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Big Picture wkst
Big Picture wkst

... _____ 6. DNA is contained in a different way in prokaryotic cells than it is in eukaryotic cells because a. eukaryotes are single-celled organisms. b. only eukaryotic cells have a cell membrane. c. DNA is found only in eukaryotic cells. d. prokaryotic cells lack a nucleus. _____ 7. Carbon-based mole ...
Gene Technology - Byron Senior High School
Gene Technology - Byron Senior High School

... – Replacing defective genes in humans with a copy that works (gene therapy) – Forensics – criminal investigations ...
Leaving Cert Biology Notes - Genetics Definitions
Leaving Cert Biology Notes - Genetics Definitions

... Same structure / modified for different functions e.g. Pentadactyl limb – arm in human wing in bird, flipper in whale Identical / alleles [accept identical genes] ...
PE #8 DNA Structure, Biotechnology, and its use in Conservation
PE #8 DNA Structure, Biotechnology, and its use in Conservation

The Wild World of Biotechnology!! Applications Genetic
The Wild World of Biotechnology!! Applications Genetic

... A DNA sample is cut with restriction enzymes. The cut up DNA is placed in one end of a gel and electricity is passed through the gel Because DNA carries a negative charge the electric current is able to carry the DNA through the gel The smallest pieces of DNA move the furthest ...
Review of relevant topics prior to “Linkage” lectures
Review of relevant topics prior to “Linkage” lectures

... DNA as it exists in the cell- normally vs. metaphase; w/ respect to chromosomes ...
Biochem Option (D)
Biochem Option (D)

... 1.00 g of cereal raises the temperature of 400. cm3 of water in an insulated food calorimeter from 23.7̊C to 33.4̊C. Calculate the energy value per gram of the cereal, assuming the heat capacity of a calorimeter is negligible and given the specific heat of water = 4.18 J/g ̊C. ...
Genetic Engineering
Genetic Engineering

... The simple addition, deletion, or manipulation of a single trait in an organism to create a ...
DNA polymerase I
DNA polymerase I

... catalyzes DNA synthesis at replication fork in 5’ to 3’ direction and only adds nucleotides at 3’ end ...
E._coli_Topo_IV
E._coli_Topo_IV

BamHI - Courses
BamHI - Courses

... End-modifying enzymes – add labels and make compatible ends for further manipulation http://www.neb.com/nebecomm/products/categories.asp ...
Biotechnology
Biotechnology

... technology are fairly simple.  We take, for example, the gene for insulin production in humans and paste it into the DNA of Escherichia coli, a bacterium that inhabits the human digestive tract. ...
How can a four "letter" code provide information that determines
How can a four "letter" code provide information that determines

The DNA connection - Somerset Academy North Las Vegas
The DNA connection - Somerset Academy North Las Vegas

...  The main function of genes is to control the production of proteins in an organism’s cells. Proteins help to determine the size, shape, color, and many other traits of an organism.  Genes and DNA: recall;  chromosomes are mostly DNA.  DNA has four different nitrogen basis (A adenine, T thymine, ...
Genetics - FAQ`s - El Camino College
Genetics - FAQ`s - El Camino College

... primary carrier of genetic (hereditary) information. It’s made up of nucleic acids, which consist of phosphates, sugars and four chemical bases (adenine, thymine, cytosine, and guanine). WHAT IS A CHROMOSOME? A threadlike structure found in the nucleus of the cell that contains the hereditary materi ...
ib biology………………
ib biology………………

... TERMS TO KNOW: Pathogenic — disease causing organism. Lytic cycle - reproductive cycle of virus. Virus attaches to host cell, injects its hereditary material into the host cell, host cell reproduces new virus particles and host cell bursts releasing new virus particles. Nucleotide - monomer of DNA a ...
Mutations Can Change the Meaning of Genes
Mutations Can Change the Meaning of Genes

... How Mutations Affect Genes Mutation: any change in the nucleotide sequence of DNA  Types of Mutations:  Base substitutions: replacement of one nucleotide w/ another. May or may not affect protein  Base deletions & Base insertions:  May be more harmful b/c all subsequent codons will be altered ...
AACR and other questions to be used as extra credit at end of 2150
AACR and other questions to be used as extra credit at end of 2150

... 1. The following is a DNA sequence. The coding sequence of the gene is in bold and italicized and the promoter is underlined. DNA 5’ T G*A A G G A A T T A T A A T A C G A C C A T G A T G T A C G C A T A A A C G T 3’ A mutation occurs in which a base (T) is inserted into the DNA sequence after the G, ...
26.1 and 26.2 Notes - Westgate Mennonite Collegiate
26.1 and 26.2 Notes - Westgate Mennonite Collegiate

... b. Gene Cloning i. Production of many identical copies of a single gene ii. Used to produce the gene’s protein product (e.g. insulin), or to alter the phenotype of an individual iii. Gene therapy: When cloned genes are used to modify a human iv. Transgenic organisms: organisms with foreign DNA or ge ...
Genetics and Genetic Engineering
Genetics and Genetic Engineering

... carriers of genes made up of DNA are located in cell nucleus ...
Name
Name

... 8. What is DNA replication? DNA unzips and the nitrogen bases that are floating in the nucleus pair up with each half of the DNA molecule. One DNA strands becomes two . 9. One section of a strand of a DNA strand has the base sequence AGATTC. What is the base sequence on the other strand? TCTAAG ...
genetics i - Indian School Al Wadi Al Kabir
genetics i - Indian School Al Wadi Al Kabir

... Infer any 3 properties of genetic code with examples from the above information 14. i) Why does DNA replication occur in small replication occur in small replication forks and not in its entire length? ii) Why is DNA replication continuous and discontinuous in a replication fork? iii) Explain the im ...
Human Genome
Human Genome

...  Scientists can read the base sequence in DNA from ...
The genetic engineers toolkit
The genetic engineers toolkit

Gene rearrangements occur via various mechanisms
Gene rearrangements occur via various mechanisms

... In meiosis and mitosis, recombination occurs between similar molecules (homologs) of DNA. In both meiotic and mitotic cells, recombination between homologous chromosomes is a common mechanism used in DNA repair. Genetic recombination and recombinational DNA repair also occurs in bacteria and archaea ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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