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Changes in DNA can produce Variation
Changes in DNA can produce Variation

Gene Technology
Gene Technology

... Examined over 6 bill. Nucleotide bases in 20-30,000 genes! Location of genes on chromosomes established Once you know where the genes are, you can change them!! ...
Research Questions
Research Questions

... methionine (Met), and tryptophan (Trp).Hydrophobic amino have side-chains that do not like to reside in an aqueous environment. For this reason, one generally finds these amino acids buried within the hydrophobic core of the protein, or within the lipid portion of the membrane. Hydrophilic amino aci ...
DNA Replication
DNA Replication

... entire strand is copied  Result is two strands of DNA  Semi-Conservative Replication - Each strand is 50% new and 50% old DNA ...
2. DNA Replication and Repair
2. DNA Replication and Repair

... The Process of DNA Replication Separating the DNA Strands  replication begins when a protein binds to a specific site on the DNA molecule called the replication origin  the linear DNA of eukaryotes have more than one replication origin, while the DNA of prokaryotes have only one  an enzyme (DNA h ...
recBCD
recBCD

... recBCD Pathway of Homologous Recombination •RecBCD binds an end of linear dsDNA •RecD helicase travels on the strand with a 5' end and RecB on the strand with a 3' end •RecB is slower than RecD, so that a ssDNA loop accumulates ahead of RecB •This produces DNA structures with two ss tails and one s ...
Mutations & Genetic Engineering
Mutations & Genetic Engineering

... • Example: Williams Syndrome ...
suggested essay-type questions for next exam
suggested essay-type questions for next exam

... pairs, thereby unwinding the supercoils. However, the linking number of the DNA is not changed! Explain the physical basis for the ability of ethidium bromide to “unwind” these supercoils. (You will have to look at the definition of the linking difference. In this definition, Lo refers to the linkin ...
Micro Quiz #3R Stu F2011 - the Biology Scholars Program Wiki
Micro Quiz #3R Stu F2011 - the Biology Scholars Program Wiki

... d. Circle one of the deoxyribose molecules. e. Indicate one of the phosphodiester bonds with an arrow. f. Would the strand shown be a leading strand during replication? ...
What`s the Big Deal About DNA?
What`s the Big Deal About DNA?

... Explore the Web Quest online and use the space below to record what you learn. ...
Mutations and DNA Technology Notes
Mutations and DNA Technology Notes

... - Affects large sections of DNA rather than smaller sections. - Portions of a chromosome (s) can be added, deleted or reversed. Example: Down Syndrome (Trisomy 21) - Child has 47, instead of 46 chromosomes. * What are some characteristics of someone with Trisomy 21? ...
Molecular_Evolution
Molecular_Evolution

... The Genome: smaller than we once thought • The collection of all the DNA in the cell is referred to as the genome. • We now know that most of the DNA does not code for amino acid sequences • Non-coding segments guide translation and are called introns • Coding segments are called exons ...
verbal quiz genetics 2017
verbal quiz genetics 2017

... 17. So the sequence of bases in our DNA determines the sequence of / Amino acids in a protein 18. Usually a gene is the code or instruction for making one / Protein 19. Protein synthesis occurs at the / Ribosome 20. DNA can’t leave the nucleus so / mRNA copies the genetic code and brings it to ribos ...
DNA notes File
DNA notes File

...  Mutations in the _____________ may not be as serious  Mutations in _____________ mean that the mutation is permanent.  Mutations bring ___________ to a species.  Mutations can be ________________ and _____________ ...
Changes in DNA can produce Variation
Changes in DNA can produce Variation

DNA-drug interactions and charge transfer processes in DNA.
DNA-drug interactions and charge transfer processes in DNA.

... Some organic molecules can bind to DNA and thus interfere with DNA replication, transcription and gene expression process, or even direct nucleic acid cleavage. These small molecules can thus act as therapeutic agents in cancer cure. These drug molecules can bind to DNA by different mechanisms. The ...
Biotechnology Part 3 Outline
Biotechnology Part 3 Outline

... Bacterial Cloning Process A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These en ...
6 Day 9 Biotechnology Part 3 Outline
6 Day 9 Biotechnology Part 3 Outline

... Bacterial Cloning Process A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These en ...
DNA Replication
DNA Replication

... Genome = All of the genetic material (DNA) in a cell.  Prokaryotic cell has only one genome located in the nuclear area.  Eukaryotic cell has 2 genomes  Nuclear genome  Mitochondrial genome  If not specified, “genome” usually refers to the nuclear genome. ...
Document
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... 12. How many amino acids are coded for by the following sequence of DNA? _______ ATCGACTTAGGC 13. Explain why many different proteins can be created from only 23 amino acids. ...
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Human Genome - BEHS Science

... in an enzyme within a specific cell in the immune system. In a gene therapy for this disorder the cells of the immune system are separated from blood samples and the functional gene is added to when the drawback with most current forms of gene therapy is that the gene does not always stay active for ...
Sc9 - a 3.1(teacher notes)
Sc9 - a 3.1(teacher notes)

... These variations in forms are called alleles. The ultimate combination of the chromosome pair is what makes the variation possible - combining the different variations of different characteristics to create a unique variation. ...
Greatest Discoveries with Bill Nye: Genetics
Greatest Discoveries with Bill Nye: Genetics

... 5. How many of Mendel’s genetic factors are contributed by each parent? 6. What is a Mendelian trait? Genes are Located on Chromosomes: 7. What organism was used by Thomas Hunt Morgan? 8. Why did Morgan choose these organisms? 9. What was the mutation in the flies that Morgan first noticed? 10. What ...
DNA Structure and Function
DNA Structure and Function

... B. a type of molecule that performs the main functions of cells C. a type of molecule that speeds up the rate of a chemical reaction D. a type of molecule that determines the traits that an individual inherits ...
Replication of chromosomal DNA
Replication of chromosomal DNA

... Chromosomal DNA: doublestranded; circular; haploid. Extrachromosomal genetic elements Plasmids (autonomously selfreplicating) Phages (bacterial viruses) Transposons (DNA sequences that move within the same or between two DNA molecules) ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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