing 76 amino acids. Applications: Bioassay Chemotaxis index Recombinant Human MCP-1 (Gln 24–Thr 99, Accession # NM_002982) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein (Carrier-Free) 571402 571404 571406 571408 25 μg 100 μg 500 μg 10 μg $215 $125 $595 $1500 Bioactive Protein (Carrier-Free) 577302 577304 577306 577308 25 μg 100 μg 500 μg 10 μg $225 $125 $720 $1800 Please request a quote for amounts greater than 500 µg. Human RANTES (CCL5) Other Names: sisδ, TCP228 RANTES is a 7.8 kD member of the CC family of chemokines also known as CCL5, which is expressed in T-cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T-cells, and eosinophils, and induces histamine release from basophils. In solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities to the dimeric form. Aggregates cause T-cell and neutrophil activation while promoting HIV infection, whereas the dimeric form exhibits RANTES-induced chemotaxis and inhibits HIV infection. Interestingly, as T-cells become activated, RANTES secretion appears to decrease. Please request a quote for amounts greater than 500 µg. Human PDGF-BB Recombinant Human PDGF-BB Applications: Bioassay, Blocking Proliferation/control Recombinant Human PDGF-BB, amino acids Ser82-Thr190 (NM_033016) was expressed in E. coli.and purified to greater than 98% purity. Bioactive Protein: This PDGF-BB protein is biologically active with an ED50 of 10 to 20 ng/mL, corresponding to a Recombinant Human RANTES (Ser24-Ser91, Accession # NM_002985) was expressed in E. coli and purified to greater than 98% purity. Chemotaxis Index PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGFCC, and PDGF-DD. PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs). PDGF CC and DD are secreted as full-length, latent dimers, and the proteolytic removal of a CUB domain is required for the growth factor domain of PDGF CC or DD to activate the PDGF receptors. PDGFBB exerts its biological functions through the activation of dimeric receptors made up of two structurally similar protein-tyrosine kinase receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both sheep and humans and also for canine tracheal myocytes. In cutaneous remodelling, studies ex vivo revealed that PDGF-BB is a mitogen and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/ PDGF are thought to contribute to a number of human diseases, especially malignancies. Autocrine signalling as a consequence of PDGF-B overexpression is clearly implicated in the pathogenesis of dermatofibrosarcoma protruberans (DFSP). Proliferation of 3T3 cells induced by human PDGF-BB Recombinant Human RANTES/CCL5 Applications: Bioassay Other Names: Simian sarcoma viral oncogene homolog, V-SIS, SSV, SIS, C-SIS, PDGF2 ng/mL Bioassay-Validated Recombinant Cytokines Bioactive Protein: This MCP-1 protein is biologically active THP-1 cell chemotaxis with an ED50 of 6 to 15 ng/mL, induced by human MCP-1 corresponding to a specific activity of 0.6 to 1.6 × 105 units/mg, as determined by the dose dependent chemoattraction of THP-1 cells. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL specific activity of 0.5 to 1 × 105 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein: This RANTES protein is biologically active with an ED50 of 5 to 15 ng/mL, corresponding to a specific activity of 0.66 to 2.0 × 105 units/mg. Bioactivity was measured by its property to chemoattract T cells in a dose dependent manner. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). ng/mL Chemotactic activity of human RANTES Bioactive Protein (Carrier-Free) 580202 580204 580206 580208 25 μg 100 μg 500 μg 10 μg $225 $125 $600 $1600 Please request a quote for amounts greater than 500 µg. Human SDF-1α (CXCL12) Other Names: Stromal cell deriver factor 1, SCYB12, PBSF Human SDF-1 belongs to the CXCL chemokine family. The mouse cDNA SDF was initially cloned from a bone marrow stromal cell library, and the human was cloned from a pro-B-cell cDNA library. SDF is expressed by many organs, and it is most abundantly expressed in pancreas, spleen, ovary, and small intestine. SDF-1 (CXCL12) and its receptor CXCR4 are involved in regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulation in SDF-1 and CXCR4 levels participate in recruitment of immature and maturing leukocytes from the bone marrow reservoir to damaged organs as part of host defense and repair mechanism. SDF-1 (CXCL12)/CXCR4 Continues biolegend.com • [email protected] 451 Human SDF-1α continued Recombinant Human SDF-1α (CXCL12) Recombinant Human SDF-1α (Lys22-Lys89, Accession # NM_199168) was expressed in E. coli and purified to greater than 98% purity. Chemotaxis Index Bioassay-Validated Recombinant Cytokines Applications: Bioassay Bioactive Protein: This SDF-1α protein is biologically active Human resting T cells were with an ED50 of 80 to 120 ng/ chemoatracted by SDF-1α mL, corresponding to a specific activity of 0.83 to 1.25 × 104 units/mg. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) 581202 10 μg $175 581204 25 μg $325 581206 100 μg $895 581208 500 μg $2100 Please request a quote for amounts greater than 500 µg. Human TGF-β1 Other Names: CED, DPD1 TGF-β1 is synthesized in cells as a 390-amino acid protein. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP) and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. In addition to furin, some other activators can release TGF-β from LAP. TGF-β activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and the integrins αvβ6 and αvβ8. Mouse TGF-β converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. However, human TGF-β1 seems not to take part in T reg differentiation. Murine Th17 response can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORγt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23. Recombinant Human TGF-β1 Applications: Bioassay Optical Density (490 nm) Recombinant Human TGF-β1 (Ala279-Ser390, Accession # NM_000660) was expressed in CHO cells. Mature TGF-β1 was purified from the conditioned media. Bioactive Protein: TGF-β1 inhibits the proliferation of mouse HT-2 cells induced by IL-4. The ED50 is 0.05 to 0.2 ng/mL, corresponding to a specific activity of 0.5 to 2.0 × 107 units/mg. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL TGFβ1 inhibits the proliferation of TH-2 cells induced by IL-4 Bioactive Protein (Carrier-Free) 580702 580704 580706 580708 25 μg 100 μg 500 μg 10 μg $385 $285 $1600 $5000 Please request a quote for amounts greater than 500 µg. Human TNF-α Other Names: TNFSF2, TNF, MCF, Cachectin, Necrosin, Macrophage cytotoxic factor, DIF TNF-α is secreted by macrophages, monocytes, neutrophils, Tcells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Recombinant Human TNF-α Applications: Bioassay Recombinant Human TNF-α (Val 77–Leu 233, Accession # NM_000594) was expressed in E. coli and purified to greater than 98% purity. Optical Density (490 nm) system is involved in the establishment of organ metastasis in different cancers, for example in lymph node metastasis in breast cancer and oral squamous cell carcinoma (SCC), and peritoneal metastasis in ovarian cancer. Recently, several studies have demonstrated the existence of a small subset of cancer cells which share many characteristics with stem cells and named cancer stem cells (CSC). They constitute a reservoir of self-sustaining cells with the ability to maintain the tumor growth. Most of them express CXCR4 receptor and respond to a chemotactic gradient of its specific ligand SDF-1, suggesting that CSC probably represent a subpopulation capable of initiating metastasis. Bioactive Protein: This TNF-α protein is biologically active Cytotoxic effect of human with an ED50 of 0.020 to 0.050 TNFa in L929 cells ng/mL, corresponding to a specific activity of 2 to 5 × 107 units/mg, as determined by dose- dependent stimulation of L929 cells treated with actinomycin D. Endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bottled at 200 µg/mL for sizes 10–100 µg and at higher concentrations for sizes of 500 µg and above. ng/mL Bioactive Protein (Carrier-Free) 570102 10 μg $125 570104 50 μg $200 570106 100 μg $360 570108 500 μg $800 Please request a quote for amounts greater than 500 µg. 452 Toll Free 1.877.BIOLEGEND (246.5343) Human TNF-β Human VEGF-165 Other Names: LT-α, Coley’s toxin, Hemorrhagic factor, Necrosin, NKCF, DIF, TNFSF-1 Other Name: Vascular permeability factor (VPF) Tumor necrosis factor-beta (TNF-β), also known as lymphotoxin-α (LT-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. TNF-β possesses a signal peptide sequence and is a secreted protein; however, TNF-β is also present on the surface of activated T, B and LAK cells as a complex with LT-beta. Bioactive TNF-β exists as a homotrimer. Recombinant Human TNF-β Recombinant human TNF-β is an 18.6 kD protein containing 171 amino acids. Bioactive Protein: This TNF-β protein is bioactive and can be used for in vitro assays. Bioactive Protein 562603 5 μg $125 Human TWEAK (CD255) TWEAK (TNFSF12) is a “TNF-like weak inducer” of apoptosis through a non-death domain-dependent mechanism. TWEAK is a type II membrane protein which exhibits a single internal hydrophobic domain of 27 amino acids in the N-terminal region. TWEAK is proteolytically cleaved to produce a soluble cytokine that signals as a trimerized molecule. Fibroblast growth factor-inducible 14 (Fn14)/TWEAKR has been described as a receptor for TWEAK, and it is associated with proliferation of endothelial cells and angiogenesis. However, TWEAK mediates signal transduction and linear differentiation of monocyte/macrophage cells lacking Fn14/ TWEAKR, suggesting that such cells contain an alternative TWEAK receptor. Elevated levels of TWEAK and/or Fn14 have been found to be associated with the pathogenesis of rheumatoid arthritis, skeletal muscle wasting, systemic lupus erythematosus, multiple sclerosis, stroke, neuroinflammation and neurodegeneration, and several types of cancer. The pathological functions of TWEAK are primarily attributed to its ability to induce the expression of several proinflammatory cytokines, chemokines, cell adhesion molecules, and matrix-degrading enzymes mainly through the activation of NF-B, a major proinflammatory transcription factor. More recently, it has been described that CD163 (a scavenger receptor) might act as a receptor “decoy” for the ligand TWEAK. Recombinant Human VEGF-165 Applications: Bioassay Recombinant human VEGF-165, amino acids Ala27-Arg191 (Accession# NM_001171626) was expressed in E. coli. This protein was purified to greater than 95% purity. Bioactive Protein: This VEGF-165 protein is biologically active with an ED50 = 2.5 to 4.5 ng/mL, corresponding to a specific activity of 2.2–4 × 105 units/mg, as determined by HUVEC cell proliferation in a dose dependent manner. The protein is provided lyophilized, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein 584501 584502 2 μg 100 μg $75 $385 Bioassay-Validated Recombinant Cytokines Applications: ELISA, Bioassay VEGF-165 plays a critical role in new blood vessel formation in vivo. VEGF-165 induces angiogenesis on different levels: it acts as mitogen especially on endothelial cells, raises the vessel permeability and dilation by releasing NO, and has chemotactic impact on monocytes/macrophages, which plays a crucial role in inducing inflammatory neovascularization. Angiogenic activities of VEGFs are mediated primarily through two receptors, VEGFR-1 and VEGFR-2. VEGFR-2 signaling is enhanced by interactions with co-receptors such as heparin/heparan sulfate and Neuropilin 1 (NRP-1). The expression of VEGF-165 in the tumor microenvironment influences the differentiation of bone marrow-derived pericytes to the Ewing’s sarcoma vasculature. VEGF induces osteoclast migration and activation. In addition, it supports the survival of mature osteoclasts. These processes play a key role in repairing and remodeling bone during bone development. VEGF is upregulated by hypoxia and by overexpression of oncogenes such as mutant ras and c-myc in tumours. Immobilized forms of perlecan and PlnDI (inmovilized and soluble) bind VEGF-165 to coordinate developmental angiogenesis modulating VEGF-165/VEGFR-2 signaling. In addition, CXCL8/IL-8 stimulates VEGF expression and VEGFR2 activation in endothelial cells. Recombinant Human TWEAK Applications: Bioassay Recombinant Human TWEAK (Lis97-His249, Accession #NM_003809.2) was expressed in E. coli and purified to greater than 98% purity. Bioactive Protein: This TWEAK protein is biologically active with an ED50 < 10 ng/mL, corresponding to a specific activity of > 1 × 107 units/mg, as determined by the dose dependent stimulation of production of IL-8 by human PBMC. Bottled lyophilized, and endotoxin is less than 0.1 EU/µg (<0.01 ng/µg). Bioactive Protein (Carrier-Free) 566402 5 μg $125 biolegend.com • [email protected] 453