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Princeton Business Park, 5 Crescent Ave., P O Box 275 Rocky Hill, NJ 08553 USA Tele: 609.497.0253 Fax: 609.497.0321 www.peprotech.com Email: [email protected] Product Information Sheet Recombinant Human GM-CSF Catalog# 300-03 Source: E.coli Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Synonyms: Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-α Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage/Stability: Product Form Temperature Storage Time Lyophilized -20°C to -80°C See expiration date Lyophilized 4°C 12 months Lyophilized RT 1 month Diluted as per -20°C to -80°C 12 months CoA Diluted as per 2°C to 8°C 1 week CoA **Avoid repeated freeze-thaw cycles. Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. For a list of references please visit our website at www.peprotech.com Country of Origin: USA Lot-specific information is not available on Product Information Sheet. See Certificate of Analysis for details. Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures.