Download Clinical immunology The course includes laboratory exercises

Clinical immunology
The course includes laboratory exercises focused on the presentation and individual preparation of
the selected immunological techniques most commonly used for the evaluation of phenotypical and
functional characteristics of innate and adaptive immune systems. The main topics will include:
peripheral blood lymphocyte isolation and cultures, flow cytometry and FACS analysis, monocyte and
lymphocyte subsets isolation using antibody-coated magnetic beads, identification of functional
subsets of T cells by staining for cytokines, stimulation of lymphocyte proliferation by treatment with
polyclonal mitogens or specific bacterial antigen, measurements of apoptosis, ELISA tests for
cytokines identification, phagocytosis evaluation techniques.
Teachers:
1. Lidia Gackowska, PhD
2. Anna Helmin-Basa, PhD
3. Andrzej Eljaszewicz , PhD
4.
Izabela Kubiszewska , MSc
5. Milena Januszewska , MSc
6. Małgorzata Wiese, MSc
Contact: [email protected], [email protected]
Syllabus
I.
Clinical Immunology
II.
Head of the unit: dr hab. Jacek Michałkiewicz, prof. UMK
III.
Faculty of Medicine, Medical Program, 2nd year
IV.
Course coordinator: dr hab. Jacek Michałkiewicz prof. UMK
V.
Form of classes: lectures, tutorials
VI.
Form of crediting: Credit with grade, 5 ECTS points
VII.
Number of hours: 30 (lectures), 30 (tutorials)
VIII.
Aim of the course:
The immunology course for the group of the foreign medical students studying at Faculty of
Medicine second year will involve the basic subjects concerning the structures and functions of the
immune system and their significance in health and disease.
The first part of the course will include lectures on clinical immunology. The main topics will
involve the introduction to immunology, basic features of innate and adaptive immune systems, the
immune system failures in the course of infection, allergy, hypersensitivity, autoimmunity and
transplantation.
The course includes laboratory exercises (starting Feb, 13th 2013) focused on the
presentation and individual preparation of the selected immunological techniques most commonly
used for the evaluation of phenotypical and functional characteristics of innate and adaptive
immune systems. The main topics will include: peripheral blood lymphocyte isolation and cultures,
flow cytometry and FACS analysis, monocyte and lymphocyte subsets isolation using antibody-coated
magnetic beads, identification of functional subsets of T cells by staining for cytokines, stimulation of
lymphocyte proliferation by treatment with polyclonal mitogens or specific bacterial antigen,
measurements of apoptosis, ELISA tests for cytokines identification, phagocytosis evaluation
techniques.
IX. Topics of lectures / tutorials:
The main topics concerning innate immunity – the introduction to the laboratory exercises:

General mechanisms of innate immune system action

Phagocytosis-types of cells involved, effector mechanisms, regulations

Pathogen recognition receptors (Toll and NOD systems)

Complement system main characteristics

Leucocyte rolling, adhesion, leucocyte extravasation

The main topics concerning adaptive immunity- the introduction to the laboratory exercises:

Lymphocyte receptors generation

Mechanisms of antigen recognition by lymphocytes

Antigen presenting cells (professional and non-professional)

Co-stimulation as a second and third signal of T cells induction
Exercise 1. 13-15.02.2013
Topic: Cells of the Immune System – characteristic of cell populations and subpopulations.
Lidia Gackowska, PhD
Exercise 2. 20-22.02.2013
Topic: The analysis of cell phenotype in immunodeficiencies, the flow cytometry method.
Lidia Gackowska, PhD
Exercise 3. 27.02.-01.03.2013
Topic: Antigen Recognition and Dendritic Cells.
Małgorzata Wiese, MSc
Exercise 4. 06-08.03.2013
Topic: Mechanisms of cytotoxicity.
Milena Januszewska, MSc
Exercise 5. 13-15.03.2013
Topic: Components of the innate immune system – the evaluation of the function of phagocytic cells.
Anna Helmin-Basa, PhD
Exercise 6. 20-22.03.2013
Topic: Cell isolation and culture procedures in immunology
Izabela Kubiszewska, MSc
Exercise 7. 27.03-05.04.2013
Topic: Flow cytometric measurement of intracellular proteins
Andrzej Eljaszewicz, PhD
X.
Self-study topics:
----------XI.
Booklist:
Basic literature
1. Kenneth M. Murphy, Paul Travers, Mark Walpor
2. Janeway’s Immunobiology, Garland Science available online at:
3. http://www.ncbi.nlm.nih.gov/books/NBK10757/ (free)
4. http://www.ncbi.nlm.nih.gov/sites/entrez?db=books (free)
5. David Male, Ivan Roitt, Johathan Brostoff Immunology, Mosby.
6. Abbas, Lichtman: Basic Immunology, Sauders.
7. Helen Chapel, Mansel Haeney, Siraj Misbah, Neil Snowden Essentials of Clinical Immunology,
Blackwell.
8. W. Luttmann, K. Bratke, M. Kupper, D. Myrtek Immunology Elsevier Science & Technology
Additional literature:
1. Manjula Reddy, et al; “Comparative analysis of lymphocyte activation marker expression and
cytokine secretion profile in stimulated human peripheral blood mononuclear cell
culture: an in vitro model to monitor cellular immune function” Journal of Immunological
Methods 2004. 293:127-142
2. Marzio R. et al.; „CD69 and regulation of the immune function“; Immunopharmacology and
immunotoxicology 1999. 21:565-582
3. Claman Prussin, Dean D. Metcalfe; “Detection in intracytoplasmic cytokine using flow
cytometry and directly conjugated anti-cytokine antibodies”; Journal of Immunological
Methods 1995. 188:117-128
4. Sewell W.A., et al.;”Determination of intracellular cytokines by flow-cytometry following
whole-blood culture”; Journal of Immunological Methods 1997. 209:67-74
5. Beate Mascher; et al.; “Expression and kinetic of cytokines determinated by intracellular
staining using flow cytometry”; Journal of Immunological Methods 1999. 223:115-121
6. V Hemalatha, P Srikanth, M Mallika; SUPERANTIGENS – CONCEPTS, CLINICAL DISEASE AND
THERAPY; Indian Journal of Medical; (2004) 22 (4):204-211;
7. Ulrich Sack, Atilla Tarnock, Gregor Rothe
8. Cellular Diagnostic - basic principles, methods and applications of flow cytometry, ifcc.
Rules and regulations
1. The clinical immunology course includes lectures (30hours) and tutorials (30 hours)
2. Attendance is obligatory at each lecture and tutorial.
3. Each change between the groups must be approved by the teacher.
4. Students are expected to be punctual.
5. In case of absence students must present a sick leave.
6. Students should have the basic knowledge of the tutorial topics as well as have to actively
participate in the classes in order to accomplish the course.
7. Each tutorial includes a 10-point quiz, which is a form of evaluation of students’ knowledge,
8. Students who did not take the test because of the absence need to pass it individually no
later than two weeks after return from the sick leave.
9. To pass tutorials students need to collect 60% of total points
10. Students who receive less that 60% must take a test from knowledge presented in all
tutorials.
11. The clinical immunology course ends with a credit-grade assigned for lectures and 60% of
tutorials.