Download Exam 1 Public v2 Bio200 Win16

Biology 200, Winter 2016
Exam 1
Name: ________________________________________
TA: __________________
Biology 200
Winter Quarter 2016
Exam #1
DO NOT OPEN EXAM UNTIL DIRECTED TO DO SO
• Make sure you have 4 pages of questions and six pages total.. Print your
name and information on all pages.
• Please use a pen. Pen is much easier to read, even with extensive
crossing-out. Pencil-written exams are acceptable, but may not receive full
credit to penciled answers on regrades.
• When asked, provide concise and clearly written answers. We may deduct
points if you do not fully answer the question or if your answer is too
vague or too confusing for us to follow.
• Extra information, if incorrect, will lose points.
• Limit your answers to the space provided. If you need extra space, you
can use the bottom of this first page. Indicate “on first page” where necessary.
Page
Points Awarded
2 ______ out of 20 points
3 ______ out of 22 points
4 ______ out of 18 points
5 ______ out of 20 points
Total ______ out of 80 points
Diagram of Proteins G and H:
Please note that only a small portion of the total biochemical features are shown. Most of the diagram
shows Protein G, and the entirety of Protein H is simplified in the geometric cartoon.
Protein G
Exam 1
Name:_____________________________
[20 points] Proteins and Organelles
Protein diagram 1 shows some features of the structure of Protein G. For clarity, few of the R-groups
are shown (and some parts are not to scale). Use this diagram to answer Q1a-c.
/5 1a) The shape of Protein G has evolved to fit precisely as part of a biochemical pathway.
Mark all correct answers.
_____ Changing the primary structure of Protein G is likely to change Protein G function.
_____ Changing the secondary structure of Protein G is likely to change protein Protein G function.
_____ Changing the tertiary structure of Protein G is likely to change protein Protein G function.
_____ Changing the quaternary structure of Protein G is likely to change protein Protein G function.
Explain the overall trend in your answers in 2-3 sentences, maximum.
/5
1b) Proteins G and H bind tightly. Which of these amino acids is most likely to be part of the
[location withheld]. Circle the structure of the single best answer.
[There will be 4-5 amino acid structures shown here.]
/5 1c) Protein G is extremely stable when located normally within the smooth endoplasmic
reticulum, but
[additional
information
withheld].
Why?
Explain your answer in 2-3 sentences, maximum.
/5 2) Two unicellular species are nearly identical, except for one thing: Cell Y requires an organellelike compartment for completion of a specific task, while Cell Z completes the same task without the
organelle-like compartment.. It appears that the task is similar to that performed in your [name of
organelle withheld]. In 3 sentences, maximum, propose two specific advantages that Cell Y may have over Cell
Z and also [proposed feature withheld].
Total: _____
Page 2 of 6
Exam 1
Name:_____________________________
[22 points] Enzymes on the cellular surface
Concentration Concentration
/5 3a) The following data shows the intra- and interoutside of cell inside of cell
cellular concentrations of three molecules. To survive,
[data withheld] [data withheld]
Ethane
this cell needs to [transport priority withheld]. Through
evolution, these cells have found the best possible
[data withheld] [data withheld]
Lactose
method for this transport.
[data withheld] [data withheld]
Estrogen
Mark all correct answers.
_____ The cell is likely to use facilitated diffusion.
_____ The cell is likely to use passive diffusion.
_____ The cell is likely to use active transport.
_____ This method of transport will require a transmembrane protein.
/5
3b) Which molecule is most likely to pass through a lipid bilayer at the [highest/lowest] rate?
Circle the structure of the single best answer.
[There will be 4-5 molecular structures shown here.]
/5
3c) You discover that this particular enzyme is unusual because, unlike a normal enzyme, it
[withheld]. Why would this tend to make this enzyme less helpful to the organism? Explain your idea in
2 sentences, maximum.
/7
3d) To compete more effectively, a few of these cells have become murderous; they actively
attempt to kill other cells. They do this by inserting a poison molecule through a mechanism that
requires [withheld detail about either targeted cell crawling or sending of poison-filled vesicles].
Mark all correct answers.
_____ This process will require the coordinated function of many types of proteins.
_____ This process will require the function of many copies of the same type of protein.
_____ This process will move cytoplasm towards the target cell.
_____ This process will require the successful bending of plasma membranes.
Draw a simplified diagram of this process here.
Total: _____
Page 3 of 6
Exam 1
Name:_____________________________
[18 points] A lonely ribozyme
A ribozyme is a nucleic acid that is capable of folding into a shape and catalyzing a particular
reaction. Imagine a ribozyme inside of a vesicle inside of the nucleus inside of a Blepharisma cell.
/7 4a) Which of the following are visible in a light microscope like the one that you used in the
Bio200 lab? Mark all components as ‘V’ for Visible, ‘I’ for Invisible, and ‘?’ for Unsure.
_____ A base pair in the ribozyme
_____ The nucleus
_____ The blepharisma cell
______ [withheld]
______ [withheld]
______ [withheld]
______ [withheld]
______ [withheld]
______ [withheld]
/2 4b) On a different microscope, you are having problems seeing [detail withheld]. What is one
thing you could try to do differently in your operation of the microscope that might help? Explain in 1
sentence, maximum. Be sure to include why this change might help.
/5 4c) The ribozyme has [structural feature withheld]. Compare this to the structural features in a
protein. Mark the single best answer.
______ This is most similar to primary structure in proteins
______ This is most similar to secondary structure in proteins
______ This is most similar to tertiary structure in proteins
______ This is most similar to quarternary structure in proteins
Explain the similarities in 1-2 sentences, maximum.
/4 4d) [Question about the structure of the ribozyme withheld.]
Explain your idea in 2-3 sentences, maximum.
Total: _____
Page 4 of 6
Exam 1
[20 points] Biomolecules
Name:_____________________________
/14
5a) Fill in all blank boxes in the table shown. If a box is filled with grey, skip it.
Molecular structure
Molecular Type of
Typical biological
formula
Biomolecule role(s)
[structure withheld]
information
storage
Does this contain useful
chemical energy?
If so, put an ‘O’ on the
energy containing part.
If not, put an ‘O’ here.
C6
H12
O6
Put a dashed line around
the part of this molecule
that you would most
expect to find in [location
withheld]?
[structure withheld]
[structure withheld]
lipid
[structure withheld]
C4143
H6209
N1716
O1449
S32
Put a triangle on the part
of the molecule most
likely to contact [molecule
withheld].
[question withheld]
/6
5b) The diagram on the next page shows a complex biochemical reaction happening within a
cell. Using this diagram, select and circle the correct answers to the following four questions.
5bi) Entropy is [INCREASING / DECREASING] in this reaction.
5bii) A hydrogen bond is likely to form among the products of this reaction: [TRUE OR FALSE].
5biii) This reaction includes a [ POLYMERIZATION / DEPOLYMERIZATION / NEITHER ].
5biv) The molecule that is most obviously polar is [ A / B / C / D].
Total: _____
Page 5 of 6
Exam 1
Name:_____________________________
Diagram withheld
Total: _____
Page 6 of 6