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DNA - NylandBiology2012-2013
DNA - NylandBiology2012-2013

... 13. What enzyme unwinds are unzips the parent strand? ________________ 14. What enzyme synthesizes the new DNA strand? _______________________ 15. The two sides of the DNA helix are held together by ________________________ 16. Write out the complete name for DNA: __________________________________ ...
Eukaryotic Gene Expression
Eukaryotic Gene Expression

... • DNA methylation; the addition of methyl groups to DNA • essential for inactivation of the DNA – Inactive genes in a cell are methylated – Epigenetic memory due to methylating enzymes that methylate the new daughter strand the same as the parent strand. • Can be passed on in repro ...
Genetic Engineering
Genetic Engineering

... Genetic Engineering the manipulation of living organisms for human use Chapter 13 ...
Structure and Role of DNA Genetic and DNA Genetics
Structure and Role of DNA Genetic and DNA Genetics

... o DNA- carries genetic information and contains instructions for cellular activity and protein production. o Nn Eukaryotes DNA is in nucleus in coiled structures(chromosomes) Prokaryotes DNA is attached to cell membrane or floats freely in the cytoplasm Endosymbiont theory- mitochondria and chloropl ...
Genetics 1
Genetics 1

... physical and mental traits of their parents or ancestors i.e. certain traits are transmitted from one generation to the next. Genetic information is carried on the DNA molecule as a gene. Gene: is the unit of heredity found on a chromosome, and is an instruction (code) to the cell to make a particul ...
Semester Exam Review
Semester Exam Review

... What happens in mitosis? Meiosis? What is the difference between the two? Be thorough What is the difference between diploid and haploid What makes up a chromosome? When does DNA replication occur? Describe cytokinesis in plant and animal cells What is a karyotype? What does it show? What is nondisj ...
Characteristics of Living Things
Characteristics of Living Things

... o DNA is a code for building proteins ...
14-3 Human Molecular Genetics
14-3 Human Molecular Genetics

... You suspect you might be carrying recessive alleles for a genetic disorder like cystic fibrosis or TaySachs disease, how can you find out??? ...
ANSWER KEY BIO SOL Review 16 - DNA - RNA
ANSWER KEY BIO SOL Review 16 - DNA - RNA

... carrying coded information from the nucleus? a. mRNA b. The ribosomes c. ATP d. The cell membrane 11. (2005-13) Tissue samples taken from the heart and stomach of a grasshopper would be expected to have the same — a. metabolic rates b. cell shape c. DNA d. cell size 12. (2003-9) Which of the followi ...
BIO SOL Review 16
BIO SOL Review 16

... carrying coded information from the nucleus? a. mRNA b. The ribosomes c. ATP d. The cell membrane 11. (2005-13) Tissue samples taken from the heart and stomach of a grasshopper would be expected to have the same — a. metabolic rates b. cell shape c. DNA d. cell size 12. (2003-9) Which of the followi ...
Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the
Part I, for Exam 1: 1. Based on Chargaff`s rules, which of the

... A) a number of conveniently located recognition sites for restriction enzymes. B) a number of palindromic sequences near the EcoRI site, which permit the plasmid to assume a conformation that protects newly inserted DNA from nuclease degradation. C) a replication origin, which permits it to replicat ...
Modeling DNA
Modeling DNA

... Why  was  the  work  of  Rosalind  Franklin  so  important  to  the  research  of  Watson  and  Crick?   ...
DNA/Protein Synthesis Study Guide
DNA/Protein Synthesis Study Guide

... What type of bond attaches a new amino acid to a polypeptide chain? By what process does a tRNA molecule attach onto mRNA during translation? ...
DNA and Protein Synthesis
DNA and Protein Synthesis

... • Examples of proteins: hormones, enzymes, neurotransmitters, receptors, components of tissue. • Proteins are composed of amino acids. • Codons are a sequence of three bases that code for a specific amino acid. ...
Genomics - West High School
Genomics - West High School

... What makes Genomics a new way to study biology? Genomics: Using tools to study all the genes in an organism (the entire genome) simultaneously once its sequence is known. (~ 190 organisms as of March ...
DNA REVIEW SHEET (answer in COMPLETE sentences on another
DNA REVIEW SHEET (answer in COMPLETE sentences on another

... Describe the hydrogen bonding between the various nitrogen bases. What molecules make up the backbone of the DNA molecule? What does the term semiconservative mean? What about antiparallel? Describe the function of each enzyme associated with DNA: helicase, DNA polymerase, DNA ligase, topoisomerase, ...
ch 20 study guide: dna technology
ch 20 study guide: dna technology

... Liga - = bound, tied (DNA ligase: a linking enzyme essential for DNA replication) Electro - = electricity (electroporation: a technique to introduce recombinant DNA into cells by applying a breif electrical pulse to a solution containing cells) Poly - = many; morph - = form (Single nucleotide polymo ...
Genetic Engineering
Genetic Engineering

... trial and error. Involves the “isolation, manipulation and reintroduction of DNA into cells or model organisms, usually to express a protein’’. DNA taken from one organism and inserted (transformed) into another (transgenic) organism Heritable, directed alteration of an organism. Altering DNA or add ...
DNA RNA Protein Hwk KEY
DNA RNA Protein Hwk KEY

... 8. … A scientist uses biotech methods to insert a human gene into bacterial cells, hoping the cells will express it and synthesize functional human protein. Instead, the protein produced is found to contain many fewer amino acids and doesn't work. What could have gone wrong? Perhaps the human gene c ...
abstract form
abstract form

... The purpose of present study was the detection of the involvement of telomeric repeats in DNA damage induced by cytostatic bleomycin (BLM). The investigation was performed with Comet-FISH, combination of Comet-assay (single cell gel electrophoresis) with FISH (Fluorescent In Situ Hybridization) tech ...
Recombinant DNA Technology
Recombinant DNA Technology

... A. Know the gene of interest and the species carrying that gene B. Need a species that can rapidly replicate its DNA and divide C. Need a species containing many restriction sites on its DNA D. Need restriction enzymes—these enzymes are able to cut double stranded DNA molecules at a specific nucleot ...
Genetic Engineering
Genetic Engineering

... • A spider’s silk-making gene has been inserted into a goat, which then produces silk in its milk ...
Genetic engineering - Mad River Local Schools
Genetic engineering - Mad River Local Schools

... GENETIC ENGINEERING Unit 6 ...
week2
week2

... to make RNA and then a corresponding protein. Parts of the DNA sequence are used to directly transcribe RNA Part of the DNA sequence help with the Expression ...
DNA: Sample Storage - Sacramento County District Attorney
DNA: Sample Storage - Sacramento County District Attorney

< 1 ... 738 739 740 741 742 743 744 745 746 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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