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S-B-8-2_Protein Synthesis Worksheet and KEY Name PROTEIN SYNTHESIS 1. Use the molecular template to make DNA, mRNA, tRNA, and amino acids. DNA Coding Strand ATG DNA Template TAC mRNA codons AUG tRNA anticodons UAC Amino Acids met TCG AGA GCC CCG ATG GTC CAC CAA AGA GGT TTT 2. Write the mRNA codons, the tRNA anticodon, and the DNA coding strand that complements the DNA template below. Write the amino acid that each tRNA carries. DNA Coding Strand TAC DNA Template ATG mRNA codons UAC tRNA anticodons AUG Amino Acids tyr TCG AGA GCC CCG ATG GTC CAC CAA AGA GGT TTT S-B-8-2_Protein Synthesis Worksheet and KEY 3. Where does transcription take place? 4.Where does translation take place? 5. What molecule is produce in transcription? What molecule is produced in translation? 6. What signals the beginning of translation (binding of rRNA to mRNA)? 7. What halts the process of translation? 8. How many amino acids had only one codon? How many have more than one codon? 9. Why would having more than one codon for an amino acid be important? 10. What cellular organelle begins the folding and modifications of the polypeptide? 11. If the protein product is used outside the cell or in other parts of the cell, the protein is sent to the . The purpose of the carbohydrate chains added to the protein is to . S-B-8-2_Protein Synthesis Worksheet and KEY ANSWER KEY PROTEIN SYNTHESIS 1. Use the molecular template to make DNA, mRNA, tRNA, and amino acids. DNA Coding Strand ATG TCG AGA GCC CCG ATG GTC CAC CAA AGA GGT TTT DNA Template TAC AGC TCT CGG GGC TAC CAG GTG GTT TCT CCA AAA mRNA codons AUG UCG AGA GCC CCG AUG GUC CAC CAA AGA GGU UUU tRNA anticodons UAC AGC UCU CGG GGC UAC CAG CUC GUU UCU CCA AAA Amino Acids met ser arg ala gly met val glu glu arg gly phe 2. Write the mRNA codons, the tRNA anticodon, and the DNA coding strand that complements the DNA template below. Write the amino acid that each tRNA carries. DNA Coding Strand TAC DNA Template ATG mRNA codons UAC tRNA anticodons AUG Amino Acids TCG AGA GCC CCG ATG GTC CAC CAA AGA GGT TTT tyr 3. Where does transcription take place? In the nucleus 5. What molecule is produce in transcription? mRNA molecule 4. Where does translation take place? In the cytoplasm or on the rough ER What molecule is produced in translation? a polypeptide (amino acid chain) 6. What signals the beginning of translation (binding of rRNA to mRNA)? A start codon on the mRNA molecule 7. What halts the process of translation? A stop codon on the mRNA molecule and the release of the polypeptide 8. How many amino acids had only one codon? 2 How many have more than one codon? 18 S-B-8-2_Protein Synthesis Worksheet and KEY 9. Why would having more than one codon for an amino acid be important? If there is a mistake in the DNA sequence, there is less chance of the mistake to be translated to the amino acid sequence. 10. What cellular organelle begins the folding and modifications of the polypeptide? The rough endoplasmic reticulum 11. If the protein product is used outside the cell or in other parts of the cell, the protein is sent to the Golgi Apparatus . The purpose of the carbohydrate chains added to the protein is to label to where it will be sent next. . S-B-8-2_Protein Synthesis Worksheet and KEY