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Molecules to Eye Color - Springfield School District
Molecules to Eye Color - Springfield School District

...  3 main differences between DNA and RNA  1. Ribose sugar (not deoxyribose)  2. Has U (uracil) instead of T (thymine)  3. Single strand (not double) ...
[Type the document title] Microbial Genetics Molecular biology is the
[Type the document title] Microbial Genetics Molecular biology is the

... with the interactions between the various systems of a cell, including the interrelationship of DNA, RNA and protein synthesis and learning how these interactions are regulated. ►Molecular genetics is the field of biology which studies the structure and function of genes at a molecular level. ...
Genetic Engineering
Genetic Engineering

... 1. Genetic engineering – making changes in the DNA code. a. DNA extraction – remove DNA from a cell b. DNA cutting / splicing – DNA is cut into smaller fragments using restriction enzymes. c. DNA separation – Gel electrophoresis is used. The smallest fragments travel the furthest. * Recombinant DNA ...
Introduction to DNA - University of Dayton
Introduction to DNA - University of Dayton

... Introduction to DNA ...
Biotechnology Unit Test Review
Biotechnology Unit Test Review

... nitrogen base sequences and make zig-zag cut to make “sticky ends”) 3. Gene cloning – A gene is inserted into a bacteria. Then, many copies of the gene are made when the bacteria divide. 4. DNA ligase – Enzyme used to join the “sticky ends” of a recombinant DNA 5. Gel electrophoresis – Technique use ...
Mitosis and Asexual Reproduction
Mitosis and Asexual Reproduction

... Eukaryotic: a domain of organisms having cells each with a distinct nucleus within which the genetic material is contained along with other membrane-bound organelles. Prokaryotic: any organism having cells in each of which the genetic material is in a single DNA chain, not enclosed in a nucleus. The ...
Misconceptions relating to DNA and RNA
Misconceptions relating to DNA and RNA

... Amino acids enter through the nuclear pores similar to nucleotides Amino acids are aligned with mRNA strand through trial and error similar to the jigsaw activity That DNA, in this helical structure, will stay straight and upright, that it won’t coil up around itself and other DNA molecules in order ...
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids

... genotype different from either parent (exchange of DNA between different genes) 1. occurs during meiosis as crossing over between homologous chromosomes 2. genetic recombination (homologous recombination) is the most common form, involving reciprocal exchange between a pair of homologous DNA sequenc ...
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No Slide Title

... Input DNA is converted into long tandem repeats chromosome ...
Biotechnology Genetic Engineering and Recombinant DNA
Biotechnology Genetic Engineering and Recombinant DNA

... Biotechnology- technology that uses biological systems, living organisms, or products made from living organisms to make or modify products or processes for specific use ...
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... Biotechnology- technology that uses biological systems, living organisms, or products made from living organisms to make or modify products or processes for specific use ...
2 - Blue Valley Schools
2 - Blue Valley Schools

... know the general goal of the mitosis and the other stages of the cell cycle. 3. You should know the forms that DNA takes during the cell cycle and be familiar with the structures associated with DNA coiling. 4. You should be able to name those scientists who contributed to our knowledge of DNA’s fun ...
AP Bio Molecular Genetics Review Sheet
AP Bio Molecular Genetics Review Sheet

... Tobacco Mosaic Virus has RNA rather then DNA. If you mixed RNA from TMV is mixed with proteins from a related DNA virus. If the resulting hybrid virus were to infect a cell what would the offspring viruses be like? What is the function of DNA polymerase? What mechanism ensures that DNA replication i ...
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Basics Terms of Life Science Cells

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Dr T-J’s Minilecture - Susquehanna University
Dr T-J’s Minilecture - Susquehanna University

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... recombinant plasmids in a particular colony of bacteria • cDNA libraries contain all the gene that there are cDNA for ...
EOC Review Part 4
EOC Review Part 4

DNA Study Guide 1. The sides of a DNA molecule are made up of
DNA Study Guide 1. The sides of a DNA molecule are made up of

... 26. What trait is controlled by a gene with multiple alleles? ______________________________________________ 27. Why does height have such a wide variety of phenotypes? ___________________________________________ 28. Human eyes come in a variety of colors. Explain why eye color is not likely control ...
Epigenetics - Hospital Melaka Department of Medicine Haematology
Epigenetics - Hospital Melaka Department of Medicine Haematology

...  A 'rough draft' of the genome was finished in 2000, announced jointly by U.S. President Bill Clinton and the British Prime Minister Tony Blair on June 26, ...
For teachers: Get four colours of beads or rubber bands. You can
For teachers: Get four colours of beads or rubber bands. You can

... The instructions in our DNA are written in a chemical code. We represent these chemicals by four letters: ...
DNA_LAdders_files/StoS 100bp DNA Ladder flyer new
DNA_LAdders_files/StoS 100bp DNA Ladder flyer new

... 11 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 bp. The 500 and 1,500 bp bands have increased intensity to serve as referce points. The approximate mass of DNA in each band is provided (0,5ug a load) for a ...
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Italian Association for Cancer Research NETWORK OF

... The overall goals of the Network are: (a) to create a network of researchers involved in the identification of relevant interactions between genes and the environment through studies of molecular epidemiology in Italy; (b) to rationalize and improve the quality of laboratory measurements by referrin ...
AP Biology
AP Biology

Slide 1 - KU CTE
Slide 1 - KU CTE

E. coli
E. coli

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< 1 ... 741 742 743 744 745 746 747 748 749 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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