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DNA Biology - De Anza College
DNA Biology - De Anza College

Genetic Engineering - Somers Public Schools
Genetic Engineering - Somers Public Schools

... What is genetic engineering Cont… • It often involves the isolation, manipulation and reintroduction of DNA into cells or model organisms, usually to express a protein. • The aim is to introduce new characteristics such as making a crop resistant to a herbicide, introducing a novel trait, or produc ...
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Genetic Engineering

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Unit VII Objectives Biotechnology
Unit VII Objectives Biotechnology

DNA REPLICATION HANDOUT
DNA REPLICATION HANDOUT

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Biotechnology - Hicksville Public Schools / Homepage
Biotechnology - Hicksville Public Schools / Homepage

... A gene is a sequence of ______ protein that codes for one __________. DNA codes for proteins. **Remember, not all of the ______ genes the parts that The parts that do are called ________, don’t are called _________________ non-coding regions. ...
Biology, Chapter 11 DNA and Genes Study Guide 1. What two
Biology, Chapter 11 DNA and Genes Study Guide 1. What two

... 18. How can DNA with only 4 bases code for proteins made up of 20 different amino acids? 20. Translate a given mRNA sequence into an amino acid sequence. ...
Molecular Genetics (Unit 6 and Unit 6.2) Study Guide Each of the
Molecular Genetics (Unit 6 and Unit 6.2) Study Guide Each of the

DNA Notes - Firelands Local Schools
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Lab Quiz 4 Key

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BIO 112 Review - Crossword Labs

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Unit 1 - Glen Rose FFA

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NAME CH. 8 HONORS STUDY GUIDE SCIENTISTS: Hershey

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GBE 214 TECNIQUES IN MOLECULAR BIOLOGY
GBE 214 TECNIQUES IN MOLECULAR BIOLOGY

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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