* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download CIT - Cork Institute of Technology
Promoter (genetics) wikipedia , lookup
Epitranscriptome wikipedia , lookup
Eukaryotic transcription wikipedia , lookup
Genetic engineering wikipedia , lookup
Biochemistry wikipedia , lookup
DNA supercoil wikipedia , lookup
Molecular cloning wikipedia , lookup
Non-coding DNA wikipedia , lookup
Gene expression wikipedia , lookup
Transformation (genetics) wikipedia , lookup
Community fingerprinting wikipedia , lookup
Transcriptional regulation wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Silencer (genetics) wikipedia , lookup
Genetic code wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Biosynthesis wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Real-time polymerase chain reaction wikipedia , lookup
Molecular evolution wikipedia , lookup
CORK INSTITUTE OF TECHNOLOGY INSTITIÚID TEICNEOLAÍOCHTA CHORCAÍ Winter Examinations 2012/13 Module Title: Introductory Molecular Biology Module Code: GENE7001 School: Science Programme Title(s): Bachelor of Science in Biomedical Science Programmes Code(s): SBISC_8_Y2 External Examiner(s): Prof Richard O’Kennedy Internal Examiner(s): Mr Christopher Johnston Instructions: Answer 4 out of 5 questions. All questions carry equal marks. Duration: 2 hours. Sitting: Winter. Semester 1 2012 Requirements for this examination: Note to Candidates: Please check the Programme Title and the Module Title to ensure that you have received the correct examination. If in doubt please contact an Invigilator. Page 1 of 3 Q1. a) Describe the experiments of Fred Griffith and the Hershey/Chase experiment. How did they contribute to determining DNA was the genetic material? (15marks) b) Describe the three associated enzymatic activities of E.coli DNA polymerase. (5marks) c) Draw the structure of the 4 nitrogenous bases associated with DNA, indicate which are purines and which are pyrimidines (5marks) (25 marks) Q2. a) What are the ingredients needed to conduct the Polymerase Chain Reaction (PCR) on a gene and what are the function/s of each component. (10marks) b) In relation to chromosome abnormalities, describe the different types of structural abnormalities. (10marks) c) Explain what is meant by the term eukaryote RNA splicing. (5marks) (25 marks) Q3. In relation to bacterial conjugation (using diagrams where appropriate): a) Describe the different types of E. coli donor and recipient strains. (10 marks) b) Describe a typical conjugative mating event between two of the strains you have described. (15 marks) (25 marks) Page 2 of 3 Q4. Write a detailed account of the manner in which a mRNA template is translated to a polypeptide chain in a prokaryotic cell. (25 marks) Q5. a) Discuss the structure of the trp operon and its regulation in both the presence and absence of tryptophan. (15 marks) b) In relation to point mutations within a coding region of a gene, differentiate between the following mutations and indicate their effect at an amino acid level. i. ii. iii. iv. Silent Missense Nonsense Frameshift (10 marks) (25 marks) Page 3 of 3