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DataSheet Flt3LigandMouseRecombinant CatalogueNumber IY-340 Fms-relatedtyrosinekinase3ligand,FLK2,STK1,CD135,StemCell Synonyms TyrosineKinase1,FLT3LG,Flt3. FLT3ligandisareceptorfortheflcytokinehasatyrosine-proteinkinase activity&agrowthfactorthatregulatesproliferationofearly Introduction hematopoieticcells.Flt3-LigandsynergizeswithotherCSFsand interleukinstoinducegrowthanddifferentiation. Thesaleand/orcommercialuseofRecombinantAdiponectinisprohibited PatentRights intheUnitedStatesofAmerica(U.S.A). Flt3-LigandMouseRecombinantproducedinE.Coliisnon-glycosylated, polypeptidechaincontaining163aminoacidsandhavingamolecular Description massof18587Dalton.Flt3-Ligandispurifiedbyproprietary chromatographictechniques. Source EscherichiaColi. PhysicalAppearence SterileFilteredWhitelyophilized(freeze-dried)powder. Formulation TheFLT3LMouseproteinwaslyophilizedwithoutanyadditives. ItisrecommendedtoreconstitutethelyophilizedFlt3-Ligandinsterile Solubility 18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdiluted tootheraqueoussolutions. LyophilizedFlt3-Ligandalthoughstableatroomtemperaturefor3weeks, shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFlt-3Ligand shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C. Stability Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1% HSAorBSA). Pleasepreventfreeze-thawcycles. Purity Greaterthan98.0%asdeterminedbySDS-PAGE. MTPDCYFSHSPISSNFKVKFRELTDHLLKDYPVTVAVNLQDEKHCKALWS LFLAQRWIEQLKTVAGSKMQTLLEDVNTEIHFVTSCTFQPLPECLRFVQT Aminoacidsequence NISHLLKDTCTQLLALKPCIGKACQNFSRCLEVQCQPDSSTLLPPRSPIA LEATELPEPRPRQ. TheED50,calculatedbythedose-dependentproliferationofmouseAML5 BiologicalActivity cellsis1.3-2.0ng/ml. ProductsarefurnishedforLABORATORYRESEARCHUSEONLY.The Usage productmaynotbeusedasdrugs,agriculturalorpesticidalproducts,food additivesorhouseholdchemicals.