Download ORGANELLES AND PROTEIN SYNTHESIS Worksheet #3

Organelles and Protein Synthesis - WS: Page 1 of 2
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ORGANELLES AND PROTEIN SYNTHESIS
Worksheet #3
NAME
SECTION
Lab Partners
A. Organelle Functions and Protein Synthesis
1) Organelle Functions:
a. Define the function of the following items and indicate if it is an organelle or not
CELLULAR
STRUCTURES:
Plasma Membrane
Ribosomes
Transport vesicles
Messenger RNA
(mRNA)
Cytoskeleton
DNA
Nucleus
Golgi Complex
Endoplasmic
Reticulum
FUNCTIONS:
Is this an
Organelle?
Organelles and Protein Synthesis - WS: Page 2 of 2
2) The Protein Synthesis Pathway
a. Follow the instructions in your lab manual to create a cell on your lab bench. Your
instructor will guide you through the steps of protein synthesis.
B. TRANSCRIPTION AND TRANSLATION
DNA & PROTEINS:
1. The nucleotide sequence for hemoglobin (one subunit) has 576 bases (nucleotides). How
many amino acids would be in this subunit?
Number of amino acids = ___________
2. Below, copy the first 15 DNA nucleotides for the Hemoglobin gene into DNA Strand 1.
Using the DNA nucleotide pairing rules, create DNA Strand 2.
DNA
Strand
1
DNA
Strand
2
3. TRANSCRIPTION: Below, transcribe the DNA Strand 1 into an mRNA sequence. Draw
circles around the mRNA codons!
mRNA
Strand
4. TRANSLATION: Using the mRNA sequence above, translate each codon into the
appropriate amino acids.
Amino
Acid
Sequence
5. DNA is located in the nucleus of the cell, but the information is used to ‘code’ for amino
acids outside of the Nucleus. Explain how the information in the DNA (gene) is moved
outside of the nucleus
6. What is a codon? What is it and what does it do?
7. What cellular organelle makes/assembles proteins? (i.e., what organelle actually
produces the polypeptide?)