Download Enhancing the Innate Immune System with

Enhancing the Innate Immune
System with Brolico®
Introduction to Brolico® and its Health Benefits
During a collaborative research project conducted by Imagine Global Care
Co., Ltd., University of Tokyo and Genome Pharmaceuticals Institute,
research scientists discovered a new phytonutrient found in and extracted
from the vegetable broccoli. The new phytonutrient is called brolico.
Brolico cannot be absorbed by the body by eating broccoli, since it is
expelled by the body without being absorbed. Because of this limitation,
scientists developed a patented extraction method which resulted in a
supplemental form of brolico called Brolico® which can be absorbed by
the body. The Brolico® extraction method is patented under US Patent
number 8,313,779, which was recorded in November 2012.
In various studies of the Brolico® supplement, researchers discovered that
the brolico nutrient is able to produce high levels of cellular activity within
crucial white blood cells, in effect, raising innate or natural immunity. In
fact, the Brolico® supplement produced record-breaking levels of
activation among crucial white blood cells such as:
natural killer cells (NK cells)
neutrophils
The effects of Brolico® are very promising on immune system function:
Increases NK cell activity approximately 60 times more than
fucoidan*
Helps and supports the defense mechanism of your immune
system*
Increases natural killer cell activity*
Increase and elevates neutrophils*
Decreases the pro-inflammatory cytokine Interleukin 6
(IL-6)*
Promoted elimination of infected or harmful cells from the
body*
The Human Immune System and Innate Immune System
The human immune system is a defense system that is composed of many
biological structures and processes with the purpose of protecting against
disease. The immune system is classified into two sub-systems:
Innate immune system
Adaptive immune system
Source: StudyBlue
The innate immune system, also called the natural or native immune
system, is based on the genetic constitution of the person, without any
external stimulation or previous infection. It is considered the first-line of
defense of the body’s biological system from various pathogens. The
innate immune system does not provide long-lasting immunity to the host,
but provides immediate defense against infection.
Innate immunity is divided into two types:
Non-Specific innate immunity: A degree of natural resistance to
all infections in general
Specific innate immunity: This is a natural resistance to a
particular kind of germ only.
The major functions of the innate immune system include:
Recruiting immune cells to sites of infection, through the
production of chemical factors, including specialized
chemical mediators, called cytokines
Activation of the complement cascade to identify bacteria,
activate cells, and promote clearance of antibody
complexes or dead cells
Identification and removal of foreign substances present in
organs, tissues, blood and lymph, by specialized white
blood cells
Activation of the adaptive immune system through a
process known as antigen presentation
Acting as a physical and chemical barrier to infectious
agents
The adaptive immune system, is composed of highly specialized, systemic
cells and processes that eliminate pathogens or prevent their growth.
Adaptive immunity creates immunological memory after an initial
response to a specific pathogen, and leads to an enhanced response to
subsequent encounters with that pathogen.
Components of the immune system
Innate immune system
Adaptive immune system
Response is non-specific
Pathogen and antigen specific response
Exposure leads to immediate maximal response
Lag time between exposure and maximal response
Cell mediated &
humoral components
Cell mediated and
humoral components
No immunological memory
Exposure leads to immunological memory
Found in nearly all forms of life
Found only in jawed vertebrates
Source: Wikipedia: Immune System
Two very important components of the innate immune system are
neutrophil and natural killer cells (NK cells).
Neutrophil are the most abundant type of granulocytes and the most
abundant (40% to 75%) type of white blood cells. During the beginning
(acute) phase of inflammation, neutrophils are one of the first-responders
of inflammatory cells to migrate towards the site of inflammation.
Natural killer cells or NK cells are a type of cytotoxic lymphocyte created in
the bone marrow of the lymphatic system and provide rapid responses to
viral-infected cells, acting at around 3 days after infection, and respond to
tumor formation. Natural killer (NK) cells, known to play a major role in
the innate immune system, constantly patrol the body and destroy cancer
cells and virus-infected cells.
Increasing neutrophil and NK cell activity is important to enhancing the
innate immune system and defending the body by pathogens. In fact, NK
cells are the body’s first line of defense against the daily onslaught of
pathogens. Therefore optimizing NK cells provides greater protection and
defense against foreign invaders.
T-cells and macrophages secrete Interleukin 6 (IL-6) to stimulate an
immune response. IL-6 acts as both a pro-inflammatory cytokine and an
anti-inflammatory myokine. IL-6 stimulates the inflammatory and autoimmune processes in many diseases. An elevated and excessive secretion
of IL-6 may increase the risk of many diseases, especially atherosclerosis.
Studies on the Effectiveness of Brolico® Enhancing the Innate
Immune System
A 2011 study entitled Comparison Study to Determine Immunity Activation
Effectiveness Produced by Functional Food Extracts with Proposed
Immunity Stimulation Effects, revealed a comparison study of various well
known natural immunity activating extracts using a new active assay
(silkworm muscle contraction), which was originally developed by faculty
at Tokyo University Department of Pharmaceutical Sciences.
Scientists discovered that when injecting a silkworm with a component
that activates natural immunity, such as a bacteria or fungus, the muscles
of the silkworm gradually shrink. This is due to the fact that when the
activated component binds with the immune cell of the silkworm, active
oxygen is released, and a specific protein is activated, which causes the
muscle to contract. This muscle contraction can be measured by the
degree of contraction in order to determine the precise level of specific
activity (immunity activation) produced by the given component.
The researchers of this study used 10 extracts that are attributed to effect
immunity stimulation for comparison against Brolico®. These 10 extracts
were:
açaí (berry fruit from Euterpe oleracea)
bee propolis
beta-glucan (from cell walls of cereals, yeast and bacteria)
broccoli sprouts
DHA (omega-3 fatty acid)
EPA (omega-3 fatty acid)
fucoidan (fund in brown algae)
lentinan (from Shiitake mushroom)
sulforaphane (from broccoli)
The results of the test were quite astonishing and showed that the
phytonutrient extract of brolico (Brolico®) had the highest level of specific
activity measuring approximately 770 units per milligram. The other 10
extracts measured far below Brolico® with lentinan measuring the second
highest with 290 units per milligram.
The Table below shows the measurements of each extract’s performance
(units of specific activity per milligram), as well as the comparison
evaluation results for each and Brolico®:
Comparison of Brolico® and 10 Extracts
Extracts
Specific
Activity
(units/mg)
Brolico®
770
Lentinan (from Shiitake mushroom)
290
Approx 2.6x more
Broccoli sprouts (crude extracts)
18
40x more
Performance
Comnparison
Extracts
Specific
Activity
(units/mg)
Performance
Comnparison
Beta-glucan (from cell walls of
cereals, yeast and bacteria)
14
Approx 50x more
Fucoidan (fund in brown algae)
12
Approx 60x more
DHA (omega-3 fatty acid)
10
70x more
Sulforaphane (from broccoli)
7
100x more
EPA (omega-3 fatty acid)
4
190x more
Açaí (berry fruit from Euterpe
oleracea)
3.1
Approx 240x more
0.40
1000x more
Bee propolis (ethanol extracts,
crude extracts)
Source
The researchers concluded that:
“the results suggest brolico to be capable of producing extraordinarily
high levels of natural immunity stimulation, much higher those produced
by its counterparts. Thus, there appears a significant benefit to further
research on a much broader scale, specifically in regards to human
trials.” 1
Source: brolico.com
A research study published in 2012 in the Journal Japanese Pharmacology
& Therapeutics (Vol.40 no.6 2012) entitled Exploratory Trial Concerning
Activation of Human Natural Immunity by Continued Consumption of a
Broccoli Extract Processed Food Product investigated the activating effect
on human immune cells and safety of continuously consumed Brolico®.
Researchers at the University of Tokyo Graduate School of Pharmaceutical
Sciences have found that muscles of silkworm larvae contract by the
action of the innate immune system. Researchers at Imagine Global Care
Co., Ltd. in collaboration with the University of Tokyo Graduate School of
Pharmaceutical Sciences sought out immunostimulatory substances using
muscle contraction of silkworms as an indicator. What they found was
that Brolico® was found to have the potential for displaying an
immunostimulatory effect that may exceed that of beta-glucan.
Test subjects were tested for three endpoints before and after consuming
Brolico®. These three endpoints included:
Neutrophil phagocytic capacity
NK cell activity
IL-6 levels
Changes in natural immunity-mediating cells NK cell activity and
neutrophil phagocytic capacity tended to increase after consumption of
the test food (p < 0.1 for both) (See Table 1). Levels of the proinflammatory cytokine, IL-6 actually decrease after consumption. No
significant changes in the hemogram levels were noted between the
values before and after consumption.
Source: Exploratory Trial Concerning Activation of Human Natural Immunity by Continued
Consumption of a Broccoli Extract Processed Food Product
The researchers concluded their study with the promising statement:
“The observed trend toward increased NK cell activity and neutrophil
phagocytic capacity after consumption of the test food suggests that
brolico contributes to activation of cells involved in natural immunity.”
2
This study from 2012 suggests that the extracted broccoli constituent
enhances innate immunity.
Informational References:
Brolico Research
Resources:
Brolico® can be purchased at brolico.com
*The statements on this page have not been evaluated by the FDA. Products mentioned on this page are not intended to
diagnose, treat, cure or prevent any disease. The information on this page is for educational purposes only, and does not
constitute medical advice. For medical advice please contact a licensed healthcare professional.