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Lab 6—Roots, Stems, Leaves Willow shoot Plant Parts Monocot vs Dicot Plant organs are composed of three tissue systems: 1. Dermal tissue 2. Vascular tissue 3. Ground tissue Plant Cell Structure cell wall chloroplast nucleus central vacuole Cell Wall Structure secondary cell wall primary cell wall middle lamella Cell Wall Structure plasmodesmata Plant Cell Types • Xylem –Tracheids –Vessel elements • Phloem –Sieve-tube members –Companion cell Vascular Tissue Vascular tissue: • runs continuous throughout the plant • transports materials between roots and shoots. – Xylem transports water and dissolved minerals upward from roots into the shoots. (water the xylem) – Phloem transports food from the leaves to the roots and to non-photosynthetic parts of the shoot system. (feed the phloem) Xylem The water conducting elements of xylem are the tracheids and vessel elements. Xylem • Tracheids – Characteristics • tapered elongated cells • connect to each other through pits • secondary cell walls strengthened with lignin • dead at functional maturity – Functions • transport of water plus dissolved minerals • support Xylem • Vessel Elements –Characteristics • shorter and wider than tracheids • possess thinner cell walls than tracheids • Aligned end-to-end to form long micropipes • dead at functional maturity –Functions • transport of water plus dissolved minerals • support Water conducting cells of the xylem Phloem • Food and minerals move through tubes formed by chains of cells, sieve-tube members. –sieve plates –companion cell Phloem • Sieve-tube Members – Characteristics • living cells arranged end-to-end to form foodconducting cells of the phloem • lack lignin in their cell walls • mature cells lack nuclei and other cellular organelles • alive at functional maturity – Functions • transport products of photosynthesis Phloem • Companion Cells – Characteristics • living cells adjacent to sieve-tube members • connected to sieve-tube members via plasmodesmata – Functions • support sieve-tube members • may assist in sugar loading into sieve-tube members Food conducting cells of the phloem Ground Tissue Ground tissue fills the interior of the plant. It contains three basic cell types: Dermal tissue – Parenchyma cells – Collenchyma cells – Sclerenchyma cells Ground tissue Vascular tissue Parenchyma • Characteristics – least specialized cell type – only thin primary cell wall is present – possess large central vacuole – generally alive at functional maturity • Functions – make up most of the ground tissues of the plant – storage – photosynthesis – can help repair and replace damaged organs by proliferation and specialization into other cells Parenchyma Collenchyma • Characteristics –possess thicker primary cell walls the that of parenchyma –no secondary cell wall present –generally alive at functional maturity • Functions –provide support without restraining growth Collenchyma Sclerenchyma • Characteristics –have secondary cell walls strengthened by lignin –often are dead at functional maturity –two forms: fibers and sclereids • Functions –rigid cells providing support and strength to tissues • Two other sclerenchyma cells, fibers and sclereids, are specialized entirely in support. – Fibers are long, slender and tapered, and usually occur in groups. • Those from hemp fibers are used for making rope and those from flax for weaving into linen. – Sclereids, shorter than fibers and irregular in shape, impart the hardness to nutshells and seed coats and the gritty texture to pear fruits. Fiber Cells Sclereids Plant Growth & Development • Meristems– embryonic tissue. – These cells divide to generate additional cells. – Initials- generative cells that remain in the meristem. – Derivatives- Those that are displaced from the meristem,and continue to divide for some time until the cells they produce begin to specialize within developing tissues. Locations of Meristematic Tissues • Apical meristems: located at the tips of roots and in the buds of shoots, supply cells for the plant to grow in length. –Primary growth • initial root and shoot growth • produced by apical meristem • elongation occurs • restricted to youngest parts of the plant, i.e, tips of roots & shoots Locations of Meristematic Tissues • Lateral meristems: allow the plant to increase in girth –Secondary growth: thickening of roots and shoots. • Produced by lateral meristems • Develop in slightly older regions of roots and shoots • Examples: vascular and cork cambium. Meristems Types of Primary Meristems • Protoderm: forms dermal tissue system • Procambium: forms vascular tissue system • Ground Meristem: forms ground tissue system Primary Growth in Roots • Root Cap: covers root tip & protects the meristem as the root pushes through the abrasive soil during primary growth. – The cap also secretes a lubricating slime. • Growth in length is concentrated near the root’s tip, where three zones of cells at successive stages of primary growth are located. – zone of cell division – zone of elongation – zone of maturation Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings • The procambium gives rise to the stele, which in roots is a central cylinder of vascular tissue where both xylem and phloem develop. Stele Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings Dicot Root Monocot Root Monocot Root Anatomy cortex epidermis cortex endodermis pericycle stele pith pith phloem xylem cortex Dicot Root Anatomy endodermis pericycle epidermis cortex stele xylem phloem Plant Shoot Primary Growth of the Shoot Stem Anatomy Dicot Stem Monocot Stem Monocot Stem Anatomy sclerenchyma phloem epidermis vascular bundles ground tissue xylem parenchyma epidermis Dicot Stem Anatomy phloem cortex vascular bundle pith vascular cambium xylem Anatomy of a Tree Trunk • After several years of secondary growth, several zones are visible in a stem. Leaf Anatomy Typical Dicot Leaf X-Section Cuticle Palisade Parenchyma Epidermis Vascular bundles Guard Cells Spongy Parenchyma Stoma Typical Monocot Leaf X-Section Midvein Vein Bundle sheath cell Epidermis Phloem Xylem Bulliform Cells Stoma Leaf Stomata: Allow Gas Exchange Guard cells Stoma