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... Scientific Method & The Science of Biology  Steps of the scientific method; Hypothesis v. Theory; dependent v. independent variable; control group  Observations v. Inferences; Qualitative v. Quantitative Observations  Characteristics of life; Levels of organization ...
genetic recombination-unit-2-study material- 2012
genetic recombination-unit-2-study material- 2012

Chapter 12 SWBAT`s and Standards
Chapter 12 SWBAT`s and Standards

... Genes are a set of instructions encoded in the DNA sequence of each organism that specify the sequence of amino acids in proteins characteristic of that organism. As a basis for understanding this concept: a. ...
Unit 4 Review Sheet Genetics and Biotechnology Vocabulary
Unit 4 Review Sheet Genetics and Biotechnology Vocabulary

... - Do you know how to use the codon chart? - Why is the sequence of amino acids important to the shape and function of a protein? *You do NOT need to know the names of the enzymes involved in this process. Mutations - What is a mutation? - What kind of mutations can happen to DNA (i.e. a nucleotide i ...
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Q on Genetic Control of Protein Structure and function – Chapter 5

... Draw a diagram of a single DNA nucleotide. Which enzyme turns DNA nucleotides into a polynucleotide? Explain what is meant by “complementary base pairing”. What type of bond holds the two DNA strands together? What are the 2 essential functions of DNA? What are the 2 main types of RNA and what are t ...
Heredity and Genetics
Heredity and Genetics

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STUDY GUIDE FOR CHAPTER 12 – DNA Two Main Processes for
STUDY GUIDE FOR CHAPTER 12 – DNA Two Main Processes for

the nucleic acids - This is MySchool
the nucleic acids - This is MySchool

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Genes and Inheritance

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Nucleotide - Jackson County School District

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Psychgene - Schule.at

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Biotechnology Glow Genes

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GE & Profiling iQuiz

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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