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DNA Technology
DNA Technology

Advances in Genetics
Advances in Genetics

... • The process of selecting organisms with desired traits to be parents of the next generation is called selective breeding • The corn we use today is a great example! • 2 techniques • Inbreeding • hybridization ...
Notes_DNA Replication_teacher
Notes_DNA Replication_teacher

... nucleus except during cell division (mitosis); it will get destroyed by enzymes in the cytoplasm.  enzymes and freenucleotides are available for replication in the nucleus. Part 1 Initiation:  an enzyme called helicase attaches to DNA and breaks the Hydrogen bonds to unwind and separate the comple ...
summing-up - Zanichelli online per la scuola
summing-up - Zanichelli online per la scuola

... small circular DNA molecules present in the cytoplasm of bacteria. They can be transmitted from one bacterium to another through the mechanism of conjugation. There are several types of plasmids. ...
Reading Study Guide B
Reading Study Guide B

... Review Traits are passed on through genes. ...
Molecular Genetics Outcome Checklist
Molecular Genetics Outcome Checklist

... _____ I can explain how, in general, restriction enzymes cut DNA molecules into smaller fragments based on a specific nucleotide sequence, leaving “sticky ends”. _____ I understand the purpose and function of ligases. _____ I can explain how restriction enzymes, ligases, and other DNA technology ca ...
ChIP-seq
ChIP-seq

... • If we have the “right” antibody, we can extract (“immunoprecipitate”) from living cells the protein of interest bound to the DNA • And - we can try to identify which were the DNA regions bound by the protein • Can be done for transcription factors • But can be done also for histones - and separate ...
7.1 - DNA Structure
7.1 - DNA Structure

... 7.1.3 - State that nucleosomes help to supercoil chromosomes and help to regulate transcription During supercoiling, the DNA is condensed by a factor of x15000. The histones are responsible for the packaging of DNA at the different levels. The metaphase chromosome is an adaption for mitosis and mei ...
name period ______ date
name period ______ date

... 3. What is the name of the enzyme that breaks the nitrogen bases apart to get them ready for replication? 4. What is the name given to the point where replication starts on a DNA molecule? 5. How does the replicated daughter molecule of DNA compare to the parent molecule of DNA? 6. What would the co ...
Nucleic acid review sheet
Nucleic acid review sheet

... What is the name of the sugar in RNA nucleotides? Underline the first letter. ...
Microbial Genetics
Microbial Genetics

... • Initiator proteins (IP; about 30) bind at repetitive sequences within the OriC site. • DNA winds around IP-complex; induces separation of strand at adjacent AT rich site. • Single strand binding proteins & helicases attach. • Formation of two replication forks that open in opposite ...
2-3 DNA to Proteins - Lighthouse Christian Academy
2-3 DNA to Proteins - Lighthouse Christian Academy

... Genes – All of your DNA is organized into 23 chromosomal pairs. These are like encyclopedias that contain instructions on how to make all the proteins in a body part. A single gene is the instructions for how to make a single protein. ...
here
here

... DNA profiling is a technique that allows an individual’s genes to be visualised, this allows someone's genetic makeup to be compared to known genes to see if they too have it. This technique can be used to identify genetic disorders in individuals or match DNA samples to individuals. We usually sam ...
DNA – Chromosomes - Genes - Science
DNA – Chromosomes - Genes - Science

Document
Document

... Saltwater fish remove extra salt from their body by active transport through the gills. What is the result of this activity B Water balance is maintained in the blood. C The rate of energy production is decreased. D The cell membrane becomes less permeable to water. ...
Honors Biology
Honors Biology

... 10. Explain how mutations lead to cystic fibrosis and sickle cell anemia. (Predict the effect various changes in DNA will have on the transcribed RNA, on the synthesized protein product, on the cell and on the affected individual in sickle cell anemia and in cystic fibrosis.) Structure/function rela ...
Principles and Practices of Biosafety
Principles and Practices of Biosafety

... not require additional safety measures. In cases where these sequences are not characterized, a situation that is typically encountered when a library of genomic DNA of an organism is being established, a higher BSL will be required. Cloning of genes coding for proteins that have potential pharmacol ...
Biotechnology Need To Know List
Biotechnology Need To Know List

... How a gene marker is used to distinguish transformed bacteria When transformation of a plant cell is considered successful The relationship between genetic engineering and transgenic organisms Sex determination in humans What a pedigree allows a researcher to do Inheritance of PKU, ABO blood type, s ...
CentralDogmaNotes
CentralDogmaNotes

... • The information content of DNA is in the form of specific sequences of nucleotides • The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins • Gene expression, the process by which DNA directs protein synthesis, includes two stages: transcription and transl ...
SBI 3CI Diagnostic Quiz October 10, 2014 – Microbiology Name
SBI 3CI Diagnostic Quiz October 10, 2014 – Microbiology Name

... Vaccines are considered a form of passive immunity. Recombinant DNA is DNA that has been spliced open and strands of DNA added in. ...
Recombinant Technology
Recombinant Technology

... microbiological selection and screening procedures to allow investigators to isolate a gene that represents as little as 1 part in a million of the ...
Biotechnology - Cobb Learning
Biotechnology - Cobb Learning

... Biotechnology ...
chapter 12 test review key
chapter 12 test review key

... 7 ___C___“Discovered” that DNA is the genetic material that can cause bacteria to make viruses instead of new bacteria. 8 ___B___“Discovered” that DNA is the factor that caused one bacterium to transform into another strain. 9 ___A___“Discovered” that some unknown factor causes transformation to occ ...
Document
Document

... 4. In the following diagrams, the vertical lines represent EcoRI restriction sites. An asterisk over the site represents a polymorphism (presence or absence of the site in individuals) in the population. The double arrow represents the boundaries of the cloned DNA used in the Southern blot analysis. ...
DNA Sequencing
DNA Sequencing

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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