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Kevin Ahern's Biochemistry (BB 450/550) at Oregon State University
Kevin Ahern's Biochemistry (BB 450/550) at Oregon State University

... UDP-glucose), are ACTIVATED. Activated carriers contain a high energy between themselves (such as CoA) and the molecule they are carrying (acetyl group). The high energy of their bond is used to make possible the reaction where the molecule being carried is donated to a larger molecule. 2. There are ...
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... The equilibrium of this reaction lies far to the left under standard conditions. In cells, the concentration of OAA is extremely low (<10–6 M), pulling the malate dehydrogenase reaction towards the formation of OAA. ...
9.3 student notes
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... • The cells of most organisms transfer energy found in organic compounds, such as those in foods, to ATP. • The primary fuel for cellular respiration is glucose. Fats can be broken down to make ATP. • Proteins and nucleic acids can also be used to make ATP, but they are usually used for building imp ...
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... the absence of oxygen  Glycolysis and fermentation together only produces 2 ATP.  This is not efficient!! ...
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Chapter 9 - Slothnet
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... ways of inserting these non-canonical amino acids: either by genetic incorporation or by post-translational modification. Major advances in engineering new enzyme activities have been made by site-directed mutagenesis and directed evolution, however these methods are restricted to the use of the twe ...
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... A) All enzymes of the cycle are located in the cytoplasm, except succinate dehydrogenase, which is bound to the inner mitochondrial membrane. B) In the presence of malonate, one would expect succinate to accumulate. C) Oxaloacetate is used as a substrate but is not consumed in the cycle. D) Succinat ...
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... begins with the two-carbon Acetyl CoA and the four-carbon oxaloacetic acid to make a sixcarbon citric acid molecule. The citric acid then goes through a series of reactions where NAD+ is hydrogenated to make NADH, ADP is used to make ATP, and two carbon dioxide molecules are released. Since the two ...
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... • Chemical formula = [CH2O]N (multiples of CH2O) • e.g., C6H12O6 = glucose • Carbohydrates have many functions • Structural components of molecules (e.g., DNA, RNA), cells and tissues – cellulose is the most abundant organic substance on earth – we cannot digest it, but it is an important part of ou ...
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(PDF format, 1.73MB)

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... 6.9 The citric acid cycle completes the oxidation of organic molecules, generating many NADH and FADH2 molecules  With the help of CoA, the acetyl (two-carbon) compound enters the citric acid cycle – At this point, the acetyl group associates with a fourcarbon molecule forming a six-carbon molecul ...
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... other mitochondrial uncoupling proteins) which are activated by calcium coming in through the calcium uniporter. Also note that electron carriers can autooxidize directly to oxygen, creating oxygen radicals (Co-Q is the major site of autooxidation) with as much as 5% of resting oxygen use due to thi ...
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BIO 315 Exam I (F2014)

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Citric acid cycle



The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.
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