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PRODUCT DATA SHEET Recombinant Human GM-CSF Catalog Number: 90025 Description: GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. The GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5qsyndrome and acute myelogenous leukemia. PRODUCT SPECIFICATIONS Product Details: Purified, full-length human recombinant GM-CSF protein (amino acids 18-144, 127 a.a.) produced in human cells. (Accession NP_000749.2; UniProt P04141) Source: Human cell line Predicted Molecular Weight: 14.5 kDa Tag: StrepII at N Cell Culture Medium: Chemically-defined, serum- and animal-product-free culture medium Purity: >95% pure by SDS-PAGE Endotoxin: <0.1EU per g protein by LAL method Biological Activity: ED50 is 0.1-0.25 ng/ml as determined by a TF-1 cell proliferation assay Formulation: Lyophilized from 0.2µm filtered solution in 0.3X PBS with 10% Trehalose (carrier-free). Reconstitution: Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1 mg/ml. Stability & Storage: 12 months at -20°C as supplied; 1 month at 4°C after reconstitution. Avoid freeze/thaw cycles. Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETS CATQIITFESFKENLKDFLLVIPFDCWEPVQE SDS-PAGE of 2 g purified recombinant GM-CSF. The bioactivity of the purified recombinant GM-CSF was determined by a TF-1 cell proliferation assay. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. PlexBio Co., Ltd. 6F-1 No. 351 Yangguang St., Neihu District, Taipei 11491 Version 1.1 315130 tel. | +886-2-2627-5878 email | [email protected] web | www.plexbio.com