Download IMMUNOLOGY The course includes laboratory exercises focused

IMMUNOLOGY
The course includes laboratory exercises focused on the presentation and individual preparation of
the selected immunological techniques most commonly used for the evaluation of phenotypical and
functional characteristics of innate and adaptive immune systems. The main topics will include:
peripheral blood lymphocyte isolation and cultures, flow cytometry and FACS analysis, monocyte and
lymphocyte subsets isolation using antibody-coated magnetic beads, identification of functional
subsets of T cells by staining for cytokines, stimulation of lymphocyte proliferation by treatment with
polyclonal mitogens or specific bacterial antigen, measurements of apoptosis, ELISA tests for
cytokines identification, phagocytosis evaluation techniques.
TEACHERS:
1. dr Lidia Gackowska
2. dr Anna Helmin-Basa
3. dr Andrzej Eljaszewicz
4. mgr Izabela Kubiszewska
5. mgr Milena Januszewska
6. dr Małgorzata Wiese
CONTACT: [email protected], [email protected]
SYLABUS
I.
Name of the unit offering the course: Department of Immunology
II.
Head of the Unit/course coordinator: prof. dr hab. Jacek Michałkiewicz
III.
2nd year, number of hours: 45
IV.
Form of the classes: lecture: 15, tutorials: 15, seminars:15
V.
Form of crediting: credit with grade
VI.
Number of ECTS points: 6
VII.
The main topics
o
Lectures:
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General mechanisms of innate immune system action
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Phagocytosis-types of cells involved, effector mechanisms, regulations
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Pathogen recognition receptors (Toll and NOD systems)
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Complement system main characteristics
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Leucocyte rolling, adhesion, leucocyte extravasation
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Lymphocyte receptors generation
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Mechanisms of antigen recognition by lymphocytes
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Antigen presenting cells (professional and non-professional)
o
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Co-stimulation as a second and third signal of T cells induction
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Phenotype and function of main effector T cell subsets
Seminars:
Characteristics of antibodies – forms, classes, structure and functions,
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Monoclonal antibodies in medicine
o
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Primary and secondary immunodeficiencies
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Regulatory T cells – characteristic and functions
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Human antibacterial, antiviral, antifungal and anti-parasite response
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Tumor stromal cells - basic tumor immunology
Tutorials:
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Cells of the immune system and antigen recognition
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The analysis of cell phenotype in immunodeficiencies, the flow cytometry
method
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Mechanisms of cytotoxicity
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Cell death as a significant process in immunology
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Components of innate immune system – the evaluation of the function of
phagocytic cells
VIII.
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Flow cytometric measurement of intracellular proteins
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Cell isolation and culture procedures in immunology
Booklist:
Basic:
1.
Abul K. Abbas, Andrew H. Lichtman: Basic Immunology, Sauders Elsevier
2.
Roderick Nairn, Matthew Helbert: Immunology for medical students, Mosby Elsevier
3.
Mark Peakman, Diego Vergani: Basic and clinical immunology, Churchill Livingstone
Elsevier
Additional:
4. Tak W. Mak, Mary E. Saunders: Primer to the immune response, Academic Press Elsevier
5. Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai: Cellular and molecular immunology,
Sauders Elsevier
6. Kenneth M. Murphy, Paul Travers, Mark Walpor
Janeway’s Immunobiology, Garland
RULES AND REGULATIONS
1. Attendance is obligatory at each lecture, tutorial and seminars.
2. Each change between the groups must be approved by the teacher.
3. Students are expected to be punctual.
4. During the classes students must have protective gloves and a lab coat.
5. In case of absence students must present a sick leave on the next classes.
6. Students should have the basic knowledge of the tutorial topics as well as have to
actively participate in the classes in order to accomplish the course.
7. Each tutorial and seminars includes a 10-point quiz, which is a form of evaluation of
students’ knowledge.
8. Each workshop is based on the individually prepared presentations. If students do
not prepare the presentations for the workshops they will receive negative points (1).
9. Students who did not take the test because of the absence need to pass it
individually no later than two weeks after return from the sick leave.
10. To pass tutorials and workshops students need to collect 60% of total points.
11. Students who receive less than 60% must take a test from knowledge presented in all
tutorials and seminars.
12. The clinical immunology course ends with a credit-grade assigned for lectures and
60% of tutorials and seminars.