Oligonucleotide 5` End Labeling with Radiochemicals
... The techniques for end labeling oligonucleotides with radioisotopes have driven nucleic acid probe technology. Oligonucleotide probes can be custom made based on sequence information of the target DNA or RNA in several hours on a DNA synthesizer. Use of a DNA synthesizer eliminates the usual cumbers ...
... The techniques for end labeling oligonucleotides with radioisotopes have driven nucleic acid probe technology. Oligonucleotide probes can be custom made based on sequence information of the target DNA or RNA in several hours on a DNA synthesizer. Use of a DNA synthesizer eliminates the usual cumbers ...
Metabolism/Energy
... Has eight steps, each catalyzed by a specific enzyme Most of the energy harvested in the Krebs cycle is conserved in NADH (3 molecules) One step transfers energy to FAD, resulting in FADH2 (1 molecule) Only one molecule of ATP is produced for each “turn” of the Krebs cycle Two molecules of ...
... Has eight steps, each catalyzed by a specific enzyme Most of the energy harvested in the Krebs cycle is conserved in NADH (3 molecules) One step transfers energy to FAD, resulting in FADH2 (1 molecule) Only one molecule of ATP is produced for each “turn” of the Krebs cycle Two molecules of ...
Bio 201, Fall 2010 Test 3 Study Guide Questions to be able to
... 27. What do enzymes do to allow biological reactions to proceed? 28. How do enzymes speed up reactions? 29. How do we regulate enzyme activity? 30. Describe the structure of ATP. 31. Why is ATP so energy rich? 32. How do cells use ATP to drive endergonic reactions? 33. How do we make energy-rich ATP ...
... 27. What do enzymes do to allow biological reactions to proceed? 28. How do enzymes speed up reactions? 29. How do we regulate enzyme activity? 30. Describe the structure of ATP. 31. Why is ATP so energy rich? 32. How do cells use ATP to drive endergonic reactions? 33. How do we make energy-rich ATP ...
ch9sec1n2_2013
... INTERMEMBRANE SPACE represents _______________________ potential energy that is harnessed to make ATP. As H+ ions escape through ion channels ATP SYNTHASE back into the matrix, ________________ spins and adds a phosphate to ADP to ATP form _______ ...
... INTERMEMBRANE SPACE represents _______________________ potential energy that is harnessed to make ATP. As H+ ions escape through ion channels ATP SYNTHASE back into the matrix, ________________ spins and adds a phosphate to ADP to ATP form _______ ...
Biomolecule SG_answers
... Saturated fats (butter, dairy products, meat) are fats which are evenly filled out with hydrogen, which remains solid at room temperature. UNSATURATED - COME FROM PLANTS, LIQUID AT ROOM TEMPERATURE, MISSING HYDROGENS, DOUBLE BONDS Unsaturated fatty acids that your body can’t manufacture and, therefo ...
... Saturated fats (butter, dairy products, meat) are fats which are evenly filled out with hydrogen, which remains solid at room temperature. UNSATURATED - COME FROM PLANTS, LIQUID AT ROOM TEMPERATURE, MISSING HYDROGENS, DOUBLE BONDS Unsaturated fatty acids that your body can’t manufacture and, therefo ...
Name Class Date Reviewing Key Concepts Identifying On the lines
... 1. the main source of energy for living things 2. help carry out chemical reactions 3. important parts of biological membranes 4. contain hydrogen, oxygen, nitrogen, phosphorus, and carbon 5. transport substances in and out of cells 6. composed of amino acids 7. sugar and starches 8. store and trans ...
... 1. the main source of energy for living things 2. help carry out chemical reactions 3. important parts of biological membranes 4. contain hydrogen, oxygen, nitrogen, phosphorus, and carbon 5. transport substances in and out of cells 6. composed of amino acids 7. sugar and starches 8. store and trans ...
Cellular Respiration
... - After CO2, NADH, FADH2, and ATP is released, Oxaloacetate is recycled back into the cycle to combine with another Acetyl CoA - **Remember the cycle occurs twice! (once for each Acetyl CoA molecule) ** Produces some chemical energy in the form of ATP but most of the chemical energy is in the form o ...
... - After CO2, NADH, FADH2, and ATP is released, Oxaloacetate is recycled back into the cycle to combine with another Acetyl CoA - **Remember the cycle occurs twice! (once for each Acetyl CoA molecule) ** Produces some chemical energy in the form of ATP but most of the chemical energy is in the form o ...
Biochemistry Test Review KEY
... 13. What is a common element found in all organic compounds? Carbon 14. What effect does pH levels have on a certain enzyme? Slows or speeds up rate of reaction, as well as the modifies the enzyme’s shape 15. Describe the active site of an enzyme. It is a small port in an enzyme where substrate mole ...
... 13. What is a common element found in all organic compounds? Carbon 14. What effect does pH levels have on a certain enzyme? Slows or speeds up rate of reaction, as well as the modifies the enzyme’s shape 15. Describe the active site of an enzyme. It is a small port in an enzyme where substrate mole ...
How Cells Harvest Energy
... #1: Glucose is converted to glucose6-phosphate in a phosphorylation reaction ...
... #1: Glucose is converted to glucose6-phosphate in a phosphorylation reaction ...
PowerPoint
... • Water is split into hydrogen ions, electrons, and oxygen (O2) through the process called the electron transport chain. • The light energy is now converted to chemical energy, which is temporarily stored in ATP and NADPH (energy molecules). • The O2 diffuses out of the chloroplasts ...
... • Water is split into hydrogen ions, electrons, and oxygen (O2) through the process called the electron transport chain. • The light energy is now converted to chemical energy, which is temporarily stored in ATP and NADPH (energy molecules). • The O2 diffuses out of the chloroplasts ...
Book Problems Chapter 2
... (a) ATP + H2O → ADP + Pi The transporter must include a cytosolic nucleotide binding site that changes its conformation when its bound ATP is hydrolyzed to ADP. This conformational change must be communicated to the membrane-spanning portion of the protein, where the transported substrate binds. (b) ...
... (a) ATP + H2O → ADP + Pi The transporter must include a cytosolic nucleotide binding site that changes its conformation when its bound ATP is hydrolyzed to ADP. This conformational change must be communicated to the membrane-spanning portion of the protein, where the transported substrate binds. (b) ...
Photosynthesis
... • Water is split into hydrogen ions, electrons, and oxygen (O2) through the process called the electron transport chain. • The light energy is now converted to chemical energy, which is temporarily stored in ATP and NADPH (energy molecules). ...
... • Water is split into hydrogen ions, electrons, and oxygen (O2) through the process called the electron transport chain. • The light energy is now converted to chemical energy, which is temporarily stored in ATP and NADPH (energy molecules). ...
Bio 226: Cell and Molecular Biology
... •Insensitive to Cyanide, Azide or CO •Sensitive to SHAM (salicylhydroxamic acid,) •Also found in fungi, trypanosomes & Plasmodium ...
... •Insensitive to Cyanide, Azide or CO •Sensitive to SHAM (salicylhydroxamic acid,) •Also found in fungi, trypanosomes & Plasmodium ...
Gluconeogenesis: Objectives
... a. Adipose Tissue lacks Glycerol Kinase so the glycerol that is liberated in the break down of fatty acids cannot be phosphorylated. i. Cannot make DHAP ii. Must have GLUCOSE if want to make it in adipose tissue 13. Explain how amino acids can serve as precursors for the synthesis of glucose. a. Ami ...
... a. Adipose Tissue lacks Glycerol Kinase so the glycerol that is liberated in the break down of fatty acids cannot be phosphorylated. i. Cannot make DHAP ii. Must have GLUCOSE if want to make it in adipose tissue 13. Explain how amino acids can serve as precursors for the synthesis of glucose. a. Ami ...
Cellular Respiration - Chandler Unified School District
... • Let’s first look at the following energy capturing processes that you see in everyday life. An incandescent light bulb is about 5% efficient Electricity generated from coal is about 21% efficient The most efficient gasoline combustion engine in cars is about 23% efficient. ...
... • Let’s first look at the following energy capturing processes that you see in everyday life. An incandescent light bulb is about 5% efficient Electricity generated from coal is about 21% efficient The most efficient gasoline combustion engine in cars is about 23% efficient. ...
Mitochondria
... Characteristics: • Requires the presence of Oxygen (aerobic) • takes place in the inner membrane of the mitochondria. • 2FADH2+ 6NADH2+ 2 ATP • The electrons of NADH and FADH2 are transferred to the respiratory chain ...
... Characteristics: • Requires the presence of Oxygen (aerobic) • takes place in the inner membrane of the mitochondria. • 2FADH2+ 6NADH2+ 2 ATP • The electrons of NADH and FADH2 are transferred to the respiratory chain ...
I ADDED TISSUES JUST IN CASE!!! APHY 101, Lecture 4
... 5. Steps a. Hydrogen bonds of DNA break & strands separate b. RNA Polymerase builds mRNA using DNA as template c. mRNA transcript is transported to ribosomes in cytoplasm ...
... 5. Steps a. Hydrogen bonds of DNA break & strands separate b. RNA Polymerase builds mRNA using DNA as template c. mRNA transcript is transported to ribosomes in cytoplasm ...
Protein Turnover and Amino Acid Catabolism
... Hemoglobin lasts as long as a red blood cell. Υ-Crystallin (eye lens protein) lasts as long as the organism does. ...
... Hemoglobin lasts as long as a red blood cell. Υ-Crystallin (eye lens protein) lasts as long as the organism does. ...
Chapter 6, Section 3
... Enzymes act on specific substrates ◦ Substrate: substance that the enzyme breaks down Each substrate fits into the active site. (Like a lock & key) ...
... Enzymes act on specific substrates ◦ Substrate: substance that the enzyme breaks down Each substrate fits into the active site. (Like a lock & key) ...
Citric acid cycle
The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.