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La ng a Fa ra l C l ar 2 o ch 01 lle iv 1 - ge ed BIOLOGY 2370: MICROBIOLOGY I COURSE OUTLINE FALL 2011 Instructors Textbooks Required Optional Lecture: Dr. Eileen Rawling Office: A303m Phone: 604-323-5517 email: [email protected] Laboratory: Anita or Jason Office: A384 Phone: 604-323-5338 [email protected] [email protected] 1. Bergman, A., Langara College, 2011. Microbiology Laboratory Exercises 1. Prescott, L., et al. 2011. Microbiology 8th ed. (older editions are fine) 2. Leboffe et al. any edition A Photographic Atlas for the Microbiology Laboratory Learning objectives 1. To learn the basic language of microbiology. 2. To understand the relationship between the structure and function of bacterial macromolecules. 3. To learn how bacteria differ from other organisms. 4. To learn to synthesize information and apply it to new situations.. 5. To enjoy and appreciate the microbial world! Requirements 1. Students are required to attend all laboratories. There are no make-up laboratories. 2. A letter from a medical doctor is required if you miss a laboratory or examination. 3. There are no make up exams. Final exams must be written during the designated time. 4. To pass the course, the student must receive at least 50% in the lab. 5. Plagiarism, in any form, is unacceptable and will result in a penalty. Grades (%) A+ A AB+ B B- 90-100 85-89 80-84 77-79 73-76 70-72 C+ C CD F 65-69 60-64 55-59 50-54 0-49 Lecture Midterm I Lecture Midterm II Lecture Final Lab Midterm I Lab Final Lab Exercises 20% 25% 25% 12.5% 12.5% 5% 100% handout Appendix I Introduction to microscopy A. Cell/virus size B. Definitions C. Electromagnetic radiation D. Oil immersion E. Limit of resolution F. Microscopes and sample preparation handout Chapter 2 Prokaryotic cell structure and function A. Morphology B. Cell Overview C. Inside the cell D. Bacterial plasma membrane E. Bacterial cell wall overview F. Gram-positive cell wall G. Gram-negative cell wall H. Peptidoglycan synthesis I. Extracellular substances J. Appendages: Pili and Flagella K. Chemotaxis L. Endospores M. Archaea Chapter 3 Microbial Nutrition andGrowth A. Nutrients B. Culture media C. Isolation of a pure culture D. Replication E. Growth in a batch culture F. Continuous culture G. Environmental factors affecting growth H. Growth in a natural environments Chapter 6,7 (5,6) Control of microbial growth A. Overview B. Physical methods of control C. Chemical methods of control D. Selection of antimicrobial procedure E. D and Z values Chapter 8 (7) Metabolism A. Definitions/concepts B. Introduction to metabolism C. Chemoheterotrophs D. Three examples of chemoheterotrophs: E. coli , Pseudomonas, lactic acid bacteria. Chapters 9, 10,11 (8, 9, 10) La ng a Fa ra l C l ar 2 o ch 01 lle iv 1 - ge ed Introduction to microbiology A. Review of cell chemistry B. Conventions p 143-146 (105-110) (also p. 575) p. 271-273 (232-235) p. 346-349 (184-187) Chapter 18 (20) La ng a Fa ra l C l ar 2 o ch 01 lle iv 1 - ge ed E. Autotrophs F. Chemolithotrophs G. Phototrophs H. Anabolism I. Applied microbial metabolism Nucleic acid and protein synthesis A. Definitions B. DNA structure and replication C. RNA structure and synthesis D. Protein synthesis Chapter 12 (11) Regulation of Gene Expression A. lac operon B. Two-component regulatory systems C. Regulation of transcription elongation D. Regulation of translation C. Global regulatory systems Chapter 13 (12) Microbial Genetics A. Mutations B. Plasmids and transposons B. Recombination C. Horizontal gene transfer D. Conjugation E. Transformation F. Transduction G. Barriers to DNA transfer Chapter 14 (13) Microbiology 2370 Lab Schedule – Fall 2011 Exercises: La ng a Fa ra l C l ar 2 o ch 01 lle iv 1 - ge ed Date: Sept. 8 1: Use of a Brightfield Compound Microscope 2: Microscopic Measurement Sept. 15 3: Aseptic Transfer Techniques 4: Examining Stained Bacteria 5: Observing Cyanobacteria Sept. 22 6: Smear Preparation – Simple Staining 7: Gram Staining 8: Negative Staining Sept. 29 Oct. 6 9: Endospore Staining 10: Oxygen Requirements MIDTERM LAB EXAM (A366) Oct. 13 11: 12: 13: 14: Oct. 20 14: Soil Lab Continued 15: Quantifying Bacteria Oct. 27 16: Temperature 17: Ultraviolet Radiation and Photoreactivity Nov. 3 18: Lysine Decarboxylase 19: Selected Microbial Enzymes 20: Enzymatic Attacks on Nitrogen-containing Substrates Nov. 10 Ubiquity of Microorganisms and Culture Characteristics Pour-Plate Techniques Streak & Spread-Plate Techniques Isolation of Antibiotic Producing Bacteria from Soil FINAL LAB EXAM (A365)