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the VECTOR (gene carrier)
the VECTOR (gene carrier)

... 5.) DNA polymerase is used to synthesize a second DNA strand. The DNA that results from such a procedure, called COMPLEMENTARY DNA (cDNA), represents only the subset of genes that had been transcribed into mRNA in the starting cells. ...
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... and traits that are best fit for the environment are passed on. ...
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... • The cell - a basic unit of life containing living material bound by a membrane. The cell also contains genetic material. • 1. Within each cell there is a nucleus that contains genetic material. • 2. The long molecule that carries the genetic material is DNA or deoxyribonucleic acid. • 3. The DNA ...
Release Test items 11th Grade Obj 2
Release Test items 11th Grade Obj 2

... B.6C – Identify and illustrate how changes in DNA cause mutations [and evaluate the significance of these changes]. Mutations in DNA molecules can occur when — F replication of DNA is exact G a DNA enzyme attaches to an RNA codon H RNA codons are replaced by DNA nucleotides J a change occurs in DNA ...
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Working with Data The Hershey–Chase Experiment

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recombinant DNA - Cloudfront.net
recombinant DNA - Cloudfront.net

... • genetic engineering is possible because of restriction enzymes (restriction endonucleases): • Very specific – recognize and then cut DNA molecules at specific base sequences called a restriction site (recognition sequence) – These are often a symmetrical series of four to eight bases on both stra ...
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direct genetic testing

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Biology - TeacherWeb

... The process of converting the information in a sequence of nitrogenous bases in mRNA into a sequence of amino acids in protein 33. What is mutations? Any change or error in the DNA sequence 34. Explain how mutations in body cells cause damage. If the cell’s DNA is changed, the mutation would be pass ...
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... 7. Why is the single-strand binding protein needed in DNA replication? 8. With few exceptions, all nuclei of eukaryotes contain A. Genes to specify the portion of the organism in which they are found B. All of the information needed for growing the whole organism C. All of the chromosomes except sex ...
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... the number and relative positions of target sites along the DNA can be determined for each restriction enzyme. The resulting map can be used to determine the smallest restriction fragment containing an intact gene. (Finding the gene among the fragments requires additional techniques that will not be ...
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... disorder in which some of the body's own cells cannot stop dividing; they have lost the ability to control the cell cycle ...
talk_DNAEditing
talk_DNAEditing

... 4. Insertion into new genomic locations, increasing the number of genomic copies of the sequence. • Mobile elements are like double edge sword. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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