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Gene Expression Vocabulary
Gene Expression Vocabulary

... 9. Messenger RNA: carries hereditary information from DNA and delivers it to the site of translation 10. Transfer RNA: acts as an interpreter molecule, translating mRNA sequences into amino acid sequences 11. Ribosomal RNA: help build proteins; they function at the sites of translation 12. Codons: t ...
Study Guide: The Cell
Study Guide: The Cell

... 4. What are the 3 essential functions of DNA (In the text, they compared this to a book)? 5. DNA is a _________________________ made up of many small repeating units called ________________________. ...
DNA Repair - College of Arts and Sciences at Lamar University
DNA Repair - College of Arts and Sciences at Lamar University

... -Many environmental agents attack and modify DNA Thus maintenance of the genetic information requires constant repair of DNA damage ...
The purines In DNA, the pyrimidine bases are
The purines In DNA, the pyrimidine bases are

amp R - Fort Bend ISD
amp R - Fort Bend ISD

... Challenges: More proteins than genes Proteins differ with cell type and state Proteins are extremely variable in structure and function ...
Match each macromolecule (Carbohydrates, Proteins, Lipids
Match each macromolecule (Carbohydrates, Proteins, Lipids

... the cell membrane, other examples include vitamins, cholesterol, estrogen, and testosterone. _________________________________ ...
DNA/RNA Worksheet TACGGCACCGTTAGGATT
DNA/RNA Worksheet TACGGCACCGTTAGGATT

... Is DNA double-stranded or single-stranded? ________________________________________ ...
No Slide Title
No Slide Title

... A different colored dye can be used for each of the four reactions. ...
E. coli
E. coli

... 2 gene pairs: 50% recombinant gametes to detect recombinants in diploid organisms: use a test cross ...
Genetic Engineering pp 2014
Genetic Engineering pp 2014

... Sticky ends are used to make: Recombinant DNA – a piece of DNA made from the DNA of 2 different organisms Transgenic Organisms- organisms that contain recombinant DNA ...
a copy of the Candy DNA Replication
a copy of the Candy DNA Replication

... 2. Why is it important that DNA replicates? ______________________________________ 3. Why is it necessary for DNA to replicate accurately in a cell in order for an organism to survive? ____________________________________________________________________________ ______________________________________ ...
Practice Question for Replication, Genetics and Biotechnology
Practice Question for Replication, Genetics and Biotechnology

... 28. A trait that expresses itself as a heterozygote is ______________ (dominant or recessive). 29. Sex linked traits are found on the _____________________ chromosome. 30. People who have one copy of an allele for a recessive disorder, but do not exhibit symptoms are called _________ 31. Is blood ty ...
Archaebacteria Kingdom
Archaebacteria Kingdom

... ...
Prezentace aplikace PowerPoint
Prezentace aplikace PowerPoint

... A gene – what is it? • It is a molecular unit of heredity of a living organism. Genes are found in our chromosomes, which parents pass on to offspring in their sex cells in reproduction. Different versions of the same gene are called alleles and these can determine features like eye colour and the ...
No Slide Title
No Slide Title

... The ability to survive and reproduce in a particular ...
Study Questions for the Second Exam in Bio 0200
Study Questions for the Second Exam in Bio 0200

... Second Exam in Bio 0200 ...
Genetic Information
Genetic Information

... What causes mutations, can lead to cancer o High radiation, chemicals, high temperature Anything that can damage the cell DNA can fix itself, but if it is constantly exposed to a mutagen (ex. smoking) then it will not be able to fix the mutation Can result in cancer (cell keeps dividing) or ...
RNA
RNA

... RNA stands for ____________________________________ RNA takes the DNA’s instructions out of the __________________ and into the _______________________ of the cell where there is room for ____________________________________(protein synthesis) ...
genetics - Yazscience10
genetics - Yazscience10

... Genetic Code (2) • Human DNA contains enough information necessary to assemble about 100 000 different kinds of proteins • All known life forms use the same genetic code and same cellular mechanism to produce proteins • Humans share many genes with organisms that appear vastly different from us ...
Biotechnology
Biotechnology

... • What are these? • What are the uses? • What do they start with? ...
HtoN
HtoN

... can be used with other procedures to select cells and their DNA  May be of interest to a researcher ...
3rd Quarter Assessment Review - Belle Vernon Area School District
3rd Quarter Assessment Review - Belle Vernon Area School District

File
File

... a) some may carry oncogenes- genes to send cell cycle out of control b) some viruses may turn on protooncogenes at inappropriate times ...
problem set
problem set

... The two strands of the double-helical plasmid DNA separate (melt, denature) at 90˚C. During cooling down to 25˚C, the strands come back together. However, because the single-stranded DNA sequencing primer is in great excess, it hybridizes preferentially to its complementary region of the plasmid. Th ...
76d26f86fc8fd4690d9502156978f6866d36b66a
76d26f86fc8fd4690d9502156978f6866d36b66a

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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