Download Fall 2011 - Langara College

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BIOLOGY 2370: MICROBIOLOGY I
COURSE OUTLINE FALL 2011
Instructors
Textbooks
Required
Optional
Lecture: Dr. Eileen Rawling
Office: A303m
Phone: 604-323-5517
email: [email protected]
Laboratory: Anita or Jason
Office: A384
Phone: 604-323-5338
[email protected]
[email protected]
1. Bergman, A., Langara College, 2011. Microbiology Laboratory Exercises
1. Prescott, L., et al. 2011. Microbiology 8th ed. (older editions are fine)
2. Leboffe et al. any edition A Photographic Atlas for the Microbiology Laboratory
Learning
objectives
1. To learn the basic language of microbiology.
2. To understand the relationship between the structure and function of bacterial
macromolecules.
3. To learn how bacteria differ from other organisms.
4. To learn to synthesize information and apply it to new situations..
5. To enjoy and appreciate the microbial world!
Requirements
1. Students are required to attend all laboratories. There are no make-up
laboratories.
2. A letter from a medical doctor is required if you miss a laboratory or
examination.
3. There are no make up exams. Final exams must be written during the
designated time.
4. To pass the course, the student must receive at least 50% in the lab.
5. Plagiarism, in any form, is unacceptable and will result in a penalty.
Grades (%)
A+
A
AB+
B
B-
90-100
85-89
80-84
77-79
73-76
70-72
C+
C
CD
F
65-69
60-64
55-59
50-54
0-49
Lecture Midterm I
Lecture Midterm II
Lecture Final
Lab Midterm I
Lab Final
Lab Exercises
20%
25%
25%
12.5%
12.5%
5%
100%
handout
Appendix I
Introduction to microscopy
A. Cell/virus size
B. Definitions
C. Electromagnetic radiation
D. Oil immersion
E. Limit of resolution
F. Microscopes and sample preparation
handout
Chapter 2
Prokaryotic cell structure and function
A. Morphology
B. Cell Overview
C. Inside the cell
D. Bacterial plasma membrane
E. Bacterial cell wall overview
F. Gram-positive cell wall
G. Gram-negative cell wall
H. Peptidoglycan synthesis
I. Extracellular substances
J. Appendages: Pili and Flagella
K. Chemotaxis
L. Endospores
M. Archaea
Chapter 3
Microbial Nutrition andGrowth
A. Nutrients
B. Culture media
C. Isolation of a pure culture
D. Replication
E. Growth in a batch culture
F. Continuous culture
G. Environmental factors affecting growth
H. Growth in a natural environments
Chapter 6,7 (5,6)
Control of microbial growth
A. Overview
B. Physical methods of control
C. Chemical methods of control
D. Selection of antimicrobial procedure
E. D and Z values
Chapter 8 (7)
Metabolism
A. Definitions/concepts
B. Introduction to metabolism
C. Chemoheterotrophs
D. Three examples of chemoheterotrophs:
E. coli , Pseudomonas, lactic acid bacteria.
Chapters 9, 10,11
(8, 9, 10)
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Introduction to microbiology
A. Review of cell chemistry
B. Conventions
p 143-146 (105-110)
(also p. 575)
p. 271-273 (232-235)
p. 346-349 (184-187)
Chapter 18 (20)
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E. Autotrophs
F. Chemolithotrophs
G. Phototrophs
H. Anabolism
I. Applied microbial metabolism
Nucleic acid and protein synthesis
A. Definitions
B. DNA structure and replication
C. RNA structure and synthesis
D. Protein synthesis
Chapter 12 (11)
Regulation of Gene Expression
A. lac operon
B. Two-component regulatory systems
C. Regulation of transcription elongation
D. Regulation of translation
C. Global regulatory systems
Chapter 13 (12)
Microbial Genetics
A. Mutations
B. Plasmids and transposons
B. Recombination
C. Horizontal gene transfer
D. Conjugation
E. Transformation
F. Transduction
G. Barriers to DNA transfer
Chapter 14 (13)
Microbiology 2370 Lab Schedule – Fall 2011
Exercises:
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Date:
Sept. 8
1: Use of a Brightfield Compound Microscope
2: Microscopic Measurement
Sept. 15
3: Aseptic Transfer Techniques
4: Examining Stained Bacteria
5: Observing Cyanobacteria
Sept. 22
6: Smear Preparation – Simple Staining
7: Gram Staining
8: Negative Staining
Sept. 29
Oct. 6
9: Endospore Staining
10: Oxygen Requirements
MIDTERM LAB EXAM (A366)
Oct. 13
11:
12:
13:
14:
Oct. 20
14: Soil Lab Continued
15: Quantifying Bacteria
Oct. 27
16: Temperature
17: Ultraviolet Radiation and Photoreactivity
Nov. 3
18: Lysine Decarboxylase
19: Selected Microbial Enzymes
20: Enzymatic Attacks on Nitrogen-containing Substrates
Nov. 10
Ubiquity of Microorganisms and Culture Characteristics
Pour-Plate Techniques
Streak & Spread-Plate Techniques
Isolation of Antibiotic Producing Bacteria from Soil
FINAL LAB EXAM (A365)